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Microfluidic chip used for single-cell-impedance flow detection and processing method thereof

A microfluidic chip, flow detection technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, enzymology/microbiology devices, etc., can solve the problem of increasing design and operation complexity, disadvantageous Noise suppression, reducing cell detection sensitivity and other issues, to avoid aligned substrate bonding, cost reduction, and avoid dry etching process

Active Publication Date: 2019-06-07
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing technology reported in the literature uses a single pair of detection electrodes instead of a differential electrode design, which is not conducive to noise suppression; and the cross-sectional area and spacing of the detection electrodes are much larger than the cell diameter, which reduces the sensitivity of cell detection
In addition, due to the large distance between electrodes, the design introduces a sheath flow focusing structure to stabilize the position of cells passing through the electrodes to increase the stability of the signal, but the introduction of sheath flow increases the complexity of design and operation

Method used

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  • Microfluidic chip used for single-cell-impedance flow detection and processing method thereof

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Embodiment 1

[0062] see details Figure 1-7 ;

[0063] Such as figure 1 Shown is a schematic structural diagram of a microfluidic chip for single-cell impedance flow detection according to a specific embodiment of the present invention.

[0064] The chip structure includes PDMS top layer 1, AgPDMS electrode-channel layer 2 and glass lower substrate layer 3 from top to bottom. The height of the PDMS top layer 1 is 2-6 mm, and the diameter of the hole 4 used for cannula injection and cannula collection of the sample to be tested in the top layer 1 is 0.75-2 mm.

[0065] Such as figure 2 Shown is a schematic diagram of the electrode-channel layer structure of the microfluidic chip for single-cell impedance flow detection according to a specific embodiment of the present invention. The two halves of the electrode-flow channel layer 2 are respectively provided with a grooved through hole, respectively recorded as the sample inlet 5 and the sample outlet 6, the diameter of the sample inlet ...

specific Embodiment approach

[0094] Using polystyrene microspheres as a cell model, an impedance analysis test is performed on the chip, and impedance test signals are collected simultaneously at multiple frequencies. Specific implementation methods include:

[0095] Step 1. Connect the chip and signal processing equipment:

[0096] The electrical signal connection transmission between the chip microelectrode and the subsequent signal processing equipment adopts the following method: smear silver glue on the end side of each electrode wide cross-section section 14 of the three-dimensional electrode 9 located at the edge of the chip, and connect the silver glue with a One end of the thin soft copper wire is connected, and the other end of the wire is welded on the PCB board and transferred to the SMA interface by the wiring on the PCB board. A pair of three-dimensional electrodes on the same side of the linear flow channel of the chip receives the excitation voltage 16 from the excitation output terminal ...

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Abstract

The invention discloses a microfluidic chip used for single-cell-impedance flow detection and a processing method thereof, and relates to the technical field of cell detection and analysis chips. Themicrofluidic chip used for the single-cell-impedance flow detection comprises a top layer, an electrode-flow channel layer, and a lower basal plate layer. The lower basal plate layer comprises a flowchannel, and two pairs of differential electrode pairs; the flow channel comprises an input section, an output section and a middle detection section; and the differential electrode pairs are insertedinside the middle detection section, share the same height with the side wall of the flow channel, and are rightly placed face to face. Three-dimensional self-aligning electrodes require no aligned substrate bonding of conventional metal film electrodes; moreover, configuration of the differential electrode pairs, as well as intra-electrode detection areas of the three-dimensional electrodes rightly placed face to face which are close to volumes of cells to be measured, facilitates improvement of cell D detection sensitivity. In terms of processes, the whole electrode-flow channel layer is formed by performing casting with the same material, so that, extra sacrificial layer lithography caused by electrodes and flow channels constructed by different materials in the same components is avoided; and thus, the processes are greatly simplified with cost reduced.

Description

technical field [0001] The invention relates to the technical field of cell detection and analysis chips, in particular to the structural design and processing flow of a microfluidic chip for unlabeled flow single cell detection. Background technique [0002] Cell heterogeneity is a common feature of biological systems, and the detection of heterogeneous minority subpopulations or even single cells in the cell population is of great significance in the fields of life science research and medical diagnosis. For example, in the research of diseases such as cancer and immunity, it is often the few mutant cells in the population that are most relevant to the research object. Traditional cell detection methods are mostly aimed at the average properties of cell populations, resulting in the neglect of cell heterogeneity. Therefore, single-cell detection technology is particularly important for detecting the dynamic changes of cell populations and revealing individual differences a...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
Inventor 邢晓星梁自恒
Owner BEIJING UNIV OF CHEM TECH