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Selenium-rich bacterium and separation method thereof

A separation method and selenium enrichment technology, applied in the field of microorganisms, can solve the problems of high production cost, poor stability, limited selenium enrichment ability, etc., and achieve the effect of simple operation and single variety

Active Publication Date: 2019-07-05
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, most of the artificially cultivated strains have problems such as poor stability, limited selenium enrichment ability, and high production cost.

Method used

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  • Selenium-rich bacterium and separation method thereof
  • Selenium-rich bacterium and separation method thereof
  • Selenium-rich bacterium and separation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The selenium-enriched bacteria isolated in the present invention come from the soil of Ankang farmland in Shaanxi.

[0034] 1. Enrichment

[0035] Prepare meat extract peptone medium and 90 mL of sterile water containing 10 g of glass beads, and sterilize at 121 °C for 20 min. After cooling to room temperature, take 10 g of the soil sample from Ankang, Shaanxi, add it to sterile water, and shake it well to make a soil suspension. Take 1 mL of the soil suspension and transfer it to 50 mL of meat extract peptone liquid medium in a shaker at 25°C, 100rmp Rotate speed enrichment culture for 24 hours to obtain a bacterial suspension for later use.

[0036] 2. Primary screening

[0037] After the enrichment culture, according to the volume of 5% bacterial liquid, use a sterilized pipette gun to take the enriched bacterial suspension and add it to the meat extract peptone liquid medium with a sodium selenite concentration of 100mg / L to continue the enrichment culture After ...

Embodiment 2

[0050] 1. Growth curve of selenium-enriched strain W41

[0051] Prepare several meat extract peptone liquid culture media respectively, mark them according to a certain time sequence, and place them in a shaker at a temperature of 25°C and a rotation speed of 100rmp for cultivation. According to the arranged time sequence, after taking out successively, measure the absorbance value at 600nm of UV-Vis spectrophotometer, which is used for drawing the growth curve of strain W41. The results are as follows: Figure 4 shown.

[0052] from Figure 4 It can be seen from the growth curve of the bacterial strain that the bacterial strain W41 belongs to the logarithmic growth phase from 4 hours to 48 hours, and the growth amount of the bacterial strain W41 continues to increase and grows rapidly. During this period, the metabolism of the bacterial strain is very vigorous and the transformation rate is high; It is in a relatively stable growth stage within 48 to 144 hours, and the grow...

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Abstract

The invention provides a selenium-rich bacterium and a separation method thereof. The scientific name of the selenium-rich bacterium is Comamonas sp. W41, the Comamonas sp. W41 is preserved in China General Microbiological Culture Collection Center which is located in Institute of Microbiology of Chinese Academy of Sciences No. 3, No. 1 Courtyard, West Beichen Road, Chaoyang District, Beijing on May 24th, 2018, and the preservation registration number of the Comamonas sp. W41 is CGMCC No. 15803. The selenium-rich bacterium has the features of selenophilic microorganisms and has potential application values in selenium biological conversion, nano selenium microbial synthesis and the remediation of environments polluted by selenium.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to a selenium-enriched bacterium, in particular to a selenium-enriched bacterium and a separation method thereof. Background technique [0002] Selenium (Se) is a trace element ubiquitous in nature, widely distributed in the crust, soil and lakes and other environments. Selenium pollution mainly comes from petroleum refining, electronics and photoelectric industries, glass, rubber and various alloy manufacturing industries. Selenium mainly exists in four valence states: negative divalent selenium, zero-valent selenium, tetravalent selenium, and hexavalent selenium. Among them, tetravalent selenium exists in the environment in the form of selenite. Because selenite is highly soluble, it is easy to transfer and transform in the environment. At the same time, selenite can inactivate proteins, hinder DNA repair, and interfere with the normal operation of cell respiration and antioxidant defe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 万鹰昕
Owner BEIJING UNION UNIVERSITY
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