Application of sodium ion channel blocker in preparation of drugs to treat melanoma
A sodium ion channel and melanoma technology, applied in drug combinations, antineoplastic drugs, pharmaceutical formulations, etc., can solve problems such as the application of sodium ion channel blockers that have not yet been discovered, and achieve shortened development time, target specificity, reduced side effects and The effect of drug resistance
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Embodiment 1
[0035] Tetrodotoxin, dehydrated tetrodotoxin, and combinations of dehydrated tetrodotoxin and tetrodotoxin inhibit melanotic solid tumors in mice
[0036] (1) Experimental materials
[0037] (1) Melanoma cell line: melanoma cell B16F10 is cryopreserved in our laboratory;
[0038] (2) Test drugs: dehydrated tetrodotoxin, tetrodotoxin, crude tetrodotoxin (composition representing dehydrated tetrodotoxin and tetrodotoxin), cisplatin freeze-dried powder and normal saline;
[0039] (3) Experimental animals: clean grade ICR mice, half male and half male.
[0040] (2) Experimental method
[0041] B16F10 melanoma cells were resuscitated and cultured in vitro. After passage and expansion, the cells in the logarithmic growth phase were collected, suspended in DMEM liquid medium containing 10% fetal calf serum, and inoculated with the cell suspension in the right axilla of each mouse. Liquid 0.2ml (1×10 6 tumor cells). On the day after melanoma cell inoculation, the inoculated mice ...
Embodiment 2
[0052] Dehydrated tetrodotoxin, tetrodotoxin and crude tetrodotoxin inhibit human melanoma cell lines
[0053] (1) Experimental materials
[0054] (1) Melanoma cell line: melanoma cell G-361 was purchased from ATCC in the United States;
[0055] (2) Main test drugs: dehydrated tetrodotoxin, tetrodotoxin, acetic acid and cisplatin freeze-dried powder.
[0056] (2) Experimental method
[0057] The cytotoxic effect of test sample A (tetrodotoxin dehydrate) and test sample B (tetrodotoxin) on human melanoma cell line G-361 was investigated by MTS method.
[0058] Human melanoma cell G-361 was made into a single-cell suspension with McCoy's 5A (containing 2 mM glutamine) liquid medium containing 10% fetal calf serum by volume fraction, and the human melanoma cell G-361 was diluted 2×10 3 Each well was inoculated in a 96-well plate, 90 μl per well. After conventional culture for 24 hours, 10 μl of dehydrated tetrodotoxin solution of different concentrations were added to each we...
Embodiment 3
[0069] Combination of dehydrated tetrodotoxin and tetrodotoxin with trametinib or cabitinib inhibits the proliferation of human melanoma cells
[0070] (1) Experimental materials
[0071] (1) Melanoma cell line: melanoma cell A375 (CRL1619IG-3) was purchased from ATCC, USA;
[0072] (2) The main test drugs: dehydrated tetrodotoxin (A), tetrodotoxin (B), trametinib (D), cabetinib (E), acetic acid and normal saline.
[0073] (2) Experimental method
[0074] Using CCK8 method to investigate dehydrated tetrodotoxin, tetrodotoxin, trametinib, cabetinib, combination of dehydrated tetrodotoxin and trametinib, combination of tetrodotoxin and trametinib, combination of dehydrated tetrodotoxin and cabetinib, And the cytotoxic effect of tetrodotoxin combined with carbitinib on human melanoma cell line A375.
[0075] The melanoma cell line A375 was made into a single-cell suspension with DMEM liquid medium containing 10% fetal calf serum by volume fraction, and the melanoma cell line A...
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