A microbial preparation for degrading vomitoxin and its application
A technology of DON and microorganisms, applied in the directions of microorganisms, microorganisms, microorganism-based methods, etc., can solve problems such as unsatisfactory detoxification effect and introduction of other harmful substances
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Example 1 Sample Collection, Strain Isolation and Screening
[0025] 1. Sample collection time and place
[0026] On the morning of May 5, 2017, soil samples of peanut fields were collected from the experimental base of Shandong Peanut Research Institute (Wangcheng Town, Laixi City, Qingdao City).
[0027] 2. Sample processing, strain isolation and screening
[0028] Take 1 g of the collected soil sample and suspend it in 10 mL of sterile water in an ultra-clean bench, shake and dilute to prepare a soil suspension, and then dilute 100 times with sterile distilled water. Take 100 μL of the suspension and spread it on the LB solid plate, and place it at 37°C for cultivation. After 3 days, the grown colony is streaked and purified on the LB solid plate. After 3 times of streaking and purification, a strain is obtained, numbered A2025 .
Embodiment 2
[0029] Identification of embodiment 2 bacterial strain A2025
[0030] According to the method described in "Berger Bacteria Identification Handbook" (Eighth Edition), the morphological characteristics and physiological and biochemical characteristics of strain A2025 were identified, and the specific results were as follows:
[0031] 1. Morphological characteristics: The single colony of strain A2025 is raised on LB medium, milky white, opaque ( figure 1 ), after 2 days of culture at 37°C, the colony was about 4-6mm, and wrinkles appeared on the surface of the colony after 3 days of culture.
[0032] 2. Physiological and biochemical characteristics: Oxidase activity is negative, can grow at 4-54°C, Gram staining is positive, can hydrolyze and utilize casein, gelatin and starch, but cannot utilize Tween 40 and Tween 80.
[0033] 3. 16S rRNA gene analysis
[0034] The bacterial genomic DNA of A2025 was extracted, and the 16S rRNA gene general primer was used for PCR amplificati...
Embodiment 3
[0036] Example 3 Degradation of Bacillus siamese A2025 to vomitoxin
[0037] 1. Cultivation of Bacillus siamese A2025 degrading vomitoxin
[0038] The culture medium of Bacillus siamese A2025 for degrading vomitoxin is TSB medium: 15g tryptone, 5g soybean peptone, 5g sodium chloride, 1000mL water, pH 7.2.
[0039] Take out the frozen Bacillus siamica A2025 from the -80°C refrigerator, inoculate 50 μL of the Bacillus siamica A2025 bacterial solution into a 5 mL TSB liquid medium test tube in the ultra-clean workbench, and cultivate the activated strain for 12 hours. Inject 500 μL of activated bacterial liquid into 100 mL of TSB liquid medium, shake and culture at 37 °C for 96 h, and the concentration of the bacterial liquid is 2.1×10 8 cfu / mL.
[0040] 2. Degradation of vomitoxin by Bacillus siamese A2025
[0041] Take 1.98mL of Bacillus siamese A2025 bacteria solution (bacterial solution concentration 2.1×10 8 cfu / mL) into a 10mL sample tube, add 20μL of 200ppm vomitoxin w...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More