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Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application for kiwifruit anthracnose control

A technology for photosynthetic bacteria and biocontrol agents, which is applied in the field of photosynthetic bacteria strains and achieves the effects of simple production process, less resistance to drug resistance, and simple and convenient application.

Active Publication Date: 2020-12-18
湖南喜多收生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a photosynthetic bacterial strain, a biocontrol agent, a biocontrol fermentation liquid, a preparation method and application for the prevention and treatment of kiwifruit anthracnose, so as to solve the technical problems of the prevention and treatment of kiwifruit anthracnose

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  • Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application for kiwifruit anthracnose control
  • Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application for kiwifruit anthracnose control

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preparation example Construction

[0032] A preparation method for a photosynthetic bacteria biocontrol agent for the prevention and treatment of kiwifruit anthracnose, comprising the following steps:

[0033] Activation: culture the preserved species of photosynthetic bacteria strain Rhodobacter vaninii B-30 according to the double-layer plate culture method, and cultivate until red single colonies appear;

[0034] Seed culture: insert the red single colony into the photosynthetic bacteria liquid medium for seed culture until the logarithmic growth phase to obtain the bacterial liquid; refer to figure 2 .

[0035] Production culture: The bacteria liquid is inoculated into the photosynthetic bacteria production medium according to the inoculum amount of 5% of the total volume of the photosynthetic bacteria strains, and the production culture is carried out until the logarithmic growth phase to obtain the photosynthetic bacteria biocontrol agent.

[0036] In the seed culture stage, the red single colony can be...

Embodiment 1

[0050] A photosynthetic bacteria strain named as photosynthetic bacteria Rhodomicrobium vanneii B-30.

[0051] The photosynthetic bacteria strain Rhodobacter vanneii B-30 is Gram-negative bacteria, which is identified as photosynthetic bacteria by physiological and biochemical identification and molecular biology. The main biological characteristics are: cultured on double-layer solid plate medium for 4d to 6d, forming red circular colonies with neat and smooth edges and colony diameter of 0.2mm to 0.60mm; cultured in liquid medium for 5d to become dark red; It grows well under oxygen and micro-aerobic conditions; the physiological and chemical characteristics are V-P reaction and methyl red reaction negative, H 2 S reaction, gelatin liquefaction, urease test and indole test were all positive, and the optimum growth temperature was 30℃~35℃, pH=7.

Embodiment 2

[0053] A kind of photosynthetic bacterium biocontrol bacterium agent, adopts the photosynthetic bacterium bacterial strain Rhodobacter vaninii B-30 of embodiment 1 to be raw material, obtains through activation, seed culture, production culture and prepare, and its specific preparation method comprises the following steps:

[0054] (1) Activation: the preserved species of the photosynthetic bacterium Rhodomicrobium vaninii B-30 bacterial strain is cultivated by the double-layer plate culture method, and it is cultivated until red single colony occurs; the culture medium adopted is a photosynthetic bacteria solid medium, and the formula is :(NH 4 ) 2 SO 4 0.5g / L, CH 2 COONa 0.5g / L, YE 1.5g / L, K 2 HPO 4 About 1g / L (according to the actual situation when the pH value is adjusted to 7, the dosage shall prevail), FeSO 4 0.05g / L, H 3 BO 4 0.05g / L, pH=7.0.

[0055] (2) Seed culture: Put the red single colony cultivated in step (1) into a 120mL serum bottle, and use photosy...

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Abstract

The invention discloses a photosynthetic bacteria strain, a biocontrol bacterial agent, a biocontrol fermentation liquid for treating and preventing anthracnose of kiwi fruits, and a preparation method and application. The photosynthetic bacteria strain is Rhodomicrobium vannielii B-30. The production process of photosynthetic bacteria is simple, the cultivation time is short, and the cost is low;the strain can be prepared into the biocontrol bacterial agent and the biocontrol fermentation liquid for treating and preventing anthracnose of kiwi fruits according to a simple, rapid and low-costpreparation method; the biocontrol bacterial agent and the biocontrol fermentation liquid, which are prepared from the photosynthetic bacteria strain, have the advantages of environmental friendliness, no toxicity on human and animals, no phytotoxicity on crops, simplicity and convenience in application, and the like, are not likely to cause drug resistance of insect pests, and can prevent and treat the anthracnose of the kiwi fruits and promote the growth of the kiwi fruits.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a photosynthetic bacterial strain, a biocontrol agent, a biocontrol fermentation liquid, a preparation method and an application for the prevention and treatment of kiwifruit anthracnose. Background technique [0002] Kiwifruit anthracnose is one of the main diseases that harm kiwifruit production. The disease mainly damages kiwifruit leaves and fruits, and also infects branches. Anthracnose can occur throughout the growth period of kiwifruit, and the damage is more severe from the early stage of fruiting to the leaf defoliation stage. The causative agent is Colletotrichum sp. Mainly damage stems, leaves and fruits, affecting plant growth and fruit quality. In some orchards in kiwi fruit producing areas, the disease incidence rate reaches more than 95%, the plant disease rate in a few orchards is more than 80%, and the leaf disease rate is 15% to 85%. In the orchard dise...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
Inventor 戴建平苏品周波张卓唐雯杜晓华程菊娥翟忠英张德咏刘勇
Owner 湖南喜多收生物科技有限公司