Polymeric sorbent for removal of impurities from whole blood and blood products
A technology for blood products and polymers, applied in the fields of blood diseases, selective adsorption, alkali metal compounds, etc., can solve problems such as increased risk of death
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Embodiment 1
[0043] Embodiment 1 Adsorbent 1~11 is synthesized
[0044] The reactor was set up such that the kettle (0.5 L) was equipped with an over head stirrer, multi-level stirrer blades, water cooled condenser, thermocouple and bubbler. Install the gasket between the top cover and bottom kettle. Plug all unused ports with appropriate plugs. The temperature was controlled with a heating mantle regulated by a temperature controller equipped with the above-mentioned thermocouples.
[0045] Polymerization, polyvinyl alcohol was dispersed in a water load at room temperature (RT), followed by heating to 70°C. The remaining salts (see Table 1, MSP, DSP, TSP & Sodium Nitrite) were then dissolved in the water load. The PVA and salt solution was heated to 80°C and stirred. The premixed organic phase listed in Table 2 including the initiator was poured onto the aqueous phase in the reactor and the stirring speed set in rpm for proper droplet size formation. Once the temperature reached a ...
Embodiment 2
[0050] Embodiment 2 hole structure characteristic
[0051] The pore structure of the adsorbent polymers was analyzed with a granulometry AutoPore IV 9500 VI.09 Mercury Penetrometer (Hg Intrusion Instruments). The result is as figure 1 , where pore volume is plotted as a function of pore diameter.
Embodiment 3
[0052] The comparison of embodiment 3 pore structure and biomolecule adsorption
[0053] The pore structure of the adsorbent polymer was compared with cytochrome c, human serum albumin and immunoglobulin G (IgG). Cytochrome C (~12 kilodaltons) is used as a surrogate for intermediate molecular weight proteins such as cytokines, human serum albumin (67 kilodaltons) is used as a surrogate for hemoglobin (64 kilodaltons), and IgG stands for antibody. The comparison is shown in Table 3.
[0054] table 3
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[0056]
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