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Application of amlexanox in the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury

A radiation-induced lung injury and ionizing radiation technology, applied in the field of medicine, can solve unseen problems and achieve the effects of less toxic side effects, inhibiting radiation-induced lung injury, and broad application prospects

Inactive Publication Date: 2019-07-16
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on the prevention and treatment of amlexanox in radiation-induced lung injury in the prior art

Method used

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  • Application of amlexanox in the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury
  • Application of amlexanox in the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury
  • Application of amlexanox in the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Cell culture: RLE-6TN and MLE 12 cells were cultured in DMEM / F12 medium containing 10% fetal bovine serum. All cells were placed at 37°C, 5% CO 2 Cultured in an incubator, subcultured every 2-3 days, and the cells in the logarithmic growth phase were used for experiments.

[0034] (2) CCK-8 colorimetric method: RLE-6TN and MLE 12 cells (1*10 4 / mL) were inoculated into 96-well plates, and 5 parallel wells were set up for each concentration. Different concentrations of amlexanox were added to continue culturing for 48 hours. CCK-8 reagent was added to the cell culture medium at a ratio of 1:100 to continue culturing for 1-4 hours. The absorbance at 450 nm was measured with a microplate reader. Finally, the formula of cell survival rate=dosage group value / control group value×100% was used to calculate the cell survival rate.

[0035] The result is as figure 1 As shown, amlexanox less than or equal to 50 uM has no inhibitory effect on the growth and proliferation...

Embodiment 2

[0037] (1) Cell culture is the same as in Example 1;

[0038] (2) The CCK-8 colorimetric method is the same as in Example 1.

[0039] The result is as figure 2 As shown, the cell growth and proliferation levels of the administration group were significantly higher than those of the control group, and the 25uM amlexanox treatment without toxic side effects was better than the 6.5uM administration treatment.

Embodiment 3

[0041] (1) Cell culture is the same as in Example 1;

[0042] (2) Cell clone formation method: Take RLE-6TN and MLE 12 cells in the logarithmic growth phase, and inoculate different numbers of cells into six-well plates according to different irradiation dose requirements (0, 2, 4, 6 Gy doses of inoculated cells are respectively for 200, 400, 800 and 1600 pcs). After 24 hours, 1 hour before irradiation, 25uM amlexanox was added for treatment, and the same amount of DMSO was used as a negative control in the control group. Two groups of cells received different doses (0, 2, 4, 6Gy) at the same time 60 Co gamma rays were irradiated, and culture was continued for 4 days after irradiation. Both groups were replaced with ordinary medium, and culture was continued for 10-14 days, until cell clones visible to the naked eye appeared in the culture dish, and the culture was terminated. The medium was discarded, washed twice with PBS, fixed with 4% paraformaldehyde for 30 minutes, sta...

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PUM

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Abstract

The invention relates to the field of new medical uses, and particularly relates to an application of amlexanox in the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury. The structure formula of the amlexanox is as follows. According to the invention, the provided amlexanox for the preparation of drugs for the prevention and treatment of ionizing radiation-induced radioactive lung injury: is small in toxic and side effects, and is widely used in the clinical treatment of recurrent oral ulcer, asthma, allergic rhinitis, etc.; and the amlexanox is remarkable in curative effect, and can significantly inhibit radiation-induced lung injury when given at a dose of 50 mg / kg / day three days before irradiation. It shows that the amlexanox isunique in the prevention and protection of ionizing radiation-induced radioactive lung injury, and has broad application prospects in the medical domain in China.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new application of amlexanox, that is, an application in the preparation of medicines for preventing and treating radiation-induced lung injury caused by ionizing radiation. Background technique [0002] Radiation therapy is widely used clinically as an important treatment for thoracic malignant tumors. Chest radiotherapy often causes a serious complication—radiation lung injury, including early radiation pneumonitis and late radiation pulmonary fibrosis. Eventually lead to respiratory failure. The occurrence of radiation lung injury greatly reduces the therapeutic effect and survival rate of patients receiving chest irradiation. Therefore, the protection and treatment of radiation-induced lung injury caused by radiotherapy for thoracic tumors has extremely important medical significance. Currently, there is no effective treatment except for symptomatic treatment with gluco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/436A61P11/00A61P17/16
CPCA61K31/436A61P11/00A61P17/16
Inventor 曲红金高福杨彦勇蔡建明刘蕾曹堃廖泽彬刘哲陈媛媛雷霄
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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