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Simple and effective method for non-destructive observation of root nodules and root morphology

A root system, a simple technology, applied in the field of non-damage observation of root nodules and root system morphology, can solve the problems of not being able to grasp the root nodule's tropism to the growth of the host root system, the overall shape of lateral root development, etc., and achieve low-cost effects

Inactive Publication Date: 2019-06-21
NORTHWEST A & F UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, neither test-tube nodulation nor spray cultivation can grasp the information of nodules on the host root growth's gravitropism, overall morphology, lateral root development, etc.
Therefore, there is still a lack of a convenient method for non-damaging, real-time and comprehensive monitoring of nodule and root growth and development under the condition of ensuring that the plant has a good growth state.

Method used

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  • Simple and effective method for non-destructive observation of root nodules and root morphology
  • Simple and effective method for non-destructive observation of root nodules and root morphology
  • Simple and effective method for non-destructive observation of root nodules and root morphology

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Embodiment Construction

[0014] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings, so as to further understand the purpose, working principle and functions of the present invention.

[0015] Seeds of Medicago truncatula are first soaked in 75% alcohol for 6-8 minutes, washed with distilled water for 3-5 times, scratched the seed coat along the back suture of the seed with a scalpel (be careful not to hurt the embryo), and then placed in two layers Petri dishes (150 mm) of filter paper moistened with sterile water. Wrap the petri dish with tinfoil, place it at 4°C for 72 hours and then culture it upside down at 22°C (ensure that there is a certain amount of water in the lid of the petri dish for seed germination). When the radicle grows to 4-5 cm (about 2-3 days) when transplanting, carry out water culture seedling ( figure 1 ,2). When Medicago truncatula grew to the 3-leaf stage (the third true leaf just emerged), it was t...

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Abstract

Real-time observation of root nodules and root morphology is of great significance to leguminous plant research, but at present, a leguminous plant culture system which is convenient for root observation, efficient in nodulation, suitable for growth, economical and practical does not exist. Herein, alfalfa is used as a plant material, and a paper bag hydroponic method capable of observing root nodules and root system states in real time is established.

Description

technical field [0001] The invention relates to a simple and effective method for observing root nodules and root system morphology without damage. Background technique [0002] The root system of leguminous plants can symbiosis with rhizobia to form a special structure of root nodules. In the root nodule symbiotic system, the host plant provides various nutrients for the rhizobia before the rhizobia start to fix nitrogen. The rhizobia converts N2 into NH4+ through nitrogenase, providing nitrogen sources for plants, especially in nitrogen-deficient soils. A large number of studies have shown that intercropping or crop rotation of crops and legumes can significantly improve soil nitrogen nutrition, and high yields can also be achieved under the condition of no or less nitrogen fertilizer application. Therefore, with the increasing advocacy of low-carbon agriculture, in addition to the nitrogen uptake, transport and utilization mechanism of plant roots in the soil, the symbio...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 刘金隆曾引伟张恒榜
Owner NORTHWEST A & F UNIV