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Application of ganoderma lucidum polysaccharide in freezing long-term storage of livestock semen

A technology of Ganoderma lucidum polysaccharide and cryopreservation, which is applied in the fields of application, preservation of human or animal body, animal husbandry, etc., can solve problems such as low survival rate, and achieve the effect of reliable effect, easy promotion and maintenance of fertilization ability.

Inactive Publication Date: 2019-07-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies in the prior art, the present invention provides a cryoprotectant for livestock semen and its application in cryopreservation of pig, sheep and cattle semen, which solves the problem of low viability of livestock sperm in the prior art after freezing-thawing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of pig semen cryoprotectant of the present invention comprises the steps:

[0022] Step 1, prepare the frozen base solution: accurately measure 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate, dissolve them in 100mL double-distilled water, adjust the pH to 6.21, and adjust the osmotic pressure 286mosm / L, prepared as frozen base fluid;

[0023] Step 2: Accurately measure 80 mL of frozen base liquid, add 20 mL of fresh egg yolk, 0.030 mg l-0.060 mg of ganoderma polysaccharide, and mix well to obtain a cryopreservative for livestock semen (I liquid) of the present invention.

[0024] Take 94mL of the above-mentioned livestock semen cryoprotectant and add 6mL glycerin, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservative (II solution) of the present invention for storing pig semen, and put it in a 4°C refrigerator Reserved for the follo...

Embodiment 2

[0045] The preparation method of goat semen cryopreservation agent of the present invention, comprises the steps:

[0046] Step 1, preparing the frozen base liquid, the method is the same as in Example 1.

[0047] Step 2: Accurately measure 85 mL of the frozen base solution, add 15 mL of fresh egg yolk and 0.020 mg-0.085 mg of Ganoderma lucidum polysaccharide, and mix well to prepare a livestock semen cryopreservative (I liquid) of the present invention.

[0048] Get 88mL of the above-mentioned livestock semen cryopreservation agent and add 12mL glycerol to be mixed with 100mL of mixed solution, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservation agent (II liquid) of the present invention for preserving sheep semen, Put it in a refrigerator at 4°C for the following tests.

[0049] (1) Semen collection

[0050] The semen is collected by the pseudo-vaginal method, and the routine quality inspection is carried out at 34-37°C wit...

Embodiment 3

[0067] The preparation method of bovine semen cryopreservation agent of the present invention, comprises the steps:

[0068] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0069] Step 2: Accurately measure 80 mL of the frozen base solution, add 20 mL of fresh egg yolk and 0.040 mg-0.100 mg of ganoderma polysaccharide to obtain a livestock semen cryopreservative (I liquid) of the present invention.

[0070] Add 6 mL of glycerin to the above-mentioned livestock semen cryopreservation agent, mix well, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservation agent (II solution) of the present invention for storing bovine semen, and put it in 4°C Store in the refrigerator for the following experiments.

[0071] (1) Semen collection

[0072] Semen was collected by the pseudo-vaginal method, and routine quality inspection was carried out at 37°C with a microscope. Select semen with no peculiar smell, mil...

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PUM

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Abstract

The invention discloses a protective agent applied to frozen storage of livestock semen. The protective agent can remarkably improve the survival rate of livestock sperms, and the main substance of the protective agent is ganoderma lucidum polysaccharide. The preparation method is mainly characterized in that the ganoderma lucidum polysaccharide is used as the protective agent for long-term preservation of straw frozen semen of pigs, sheep and cattle, for artificial insemination, and for insemination after long-distance transportation of the semen subjected to ultralow temperature refrigeration for the first time at home and abroad. The survival rate, the acrosome integrity and plasma membrane integrity of sperms of pigs, sheep and cattle after freezing and thawing are notably improved.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and in particular relates to the application of ganoderma lucidum polysaccharide as an antifreeze protective agent in cryopreservation of livestock semen. Background technique [0002] Sperm freezing technology is an important innovation in artificial insemination. It not only solves the problem of long-term preservation of male animal semen, but also facilitates the preservation of genetic resources. It is also convenient to apply and is not limited by time and space. , which plays an important role in establishing a global gene bank of excellent species. The wide application of sperm freezing technology is conducive to improving the utilization rate of excellent breeding males, reducing the number of breeding males, reducing economic costs, and improving the efficiency of animal husbandry, especially in the field of artificial insemination. Play...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李青旺吴琳刘定帮
Owner NORTHWEST A & F UNIV
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