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Peach polypeptide hormone synthesis gene pprgf1 and its application

A technology of polypeptide hormones and synthetic genes, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as fruit softening and unclear interaction mechanism, and achieve the effect of promoting ripening

Active Publication Date: 2022-07-12
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further studies found that in MF peach, auxin-induced ACS1 Gene expression, which in turn induces ethylene synthesis, resulting in fruit softening
Auxin and ethylene jointly regulate the ripening and softening of peach fruit. However, the interaction mechanism between auxin and ethylene is still unclear. The study found a peach gene encoding a polypeptide hormone similar to GOLVEN CTG134 It plays a regulatory role in fruit ripening. This gene is acquired in the mesocarp of peach fruit during ripening, expressed during respiratory transition, and up-regulated during peach fruit ripening. At the same time, this gene responds to NAA and 1-MCP treatment, suggesting that write GLV May play an intermediate regulatory role between auxin and ethylene

Method used

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  • Peach polypeptide hormone synthesis gene pprgf1 and its application
  • Peach polypeptide hormone synthesis gene pprgf1 and its application
  • Peach polypeptide hormone synthesis gene pprgf1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Peach polypeptide hormone synthesis gene PpRGF1 isolation and identification of

[0034] experiment method

[0035] 1. Gene PpRGF1 separation

[0036] The peach pulp RNA was extracted with a plant polysaccharide and polyphenol kit (DP441, Tiangen Biochemical Technology (Beijing) Co., Ltd.), and single-stranded cDNA was obtained by a reverse transcription kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.). cDNA was used as the template, and the following sequences were used as primers, and the gene was obtained by PCR (the kit FastStart SYBR Green Master was purchased from Roche Biotechnology Co., Ltd.) PpRGF1 The full-length sequence of the PCR amplification electropherogram is shown in figure 1 shown. Gene PpRGF1 The full-length sequence is shown in SEQ ID NO: 1 in the sequence table, with a total of 489 bp, and the amino acid sequence of the co-encoded protein is shown in SEQ ID NO: 2 in the sequence table, with a total of 162. Peach PpRGF1 wi...

Embodiment 2

[0067] Example 2 Gene PpRGF1 The role of transgenic tomatoes

[0068] 2.1 Genes PpRGF1 Effects on tomato fruit development

[0069] Image 6 Observation diagrams of wild-type tomato and transgenic tomato fruits at different developmental stages. from Image 6 It can be seen that the transgenic tomato began to change color at 41d after flowering, and the wild-type tomato began to change color at 45d after flowering, that is, the transgenic tomato matured about 4d earlier than the wild-type tomato. same.

[0070] 2.3 Genes PpRGF1 Effects on tomato fruit firmness and ethylene release

[0071] The ethylene levels of transgenic and wild-type tomato fruits at different days after flowering (37dpa, 41dpa, 45dpa, 50dpa, 53dpa, 59dpa) were measured. Figure 7 Comparison of ethylene release changes of wild-type tomato and transgenic tomato fruits at different ripening stages. from Figure 7 It can be seen that the ethylene release of wild-type and transgenic tomato showed a tr...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and relates to a peach polypeptide hormone synthesis gene PpRGF1 and its applications. The present invention adopts the cloning of peach polypeptide hormone GLV gene family members get PpRGF1 , and analyzed the expression pattern of the gene in the process of solute peach fruit ripening, and finally transferred it into Micro-Tom tomato by Agrobacterium-mediated method to verify the function of the target gene, which is beneficial to clarify the molecular mechanism in the peach ripening process. The role played by polypeptide hormones in peach has a positive guiding role in further realizing the purposeful and targeted quality and character improvement of peach and cultivating new peach varieties.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and relates to a peach polypeptide hormone synthesis gene PpRGF1 and its applications. Background technique [0002] Melting flesh (MF) fruit is a climacteric fruit. Under the action of ethylene, the fruit softens rapidly and reaches commercial maturity. The hard peach (stony hard, SH) fruit does not release ethylene during ripening, and does not soften whether it is left or harvested under the condition of complete color change and accumulation of high concentrations of sugar. The study found that the ethylene synthesis of SH peach was blocked due to the key gene of ethylene synthesis ACS1 The expression of MF peach fruit was always at a low level, while the ACS1 Gene expression increased rapidly with fruit ripening. Further research found that in MF peach, auxin induced ACS1 Gene expression, which in turn induces ethylene synthesis, results in fruit softening. Auxin a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00A01H6/82
Inventor 曾文芳王志强王雁牛良潘磊鲁振华崔国朝王小贝邓丽
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI