A New Method for Evaluating the Quality of Sperm

Abstract

The invention discloses a novel sperm quality evaluation method. The present invention uses a large-field microscopic imaging system to realize the large-field multi-target tracking and positioning detection of live sperm samples, and uses a high-resolution microscopic imaging system to realize high-resolution morphological imaging of live sperm samples. The combination of two microscopic imaging systems Through the rapid scanning of the imaging area of ​​the high-resolution microscopic imaging system in the large-field imaging area, all the selected target live sperm in the large-field imaging area are subjected to high-resolution morphology imaging, and finally the viability and High-resolution morphology detection is especially suitable for high-resolution morphology analysis of a large number of motile sperm in a large field of view in human semen examination. It can simultaneously detect indicators such as sperm motility and morphology of unstained and unlabeled semen samples.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging for semen examination, and in particular relates to a novel sperm quality evaluation method. Background technique [0002] As a key part of human semen examination, sperm motility and morphology are of great significance to reproductive and developmental research, especially to clinical reproductive medicine. Existing medical clinical testing uses different sample preparation and microscopic detection methods to detect the concentration, vitality and morphology of sperm respectively. [0003] Sperm motility can be done manually or computer-aided. Using computer-aided sperm analysis technology can provide quantitative parameters of sperm motility with high accuracy, which is conducive to the standardization and standardization of semen examination. Computer-aided sperm analysis technology uses low-magnification microscopic imaging system, image acquisition, processing and analysis syst...

AI Technical Summary

Problems solved by technology

In addition, since the sperm motility detection and morphological analysis are performed separately, it is impossible to obtain the corresponding relationship between sperm motility and morphology, which are crucial for sperm quality analysis

[0004] The movement speed of sperm is extremely fast, with a speed of tens of microns / second. Large-field imaging is required to ensure continuous observation of moving sperm and hundreds of target tracking; while the head size of sperm is 3 to 5 microns, and the morphological structure such as vesicles is in the On the order of hundreds of nanometers, while the limit resolution of low-magnification imaging is at the micron level, clear imaging cannot be achieved, and the shape of the head cannot be accurately measured and analyzed

[0005] Limited by the existing optical imaging design, the field of view of imaging is inversely proportional to the magnification of the objective lens. Low magnification imaging can achieve a large field of view but cannot achieve high resolution. High magnification imaging can achieve high resolution and cannot directly achieve a large field of view.

Although for non-moving samples, large field of view and high resolution imaging can be performed by methods such as imaging field of view stitching, but for fast moving objects such as sperm, simple field of view stitching is not suitable, which will cause the movement of sperm. omission or duplication

The effective imaging area is limited by the microscopic imaging detection element and optical design at the same time, and the effective imaging area of ​​the microscopic image acquisition element is limited by technology and cost

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