Dominant serotype strains of a riemerella anatipestifer disease area and its application

A technology of Riemerella anatipestifer and Mergerella anatipestifer, applied in the directions of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of easy generation of drug resistance, food safety, drug residues, etc., and achieve easy promotion and pertinence. Strong, virulent effect

Active Publication Date: 2019-06-25
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although antibacterial drugs can quickly control the disease, it is easy to relapse after stopping the drug, and

Method used

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  • Dominant serotype strains of a riemerella anatipestifer disease area and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0029]An epidemiological survey was carried out on Riemerella anatipestifer to obtain the dominant serotype of Riemerella anatipestifer, and then 126 strains of Riemerella anatipestifer isolated from different areas in Hubei Province were analyzed by culture characteristics, Morphological characteristics, biochemical characteristics, serotype identification, virulence test and immunogenicity test, and two dominant serotype strains with strong pathogenicity and good immunogenicity were screened. The specific preparation method is as follows:

[0030] 1. Source of disease materials: Tissues with typical lesions such as pericarditis, perihepatitis, and air sacculitis that were submitted or collected from duck farms in Xinzhou, Hubei, clinically with neurological symptoms, and autopsyed.

[0031] 2. Main reagents: Tryptic Soy Agar (TSA) and Tryptic Soy Broth (TSB) were purchased from Difco Company; MacConkey Agar was purchased from Hangzhou Microbial Reagent Co., Ltd.; calf serum ...

Embodiment 2

[0038] Example 2 Application of regional dominant serotype strains of Riemerella anatipestifer

[0039] Inoculate suitable culture medium with Riemerella anatipestifer RAHB-1 and Riemerella anatipestifer RAHB-2, harvest the culture, inactivate it with formaldehyde, add aluminum hydroxide glue and mix to make aluminum hydroxide glue seedling. It is used to prevent Riemerella anatipestifer, that is, duck serositis.

[0040] 1. Preparation of bacterial solution for vaccine production: Inoculate Riemerella anatipestifer RAHB-1 and Riemerella anatipestifer RAHB-2 into TSB containing 5% calf serum respectively, and culture on a shaker at 37°C for 24-48 hours , mixed at a volume ratio of 1:1 to obtain a mixed bacterial solution. Sampling for pure inspection and counting of live bacteria, according to "People's Republic of China Veterinary Pharmacopoeia (2010 Edition)" appendix 42 for pure inspection, should be pure. Count live bacteria according to Appendix 19 of "The Veterinary Ph...

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Abstract

The invention belongs to the technical field of biological products, and particularly relates to two dominant serotype strains of a riemerella anatipestifer disease area and its application. The strains are riemerella anatipestifer RAHB-1 and riemerella anatipestifer RAHB-2, which are deposited in the China Center for Type Culture Collection (Wuhan University Depository Center). The riemerella anatipestifer RAHB-1 and riemerella anatipestifer RAHB-2 are subjected to inoculation culture to obtain bacteria liquid, the bacteria liquid can be mixed, and a vaccine used for preventing duck scrositisis prepared by formaldehyde inactivation and addition of hydrogenated alumina gel for mixing. The vaccine has high safety, low cost, good immune effect and easy absorption, provides basic guarantee for clinical application of the vaccine, and is easy to promote.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a regional dominant serotype strain of Riemerella anatipestifer and its application. Background technique [0002] Riemerella anatipestifer (RA) disease is an important waterfowl bacterial disease and one of the important diseases that seriously endanger the duck industry. It is caused by Riemerella anatipestifer in domestic ducks, turkeys, geese and other A contagious infectious disease of a variety of poultry, also known as duck infectious serositis, its main autopsy pathological changes are fibrinous pericarditis, perihepatitis, air sac inflammation, meningitis, caseous salpingitis, etc. Ducks of all ages can be infected, ducklings are the most susceptible, 2 to 6 weeks old are more frequent, the morbidity and mortality are higher, and often mixed with duck colibacillosis. With the increasing development and intensification of the duck industry, the oc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
Inventor 罗玲罗青平温国元邵华斌卢琴王红琳汪宏才张腾飞张蓉蓉张文婷商雨
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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