Establishment method and application of central post-stroke pain (CPSP) animal model

A technology for establishing methods and animal models, which can be used in biochemical equipment and methods, medical preparations containing active ingredients, pharmaceutical formulations, etc. , There has not been a good construction of CPSP animal model and other problems, to achieve the effect of high success rate, simple method, fast and repeatable

Active Publication Date: 2019-07-16
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

"Study on Type VII Collagenase-Induced Rat Cerebral Hemorrhage Model" (Ni Houjie et al., Nerve Injury and Functional Reconstruction 2012, 7(6): 411-412) disclosed a type VII collagenase-induced cerebral hemorrhage model, which was successful , but the neurobehaviour of this model is affected, and it is not the same as the clinical symptoms of CPSP, so it cannot be used to study the pathogenesis of CPSP and screen effective drugs. There is no good method for constructing an animal model of CPSP.

Method used

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  • Establishment method and application of central post-stroke pain (CPSP) animal model
  • Establishment method and application of central post-stroke pain (CPSP) animal model
  • Establishment method and application of central post-stroke pain (CPSP) animal model

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Embodiment 1

[0045] The method for establishing the CPSP animal model in this embodiment includes: weighing the animal, and fixing it on a stereotaxic device after isochlorane anesthesia. Under the guidance of a stereotaxic instrument, inject (0.2U / 0.5μl physiological saline) type VII collagenase (sigma) into the VPL of the right thalamic ventroposterolateral nucleus (anterior fontanelle as the origin, positioned 3.8mm behind the anterior fontanelle, sideways 3.3mm, 6.0mm deep under the skull surface), the injection position is as figure 2 As shown in -B, the ellipse is the injection site, the injection speed is 0.05μl / min, and the control group is injected with the same amount of saline. After the injection, keep the needle for 5 minutes to prevent collagenase from overflowing, and then slowly withdraw it to prevent backflow along the needle tube. Intraoperative animal temperature was maintained with a rectal thermometer coupled with a heating blanket. After the operation, the patient wi...

Embodiment 2

[0049] In the method for establishing a CPSP animal model in this example, the total amount of injected type VII collagenase was 0.1 U, and the injection volume was 0.4 μL, and the rest were the same as in Example 1.

[0050] In the method for establishing a CPSP animal model in this embodiment, the construction success rate is 60%.

Embodiment 3

[0052] In the method for establishing a CPSP animal model in this example, the total amount of injected type VII collagenase was 0.3 U, and the injection volume was 0.6 μL, and the rest were the same as in Example 1.

[0053] In the method for establishing a CPSP animal model in this embodiment, the construction success rate is 75%.

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Abstract

The invention relates to an establishment method and an application of a central post-stroke pain (CPSP) animal model and belongs to the technical field of animal model construction. The establishmentmethod comprises the step of injecting 0.1-0.3 U of VII-type collagenase into a right thalamic ventroposterior lateral nucleus of rat brain to obtain the CPSP animal model. With the adoption of the thalamic brain pain model established by stereo-position injection of VII-type collagenase into the dorsal thalamic ventroposterior lateral nucleus of a rat, pain characteristics of nervus centralis pathological thalamus pain are evaluated by detecting mechanical pain, heat pain, cold pain and biped balance pain; the emotional response of anxiety and forced swimming are evaluated by field experiments, and the depression state is evaluated by sweet water experiment; the occurrence and change condition of neuroinflammation reaction is evaluated by immunofluorescence and immunoblotting; a transmission electron microscope analyzes the ultrastructure change, screens the CPSP animal model and evaluates the neurobiology mechanism of behavioral features and occurrence and development of cerebral hemorrhage thalamus pain after stereo-position injection of VII-type collagenase.

Description

Technical field [0001] The invention relates to a method and application for establishing a CPSP animal model, and belongs to the technical field of animal model construction. Background technique [0002] Spontaneous intracerebral hemorrhage (ICH) has the characteristics of high morbidity, high mortality and high disability rate. It accounts for 15%-20% of strokes and the mortality rate is as high as 40%. Thalamic hemorrhage accounts for about 13% to 30% of cerebral hemorrhage. According to reports, at least 11-32% of stroke patients have post-stroke pain (Central post-stroke pain, CPSP). CPSP pain is one of the common symptoms of stroke patients, and it is the most unbearable, stubborn, and difficult to treat. disease. Studies have shown that CPSP has a higher proportion of bulbar infarction and thalamic hemorrhage. The ventral posterior nucleus and dorsal nucleus of the thalamus account for 3.2% and 25%, respectively. The VPL of the posterior thalamic nucleus plays a vital r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K49/00
CPCA61K38/4886C12Y304/24A61K49/0008
Inventor 陈雪梅王建李玉萍臧卫东王君敏贾培君任秀花邢寅霈周淑娅张嘉欣石小雨陈丹阳李文博
Owner ZHENGZHOU UNIV
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