A New Sperm Quality Evaluation System

Abstract

The invention discloses a novel sperm quality evaluation system. The present invention realizes the multi-target detection of the sample with a large field of view by using a large-field microscopic imaging system, and realizes the high-resolution microscopic imaging of the sample by using a high-resolution microscopic imaging system, and the combination of two microscopic imaging systems realizes a large field of view for the sample High-resolution imaging detection is especially suitable for high-resolution morphology analysis of a large number of motile sperm in a large field of view in human semen examination. It is a system that can simultaneously detect the concentration, motility, and morphology of live sperm in non-stained and unlabeled semen samples.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging for semen examination, and in particular relates to a novel sperm quality evaluation system. Background technique [0002] As a key part of human semen examination, sperm motility and morphology are of great significance to reproductive and developmental research, especially to clinical reproductive medicine. Existing medical clinical testing uses different sample preparation and microscopic detection methods to detect the concentration, vitality and morphology of sperm respectively. [0003] Sperm motility can be done manually or computer-aided. Using computer-aided sperm analysis technology can provide quantitative parameters of sperm motility with high accuracy, which is conducive to the standardization and standardization of semen examination. Computer-aided sperm analysis technology uses low-magnification microscopic imaging system, image acquisition, processing and analysis syst...

AI Technical Summary

Problems solved by technology

[0004] The movement speed of sperm is extremely fast, the speed is tens of microns per second, and a large field of view microscopic imaging is required to ensure continuous observation of moving sperm and hundreds of target tracking; and the size of the head of sperm is 3 to 5 microns, and the low magnification The limit resolution of magnified imaging is above the micron level, and high-resolution microscopic imaging cannot be achieved, and the shape of the head cannot be accurately measured and analyzed

[0005] Limited by the existing optical imaging design, the imaging field of view is inversely proportional to the magnification of the objective lens. Low magnification imaging can achieve a large field of view but cannot achieve high resolution. High magnification imaging can achieve high resolution and cannot directly achieve a large field of view. Through splicing Such methods are suitable for non-moving samples, but not suitable for moving objects such as sperm

The effective imaging area is limited by both the microscopic imaging detection element and the optical design, and the effective imaging area of ​​the microscopic image acquisition element is limited by technology and cost

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