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A selenium-enriched bacterium and its isolation method

A technology of enriching selenium and bacteria, applied in the field of microorganisms, can solve the problems of high production cost, poor stability, limited selenium enrichment ability, etc., and achieve the effect of simple operation and single variety

Active Publication Date: 2020-11-17
BEIJING UNION UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, most of the artificially cultivated strains have problems such as poor stability, limited selenium enrichment ability, and high production cost.

Method used

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  • A selenium-enriched bacterium and its isolation method
  • A selenium-enriched bacterium and its isolation method
  • A selenium-enriched bacterium and its isolation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The selenium-enriched bacteria isolated in the present invention come from the soil of Ankang farmland in Shaanxi.

[0034] 1. Enrichment

[0035] Prepare meat extract peptone medium and 90 mL of sterile water containing 10 g of glass beads, and sterilize at 121 °C for 20 min. After cooling to room temperature, take 10 g of the soil sample from Ankang, Shaanxi, add it to sterile water, and shake it well to make a soil suspension. Take 1 mL of the soil suspension and transfer it to 50 mL of meat extract peptone liquid medium in a shaker at 25°C, 100rmp Rotate speed enrichment culture for 24 hours to obtain a bacterial suspension for later use.

[0036] 2. Primary screening

[0037] After the enrichment culture, according to the volume of 5% bacterial liquid, use a sterilized pipette gun to take the enriched bacterial suspension and add it to the meat extract peptone liquid medium with a sodium selenite concentration of 100mg / L to continue the enrichment culture After ...

Embodiment 2

[0050] 1. Growth curve of selenium-enriched strain W41

[0051] Prepare several meat extract peptone liquid culture media respectively, mark them according to a certain time sequence, and place them in a shaker at a temperature of 25°C and a rotation speed of 100rmp for cultivation. According to the arranged time sequence, after taking out successively, measure the absorbance value at 600nm of UV-Vis spectrophotometer, which is used for drawing the growth curve of strain W41. The results are as follows: Figure 4 shown.

[0052] from Figure 4 It can be seen from the growth curve of the bacterial strain that the bacterial strain W41 belongs to the logarithmic growth phase from 4 hours to 48 hours, and the growth amount of the bacterial strain W41 continues to increase and grows rapidly. During this period, the metabolism of the bacterial strain is very vigorous and the transformation rate is high; It is in a relatively stable growth stage within 48 to 144 hours, and the grow...

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Abstract

The present invention provides a selenium-enriched bacterium and its isolation method. The scientific name of the selenium-enriched bacterium is Comamonas sp. W41, and the depository unit is: General Microbiology Center of China Microbiological Culture Collection Management Committee; Address: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, Chaoyang District; date of deposit: May 24, 2018, deposit registration number: CGMCC NO.15803. The strain has the characteristics of selenophilic microorganisms, and has potential application value in the biotransformation of selenium, the microbial synthesis of nano-selenium, and the remediation of selenium-polluted environments.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to a selenium-enriched bacterium, in particular to a selenium-enriched bacterium and a separation method thereof. Background technique [0002] Selenium (Se) is a trace element ubiquitous in nature, widely distributed in the crust, soil and lakes and other environments. Selenium pollution mainly comes from petroleum refining, electronics and photoelectric industries, glass, rubber and various alloy manufacturing industries. Selenium mainly exists in four valence states: negative divalent selenium, zero-valent selenium, tetravalent selenium, and hexavalent selenium. Among them, tetravalent selenium exists in the environment in the form of selenite. Because selenite is highly soluble, it is easy to transfer and transform in the environment. At the same time, selenite can inactivate proteins, hinder DNA repair, and interfere with the normal operation of cell respiration and antioxidant defe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 万鹰昕
Owner BEIJING UNION UNIVERSITY
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