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Method and application of using salicylic acid to induce accumulation of flavonoids and terpene lactones in plant leaves

A technology for plant leaves and induced plants, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve problems such as unrealized industrialization, and achieve the effect of low cost and high content

Active Publication Date: 2021-08-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been experimental studies based on cell lines, the way to obtain EGB in vitro has not yet been industrialized

Method used

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  • Method and application of using salicylic acid to induce accumulation of flavonoids and terpene lactones in plant leaves
  • Method and application of using salicylic acid to induce accumulation of flavonoids and terpene lactones in plant leaves
  • Method and application of using salicylic acid to induce accumulation of flavonoids and terpene lactones in plant leaves

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Embodiment Construction

[0028] 1. Materials

[0029] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0030] 2. Method

[0031] 2.1 Configure salicylic acid solution

[0032] After dissolving the solid salicylic acid with absolute ethanol, add Tween20, and then dilute with distilled water, wherein the final volume fraction of ethanol is 2-5%, and the final volume fraction of Tween20 is 0.02-0.05%.

[0033] Configure 0-100mg·l according to the above method -1 Salicylic acid solutions with different concentration gradients were used for follow-up experiments to obtain salicylic acid solutions with four concentration gradients of 0mg / L (CK), 50mg / L (SA1), 80mg / L (SA2), and 100mg / L (SA3).

[0034] 2.2 Test treatment

[0035] The 10-year-old Dafo finger grafted ginkgo tree in Taixing City, Jiangsu Province was selected as...

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Abstract

The invention discloses a method and application for using salicylic acid to induce the accumulation of flavonoids and terpene lactones in plant leaves, belonging to the field of biotechnology, including using 50-100mg / L salicylic acid when the plant leaf cells are fully mature The acid solution is sprayed on the plant leaves for 1-2 times, and the treated plant leaves can be obtained by conventional methods to obtain ginkgo leaf extract with high active ingredients. Compared with the prior art, the method is simple, low in cost, pollution-free, stable and efficient, and the content of flavonoids and terpene lactones obtained after regulation and control by the method of the present invention is high, among which quercetin, kaempferol, iso Compared with the control, the contents of rhamnetin, total flavonoids, ginkgolide A, ginkgolide B and total terpene lactone increased by 23.37%, 19.96%, 22.18%, 18.90%, 9.34%, 25.56%, 11.24%, respectively. %.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and application of using salicylic acid to induce the accumulation of flavonoids and terpene lactones in plant leaves. Background technique [0002] Salicylic acid (Salicylic Acid, SA), the chemical name is o-hydroxybenzoic acid, phenolic acid substances, is a kind of signaling molecule and a new type of plant hormone ubiquitous in plants, and plays an important role in many physiological reactions and gene expression. control role. After the compound of salicylic acid enters the plant body, it forms salicylic acid through hydrolysis and exerts its effect. As a plant hormone related to stress resistance, salicylic acid can enter plant cells, regulate plant secondary metabolic pathways, and have an important impact on the synthesis and accumulation of plant secondary metabolites. When pathogens infect plants, endogenous SA can respond quickly and resist external stress by r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/40A01P21/00
CPCA01N37/40
Inventor 汪贵斌国颖冯宇
Owner NANJING FORESTRY UNIV