Ultra-trace multi-biological macromolecule detecting method

A biomacromolecule and detection method technology, applied in the field of ultra-trace multiplex biomacromolecule detection, can solve the problems of difficulty in sample recovery, high cost, inconvenient operation, etc., and achieve the effects of improving detection sensitivity, low cost, and easy washing

Active Publication Date: 2019-07-26
SOUTHEAST UNIV
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  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: The present invention provides a detection method for ultra-trace multivariate biomacromolecules for the problems of inconvenient operation, high cost and difficulty in sample recovery of current technical methods , which uses the difference in surface wettability and natural evaporation to drive concentration to achieve the concentration of the detection target to achieve the effect of ultra-sensitive detection

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Embodiment 1

[0034] A method for detecting ultra-trace multivariate biomacromolecules, comprising the following steps:

[0035] (1) Preparation of silica single emulsion:

[0036] The discrete phase for preparing the single emulsion is an aqueous silica solution, and the mass-volume ratio is 20%. The continuous phase is methyl silicone oil with a viscosity of 50cs. The flow rate of the discrete phase is 0.5mL / h, and the flow rate of the continuous phase is 3mL / h. In a microfluidic chip, inertial and viscous forces work together to shear the discrete phase into a single emulsion. The single emulsion was then collected into a methicone collection pool filled with 500 cs.

[0037] (2) Single emulsion curing:

[0038] Transfer the collection tank containing the single emulsion to an oven at 80 degrees Celsius and let it stand for 12 hours to partially volatilize the water in the emulsion, then soak the collection tank with n-hexane for 20 minutes to remove the silicone oil, and then put th...

Embodiment 2

[0046] A method for detecting ultra-trace multivariate biomacromolecules, comprising the following steps:

[0047] (1) Preparation of monodisperse polystyrene emulsion:

[0048] The discrete phase for preparing the single emulsion is polystyrene (PS) / benzene / dichloroethane, and the mass volume ratio is 0.5g:10mL:10mL. The external phase is an aqueous solution of polyvinyl alcohol (PVA), and the mass volume ratio is 5%. Using a stable liquid flow rate (inward flow rate 0.5mL / h) and shear force (outward flow rate 5mL / h), the water phase of PVA cuts the oil phase of PS into an oil-in-water monoemulsion structure with a consistent diameter, and then the emulsion is collected by The tube goes into a collection tank filled with the PVA aqueous phase. As a surfactant, the presence of PVA can effectively prevent the fusion of emulsions during the collection process;

[0049] (2) Curing of polystyrene emulsion:

[0050] Transfer the prepared emulsion droplet to the flask of the rota...

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Abstract

The invention discloses an ultra-trace multi-biological macromolecule detecting method based on a mixed hydrogel magnetic photonic crystal barcode. The mixed hydrogel magnetic photonic crystal barcodeis obtained by utilizing a protein structure assembled by a monodisperse microsphere prepared by a microfluidic device as a template, then pouring mixed magnetic nanoparticle hydrogel into a templateand removing the template. Then a probe is coupled on the surface of the mixed hydrogel photonic crystal barcode and then integrated into the super-hydrophobic surface, the difference in wettabilityis utilized to achieve natural concentration and enrichment of a sample to achieve the purpose of ultra-trace biological macromolecule detection. Based on the stable optical characteristics such as the characteristic reflection peak of the photonic crystal, detection of a plurality of biological macromolecules, that is, multivariate detection can be achieved simultaneously. In summary, the concentrated enrichment method has the advantages of simple operation, low cost, high sensitivity and high versatility, and the method can be applied to the detection of clinical ultra-sensitive multi-microbial macromolecules.

Description

technical field [0001] The invention relates to a biomacromolecule detection method, in particular to an ultra-trace multivariate biomacromolecule detection method based on a hybrid hydrogel magnetic photonic crystal barcode and utilizing natural evaporation to drive concentration. Background technique [0002] Biomacromolecules (such as nucleic acids, proteins, etc.) are important components of the human body. Characterization of human biomacromolecules is an important strategy for clinical diagnosis, DNA research, bacterial / viral infection, drug development, mutation analysis, and gene therapy. However, the small size and high dilution of biomacromolecules present in human blood seriously affect the accuracy and sensitivity in the detection process of biomacromolecules. How to achieve ultrasensitive multiple detection of biomacromolecules at low density is an important obstacle restricting the development of detection technology. [0003] At present, there are many signa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6816
CPCC12Q1/6816
Inventor 赵远锦蔡丽均卞非卡
Owner SOUTHEAST UNIV
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