Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of adenosine in preparation of drugs or reagents for promoting embryo formation or improving embryo quality

An embryo quality and adenosine technology, applied in the field of medicine, can solve the problem of little research on granulosa cell metabolism, and achieve the effect of improving oocyte quality and embryo development, reducing meiotic defects, and decreasing ROS levels.

Active Publication Date: 2019-06-21
THE SECOND AFFILIATED HOSPITAL OF NANJING MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the metabolism of granulosa cells. Therefore, finding and establishing a credible metabolomics embryo evaluation and prediction model to improve embryo formation and embryo quality is the goal that assisted reproduction researchers have been pursuing, and it will be greatly promoted. Application of Metabolomics Analysis in IVF

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of adenosine in preparation of drugs or reagents for promoting embryo formation or improving embryo quality
  • Application of adenosine in preparation of drugs or reagents for promoting embryo formation or improving embryo quality
  • Application of adenosine in preparation of drugs or reagents for promoting embryo formation or improving embryo quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Effect of Adenosine on Mouse Embryo Development

[0038] Take 8-week-old ICR mice, inject PMSG, inject hCG 48 hours later, and collect materials 16 hours later; obtain hyaluronidase-digested granulosa cells from the oviduct ampulla under a microscope, clean the naked eggs, and distribute them evenly at 37°C for 5 %CO 2 Well-balanced M2 (Aged: control group); M2 added with DMSO (Aged+D (Aged+DMSO): solvent control group) and M2 added with 10 μM adenosine dissolved in DMSO (Aged+A (Aged +Adenosine): In the experimental group) in the culture medium, in vitro fertilization was carried out after 12 hours. Simultaneously, fresh oocytes (Fresh: fresh control group) were taken out immediately for in vitro fertilization. The sperm of the 4 groups for in vitro fertilization all came from the same male mouse. Observing the embryonic development process, and performing fluorescent staining statistics on the blastocysts, it was found that the formation of early embryos ...

Embodiment 2

[0039] Example 2 Changes of intracellular adenosine content and transport carriers after exogenous addition of adenosine

[0040] Take 8-week-old ICR mice, inject PMSG, inject hCG 48 hours later, and collect materials 16 hours later; obtain hyaluronidase-digested granulosa cells from the oviduct ampulla under a microscope, clean the naked eggs, and distribute them evenly at 37°C for 5 %CO 2 Well-balanced M2 supplemented with DMSO (Aged+D (Aged+DMSO): solvent control group), M2 supplemented with 10 μM adenosine dissolved in DMSO (Aged+A (Aged+Adenosine): experimental group 1) and Added M2 (Aged+A+C (Aged+Adenosine+Caffeine): experimental group 2) culture solution with 10 μM adenosine and caffeine dissolved in DMSO, washed the naked eggs with PBS after 12 hours, and collected them. At the same time, oocytes in the Fresh group (Fresh: fresh control group) were immediately taken out, digested with hyaluronidase, washed with PBS, and then collected. The 4 groups of samples were a...

Embodiment 3

[0041] Example 3 Detection of adenosine content in human granulosa cells and follicular fluid and the effect of adenosine on oocyte quality

[0042] The granulosa cells and follicular fluid of the IVF population with normal epidemiological information were collected, grouped according to the embryo quality on the third day, and divided into a high-quality group and a low-quality group with a high-quality embryo rate of 40%. Adenosine content, showing that the low-quality group adenosine content increased in granulosa cells and decreased in follicular fluid ( image 3 ).

[0043] Since the adenosine content in the follicular fluid of the low-quality group decreased, and considering that the follicular fluid is the external environment of the oocyte, we added adenosine (the appropriate concentration was 10 μM) to the in vitro culture medium of the oocyte in the animal experiment. Take 8-week-old ICR mice, inject PMSG, inject hCG 48 hours later, and collect materials 16 hours la...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicines, and particularly relates to application of adenosine in preparation of drugs or reagents for promoting embryo formation or improving embryo quality. When the adenosine is applied to preparation of the drugs or reagents for promoting embryo formation or improving embryo quality and preparation of an embryo culture solution, ROS level of oocytes is reduced, meiosis defects are reduced, the embryo quality is improved, and blastocyst quality is improved. It shows that if the adenosine is added in an embryo formation process, biological functions ofpromoting embryo formation or improving the embryo quality are fulfilled.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the application of adenosine, in particular to the application of adenosine in the preparation of medicines or reagents for promoting embryo formation or improving embryo quality. Background technique [0002] After more than 30 years of development and improvement, conventional in vitro fertilization-embryo transfer technology has technically solved long-term medical problems such as infertility. However, in the conventional treatment of in vitro fertilization-embryo transfer, in order to ensure a certain clinical pregnancy rate, the Transfer of 2-3 cleavage-stage embryos is a common practice. Therefore, in the past 30 years, the overall twin pregnancy rate of the population has increased by 50%-70%, and the triple pregnancy rate has increased by 400%. However, multiple pregnancy has seriously threatened the safety of mothers and babies. In recent years, maternal complications during pregna...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 钱云王晖杨继虹冯婷
Owner THE SECOND AFFILIATED HOSPITAL OF NANJING MEDICAL UNIV