Method for determining abamectin in soil
A technology of abamectin and soil, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem of few soil detection methods, and achieve good application prospects, improve efficiency, and optimize detection conditions.
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Embodiment 1
[0031] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:
[0032] A. Accurately weigh 20.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...
Embodiment 2
[0050] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:
[0051] A. Accurately weigh 15.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...
Embodiment 3
[0054] For realizing above object, realize the method for measuring Abamectin in soil by following technical scheme, it is characterized in that, comprise the following steps:
[0055] A. Accurately weigh 25.0g of soil sample, accurate to 0.001g, into a 250mL glass bottle, add 50mL of acetone to shake and extract for 1h, wash and filter the filtered residue with 10mL of acetone and repeat three times, and collect the solution obtained after four times of filtration together to obtain a soil extract. A sufficient amount of anhydrous sodium sulfate was added to the soil extract for dehydration, and then the dehydrated soil extract was concentrated to 2 mL using a KD concentrator to obtain a concentrated extract. First use 5ml of methanol, then use 5ml of ultrapure water to activate the SPE C18 column; completely transfer the concentrated extract into the activated SPE C18 column, then rinse with 5mL of ultrapure water, discard the eluent and use 5mL Methanol was used for elutio...
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