Bone repair material and application thereof

A bone repair and bone defect technology, applied in the field of medical biomaterials and medical devices, can solve the problems of poor mechanical properties and strong immunogenicity, and achieve the effects of convenient storage, safe and reliable products, and good mental state of animals

Active Publication Date: 2019-07-02
天新福(北京)医疗器材股份有限公司
View PDF7 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of strong immunogenicity and poor mechanical properties in the prior art, the present invention provides a bone repair material

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bone repair material and application thereof
  • Bone repair material and application thereof
  • Bone repair material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Evaluation of the phase composition and components of bone meal prepared from bovine bone

[0049] Select bovine bone samples (isolated cancellous bone) soaked in strong alkali, strong oxidant, hot water, degrease, deproteinize, decellularize, dehydrate and then calcined at high temperature to inactivate possible viruses, grind and sieve to adjust pH to neutral to get natural bone meal.

[0050] 1) The isolated cancellous bone comes from healthy cattle under 3 years old in China, and is cut into bone pieces with a side length of no more than 2cm, and the blood stains on the bone pieces are washed away.

[0051] 2) The purpose of alkalization is to degrease and decellularize, and to prepare lye: 1M NaOH solution.

[0052] Primary alkalization: the mass volume ratio of bone block to lye solution is 1:2, soak for 24 hours.

[0053] Impurity removal: peel off the impurities on the bone block.

[0054] Secondary alkalization: The mass volume ratio of bone block...

Embodiment 2

[0067] Embodiment 2: the preparation of bone repair material

[0068] 1. Preparation of natural hydroxyapatite: prepare natural hydroxyapatite (natural bone meal) according to the method described in Example 1;

[0069] 2. Preparation of collagen gel:

[0070] 1) Slowly add 500 ml of chondroitin sulfate acetic acid solution into 500 ml of collagen acetic acid swelling solution, the stirring speed is 15000-20000 rpm, the stirring time is 1-2 hours, and the solution is evacuated to obtain collagen-chondroitin sulfate slurry;

[0071] Chondroitin sulfate acetic acid solution configuration:

[0072] Add 2-4 grams of chondroitin sulfate into 500 milliliters of 0.4-0.6% acetic acid solution, dissolve at 0-10 DEG C, and filter to obtain.

[0073] Collagen acetic acid swelling solution configuration:

[0074] Add 3-5 grams of type I collagen to 500 ml of acetic acid solution with a concentration of 0.3-0.6%, mix and stir, the stirring speed is 15000-20000 rpm, the stirring tempera...

Embodiment 3

[0087] Example 3: Morphology and component analysis of bone repair materials and bone powder

[0088] The morphology of the bone repair material prepared in Example 2 was observed by a scanning electron microscope (SEM, S4800, HATICHI), and the element distribution was analyzed by an attached EDS.

[0089] image 3 It is the SEM photo of the bone repair material, from which it can be seen that the phosphate particles in the bone repair material are less than 250 μm, formed by the adhesion of collagen, the collagen and the phosphate particles are distributed alternately, the collagen is in the shape of a smooth film, and the phosphate particles further A large number of hydroxyapatite crystals of about 500nm can be observed by zooming in. A micro-nano-scale pore structure is formed between the nano-crystals. This structure is retained from the microstructure of natural bone, which makes the bone repair material have water absorption and adsorption to growth factors and other pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Porosityaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention provides a bone repair material. a molecular structure and a ratio of contents of calcium and phosphorus of the bone repair material are very similar to that of natural bone, and the bone repair material is provided with excellent biocompatibility. When the bone repair material is implanted into a bone defect, a fibrous tissue boundary surface does not exist between bone tissue and hydroxyapatite in the repair material, a bone-like apatite mineral material can be formed on a rear surface inside an implant body, and the bone repair material dynamically participates in bone tissueabsorption and reconstruction and a metabolic process of calcium ions and phosphonium ions. At the same time, the bone repair material of the has excellent osteoconductivity and osteoinductivity, a three-dimensional structure allows functions such as blood vessel ingrowth, cell penetration and adhesion, new bone formation, tissue deposition and calcification, and a bionic microstructure is beneficial to aggregation of bone morphogenetic protein (BMP), thereby generating the osteoinductivity.

Description

technical field [0001] The invention belongs to the fields of medical biological materials and medical instruments, and in particular relates to a bone repair material, a preparation method and an application thereof. Background technique [0002] For a long time, the repair of bone defects mainly adopts the method of autologous bone graft combined with allogeneic bone graft. Autologous bone has high osteoinductive activity and no immunogenicity, but the source of autologous bone is limited. Bone allografts have problems with eliciting an immune response in the body and may cause cross-infection. These bio-derived materials have inevitable defects, and synthetic or natural materials have become promising substitutes for bone defect repair materials. [0003] A good inducible artificial bone material should have the following characteristics: ①Good biocompatibility and biodegradability; ②Osteoconduction and combined with other active molecules to induce osteogenesis; ③Can l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/46A61L27/58A61L27/56
CPCA61L27/46A61L27/56A61L27/58A61L2430/02C08L89/00
Inventor 陈刚郭晓霖
Owner 天新福(北京)医疗器材股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap