A kind of mulberry proteoglycan, its preparation method and application

A technology of proteoglycan and polysaccharide, applied in the field of mulberry proteoglycan, its preparation and application

Active Publication Date: 2021-05-28
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the role of morula proteoglycan in the treatment of Alzheimer's disease.

Method used

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  • A kind of mulberry proteoglycan, its preparation method and application
  • A kind of mulberry proteoglycan, its preparation method and application
  • A kind of mulberry proteoglycan, its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The preparation of embodiment 1 morula proteoglycan FMP-6-S4

[0054] a. Proteoglycan extraction:

[0055] 1 kg of dried mulberries was pulverized by a pulverizer, and 20 L of deionized water was added. At 55 °C, 30 g of cellulase, 10 g of amylase and 5 g of papain were added for extraction for 1 hour, and the temperature was raised to 100 °C to inactivate the enzyme, and centrifuged , the filtrate was concentrated and dialyzed against flowing water for 3 days. Heat and concentrate the dialyzed internal solution to 3L, centrifuge to discard the precipitate, add five times the volume (15L) of 95% v / v ethanol to the supernatant under stirring, let it stand for alcohol precipitation overnight, and centrifuge, and the obtained precipitate is washed with anhydrous Alternately washed with ethanol and acetone for 3 times, the precipitate was collected by centrifugation and vacuum-dried at 50° C. to obtain 152 g of crude proteoglycan from mulberry fruit (yield: 15.2%).

[005...

Embodiment 2

[0071] Example 2. Proteoglycan FMP-6-S4 inhibits Aβ 42 generation

[0072] 1. Aβ in CHO / APPBACE1 cells and HEK 293-APPsw cells 42 ELISA detection

[0073] CHO / APPBACE1 cells (from Shanghai Institute of Materia Medica, Chinese Academy of Sciences) were cultured in Ham's F12 medium (purchased from American Hyclone Company), HEK293-APP sw Cells (donated by Zhang Haiyan's research group at Shanghai Institute of Materia Medica, Chinese Academy of Sciences) were cultured in DMEM high-glucose medium containing 10% fetal bovine serum, 100 U / mL penicillin, 100 μg / mL streptomycin and 200 μg / mL G418. When the cells grow to 80%-90% confluence, 5×10 5 The density of cells / well was seeded in a 24-well plate in 5% CO 2 , After culturing in a 37°C incubator for 24 hours, add mulberry polysaccharide FMP configured at different concentrations (0 μg / mL, 31.25 μg / mL, 62.5 μg / mL, 125 μg / mL, 250 μg / mL, 500 μg / mL and 1000 μg / mL) -6-S4 (prepared in Example 1), after 24 hours, the cell supernata...

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Abstract

The present invention provides a kind of proteoglycan FMP-6-S4 containing galacturonan obtained from mulberry fruit (Fructus Mori), its preparation method, and described proteoglycan FMP-6-S4 in the preparation prevention and / or treatment of neurodegenerative diseases, inhibition of Aβ 42 Generation or aggregation or use in drugs or health products for Alzheimer's disease. The proteoglycan FMP‑6‑S4 comprises 78% to 82% by weight polysaccharide and 18% to 22% by weight protein; the polysaccharide is composed of galacturonic acid, galacturonic acid Lactose, arabinose, rhamnose and glucose. The proteoglycan FMP-6-S4 has a potential therapeutic effect on Alzheimer's disease, and is expected to be developed as a drug for treating Alzheimer's disease.

Description

technical field [0001] The present invention relates to proteoglycans, their preparation methods and their use in the preparation of medicines, more specifically, to a proteoglycan FMP-containing galacturonan extracted from mulberry fruit (Fructus Mori). 6-S4, its preparation method, and the proteoglycan FMP-6-S4 in the preparation of prevention and / or treatment of neurodegenerative diseases, inhibition of Aβ 42 Generation or aggregation and use in Alzheimer's disease medicines or health products. Background technique [0002] Alzheimer's disease (AD), also known as Alzheimer's disease, is a progressive neurodegenerative disease. The main clinical manifestations of AD are gradual loss of memory, cognitive dysfunction, abnormal behavior and social impairment. wait. With the increasing aging of the population, the incidence of AD is also increasing year by year, and it has become one of the most important health issues of public concern. [0003] The deposition and abnormal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K38/16A61P25/28
CPCA61K38/168C08B37/0003C08B37/006
Inventor 丁侃李赛娟廖文锋
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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