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A kind of preparation method of modified polyester material

A polyester, modified technology, applied in the field of polyester material preparation, to prevent thrombus blockage, reduce injury, facilitate tissue healing and anti-coagulation effects

Active Publication Date: 2017-08-11
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current clinical problems of polyester large-diameter artificial blood vessels and the fundamental problem that it cannot be applied to the preparation of small-diameter artificial blood vessels, the present invention aims to provide a method for preparing biologically expressed polypeptides and modified polyester materials. Modified polyester is a blood-compatible material with excellent surface hydrophilicity and negative charge. It can be used to prepare implants that are in direct contact with blood (such as artificial blood vessels, heart patches, artificial heart valves, etc.). Facilitates tissue healing and anticoagulation

Method used

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  • A kind of preparation method of modified polyester material
  • A kind of preparation method of modified polyester material
  • A kind of preparation method of modified polyester material

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 1. Transfect Escherichia coli (BL21) cells with the prokaryotic system expression vector carrying the first polypeptide F1 gene, coat it on Luria-Bertani solid medium containing ampicillin, put it upside down and put it in a biochemical incubator at 37°C for 14 ~16 hours; pick a single colony and put it into 4mL Luria-Bertani liquid medium containing fresh ampicillin, insert it into an air shaker with a shaking speed of 200r / min at 37°C for 8-10 hours; Amplify and culture in 1L fresh Luria-Bertani liquid medium containing ampicillin at a ratio of 1:100; when the density of the bacterial solution reaches OD 600 When = 0.3-1.8, 0-1.2 mM isopropyl-β-D-thiogalactoside was added to induce culture for 1-8 hours, and the bacterial cells were collected by centrifugation at 4°C.

[0020] 2. Resuspend and mix the collected bacterial cells with the binding buffer of glutathione transferase (GST) affinity chromatography, and put them on ice to ultrasonically disrupt the cells and r...

Embodiment 2

[0026] 1. Transfect Escherichia coli (BL21) cells with the prokaryotic system expression vector carrying the first polypeptide F1 gene, coat it on Luria-Bertani solid medium containing ampicillin, put it upside down and put it in a biochemical incubator at 37°C for 14 ~16 hours; pick a single colony and put it into 4mL Luria-Bertani liquid medium containing fresh ampicillin, insert it into an air shaker with a shaking speed of 200r / min at 37°C for 8-10 hours; Amplify and culture in 1L fresh Luria-Bertani liquid medium containing ampicillin at a ratio of 1:100; when the density of the bacterial solution reaches OD 600 When =0.3-1.8, add 0-1.2mM isopropyl-β-D-thiogalactoside to induce culture for 1-8 hours, and collect bacterial cells by centrifugation at 4°C.

[0027]2. Resuspend and mix the collected bacterial cells with the binding buffer of glutathione transferase (GST) affinity chromatography, put them on ice and ultrasonically break the cells to release the protein; after ...

Embodiment 3

[0033] 1. Transfect Escherichia coli (BL21) cells with the prokaryotic system expression vector carrying the second polypeptide F4 gene, coat it on the Luria-Bertani solid medium containing ampicillin, and place it upside down in a biochemical incubator at 37°C for 14 ~16 hours; pick a single colony and put it into 4mL Luria-Bertani liquid medium containing fresh ampicillin, insert it into an air shaker with a shaking speed of 200r / min at 37°C for 8-10 hours; Amplify and culture in 1L fresh Luria-Bertani liquid medium containing ampicillin at a ratio of 1:100; when the density of the bacterial solution reaches OD 600 When = 0.3-1.8, 0-1.2 mM isopropyl-β-D-thiogalactoside was added to induce culture for 1-8 hours, and the bacterial cells were collected by centrifugation at 4°C.

[0034] 2. Resuspend and mix the collected bacterial cells with the binding buffer of glutathione transferase (GST) affinity chromatography, put them on ice and ultrasonically break the cells to release...

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Abstract

The invention discloses a preparation method of a modified polyester material. The specific steps are as follows: 1) Transfect Escherichia coli cells with a prokaryotic system expression vector carrying a gene having a hydrophilic and negatively charged polypeptide, and induce Cultivate for several hours; 2) collect and break the cells, obtain hydrophilic and strongly negatively charged first polypeptide F1, second polypeptide F4 or third polypeptide F8 after purification, and prepare functional polypeptide aqueous solution; 3 ) After the polyester is treated with sodium hydroxide, it is washed with deionized water, then immersed in a functional polypeptide aqueous solution, and a cross-linking agent is added to react overnight at 4°C to obtain a modified polyester material. The modified polyester prepared by the method of the present invention belongs to blood-compatible materials, has excellent surface hydrophilicity and negative charge, can be prepared and applied to implants in direct contact with blood, and can reduce damage to cells. It is conducive to endothelialization, prevents protein deposition and blood cell aggregation from causing thrombus blockage, and is beneficial to tissue healing and anticoagulation.

Description

technical field [0001] The invention relates to a preparation method for a polyester material used in blood contact, in particular to a preparation method for introducing a hydrophilic and negatively charged polypeptide into the surface of a polyester material. Background technique [0002] There are millions of deaths due to cardiovascular and cerebrovascular diseases in my country every year, and the proportion is increasing year by year by about 30%. Therefore, vascular transplantation has become a hot spot of concern. Artificial blood vessels are currently the most scarce medical devices in clinical practice. Among them, the transplantation of small-caliber artificial blood vessels is still a clinical blank. Even for medium and large-caliber artificial blood vessels, there are very few products in my country. The annual use ratio of domestic products is very small, only 20 %about. It is conservatively estimated that more than 2 million (about 600,000 small-caliber) patie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06M16/00C12N15/70C07K14/435A61L27/18A61L27/22A61L27/50D06M101/32
Inventor 王建南杨高强裔洪根
Owner SUZHOU UNIV