Preparation method and application of coumarin glycoside compounds

A technology of coumarin glycosides and compounds, applied in the field of pesticides, to achieve significant economic and social benefits and good application value

Active Publication Date: 2019-06-14
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The coumarin glycoside compounds involved in the present invention and t...

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  • Preparation method and application of coumarin glycoside compounds
  • Preparation method and application of coumarin glycoside compounds
  • Preparation method and application of coumarin glycoside compounds

Examples

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Embodiment 1

[0012] In the specific implementation of the present invention, a kind of coumarin glycoside compound, the compound is made of 20kg of small bark bone as a raw material, and is extracted 3 times by heating at 90°C with ethanol with 2-5 times the weight of the raw material and a volume concentration of 95%. The extraction time is 1 hour, and ethanol is recovered under reduced pressure to obtain extract-like ethanol extract, which is suspended in 4.8L of distilled water, and extracted 3 times with petroleum ether, ethyl acetate, and n-butanol successively, 4.8L each time, The time is 1.5 hours; the n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, each One gradient uses 5.0L eluent, the flow rate is 10mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respe...

Embodiment 2

[0014] In the specific implementation of the present invention, a kind of coumarin glycoside compound, with 15kg of small barb bone as raw material, is 3 times of raw material weight, and volume concentration is 75% ethanol heating 95 ℃ of reflux extraction 3 times, and each extraction time is For 1 hour, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 3.6L of distilled water, and extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence, 3.6L each time, for 1.5 hours ; The n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, and each gradient uses 3.8 L eluent, the flow rate is 5mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respectively, concentrated and dried under ...

Embodiment 3

[0016] In the specific implementation of the present invention, a kind of coumarin glucoside compound, with 18kg of small barb bone as raw material, with 4 times of raw material weight, volume concentration is 85% ethanol heating 92 ℃ of reflux extractions 3 times, each extraction time is After 1 hour, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 4.5L of distilled water, and extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence, 4.5L each time, for 1.5 hours ; The n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, and each gradient uses 4.5 L eluent, the flow rate is 8mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respectively, concentrated and dried under red...

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Abstract

The invention relates to a preparation method and application of coumarin glycoside compounds. The preparation method can effectively achieve the technical effects of preparing the novel coumarin glycoside compounds and achieving the preparation of antibacterial drugs. The compounds are genglycoside C (I), genglycoside E (II) and genglycoside F (III) which are separated from a medicinal material of common gendarussa herb. The prepared novel coumarin glycoside compounds genglycoside C (I), genglycoside E (II) and genglycoside F (III) which have antimicrobial activity serve as the only active ingredients to be effectively used for preparing pesticides resisting agrobacterium tumefaciens, open up a new path for antibacterial pesticides and the medicinal value and commercial value of the common gendarussa herb, and have higher practical application value and significant economic and social benefits.

Description

technical field [0001] The invention relates to pesticides, in particular to a preparation method and application of coumarin glycoside compounds. Background technique [0002] The widespread use of chemically synthesized pesticides not only pollutes the environment, but also accelerates the emergence of drug-resistant bacteria, and even multidrug-resistant bacteria. Botanical pesticides have different structural characteristics. Due to the complexity and diversity of the structure, they have incomparable advantages in the field of pesticides, such as fast biodegradation, low toxicity, not easy to produce resistance, and no pollution to the environment. It has attracted more and more attention from researchers. Therefore, searching for new pesticides with antibacterial activity with high efficiency and low toxicity from Chinese herbal medicines, and developing antibacterial pesticides with strong selectivity and low toxic and side effects are the most urgent problems for ph...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08A01P1/00
Inventor 孙彦君高美玲陈豪杰冯卫生李孟杨静万焱王俊敏张艳丽韩瑞杰赵晨
Owner HENAN UNIV OF CHINESE MEDICINE
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