Method for determining abamectin in soil

A technology of abamectin and soil, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem of few soil detection methods, and achieve good application prospects, improve efficiency, and optimize detection conditions.

Inactive Publication Date: 2019-07-26
SHANGHAI SEP ANALYTICAL SERVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are fewer reports on the existence and detection of abamecti

Method used

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  • Method for determining abamectin in soil
  • Method for determining abamectin in soil
  • Method for determining abamectin in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:

[0032] A. Accurately weigh 20.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...

Embodiment 2

[0050] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:

[0051] A. Accurately weigh 15.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...

Embodiment 3

[0054] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:

[0055] A. Accurately weigh 25.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...

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Abstract

The invention relates to a method for determining abamectin in soil, which comprises the following steps: adding a soil sample into acetone for treatment, and extracting a sample solution to be detected; preparing an abamectin standard solution by using acetonitrile as a solvent; respectively detecting and analyzing the sample solution to be detected and the abamectin standard solution by adoptinga high performance liquid chromatography; and carrying out qualitative and quantitative analysis on the abamectin B1a and the abamectin B1b in the soil sample according to the detection and analysisresults. The method for determining abamectin in soil provided by the invention has the advantages of simple and convenient detection process, high sensitivity and low detection limit, which can realize the rapid detection of the abamectin content in an actual soil sample, and has good application prospect.

Description

technical field [0001] The invention relates to a method for determining abamectin in soil. Background technique [0002] Avermectin is a class of 16-membered macrolide compound with insecticidal, acaricidal and nematicidal activities, which is produced by fermentation of Streptomyces avermitilis in Streptomyces griseus. Because it has a good control effect on pests of various crops such as vegetables, fruit trees, cotton, tobacco, soybeans, and tea trees, and can delay drug resistance, abamectin pesticides have already begun to be used on a large scale. Natural avermectin contains 8 components, mainly 4 kinds, namely A1a, A2a, B1a and B2a, the total content of which is ≥80%; the corresponding 4 homologues with smaller proportions are A1b, A2b, B1b and B2b , its total content ≤ 20%. Currently commercially available avermectin pesticides use Abamectin (i.e. Avermectin B1a+B1b, wherein B1a is not less than 90% and B1b is not more than 5%) as the main insecticidal component. ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/14G01N30/08
CPCG01N30/06G01N30/08G01N30/14G01N30/88G01N2030/047G01N2030/062G01N2030/146G01N2030/884
Inventor 朱洋
Owner SHANGHAI SEP ANALYTICAL SERVICES CO LTD
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