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Method for harvesting microalgae and obtaining microalgae biomass

A biomass and microalgae technology, applied in microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of high energy consumption for microalgae harvesting, difficulty in recycling aquaculture water, etc., and achieve green harvesting. , energy saving, strong stability effect

Pending Publication Date: 2019-07-30
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for harvesting microalgae and obtaining microalgae biomass with reduced energy consumption in order to overcome the problems of high energy consumption in microalgae harvesting and difficulty in recycling aquaculture water existing in the prior art

Method used

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  • Method for harvesting microalgae and obtaining microalgae biomass
  • Method for harvesting microalgae and obtaining microalgae biomass
  • Method for harvesting microalgae and obtaining microalgae biomass

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Embodiment approach

[0024] It is well known to those skilled in the art that nitrogen sources (and other nutrients—especially carbon sources) can be added to the culture system in order to obtain higher amounts of microalgal biomass. Therefore, according to a preferred embodiment of the present invention, the method also includes monitoring the nitrogen source (such as NO) in the culture system during the cultivation process. 3 - and / or NO 2 - ) content, so that nitrogen sources (such as NO 3 - and / or NO 2 - ). When nitric acid is used as nitrogen source, the amount of added nitric acid is such that the pH value of the culture system is maintained in the range of 6.5-10 (preferably 7-9.5). The supplementary amount of carbon source (such as glucose) usually makes the content of carbon source in the culture system in the range of 5-10g / L.

[0025] According to a specific embodiment, the method may also include removing NO in the flue gas x converted to NO 3 - and / or NO 2 - , and use it...

Embodiment 1

[0044] Chlorella species were purchased from the Institute of Hydrobiology, Chinese Academy of Sciences and cultured on BG11 medium. The algae were induced for 2 days in the BG11 medium containing 5g / L glucose, and then inserted into a 5L light fermenter containing 3L BG11 medium for aseptic adjunct culture. After inoculation, the algae liquid OD 680 Control at about 1, culture conditions are: temperature 28°C, ventilation rate about 0.9vvm, stirring speed about 280rpm, light intensity 6000lux, light-dark cycle 12h:12h, adding antibiotics to control bacterial growth (kanamycin 50mg / L and chlorine Mycin 10mg / L), and add 0.1% by volume of antifoaming agent (emulsified silicone oil). During the culturing process, the cell density (based on the absorbance OD at 680nm 680 as an indicator) to monitor, and supplement nutrients according to the growth status of microalgae, so as to control the glucose concentration at 5-10g / L, and the additional nitrogen source is HNO 3 , HNO 3 The...

Embodiment 2

[0051] Scenedesmus was purchased from the Institute of Hydrobiology, Chinese Academy of Sciences and cultured on BG11 medium. Add 3LBG11 medium into the 5L Erlenmeyer flask, then inoculate, OD after inoculation 680 The control is about 0.8, and the breeding conditions are: temperature 28°C, ventilation rate 0.8-1vvm, light intensity 6000lux, light-dark cycle 12h:12h, during the breeding process, the cell density (absorbance OD at 680nm 680 as an indicator) to monitor and supplement HNO 3 and other nutrient salts, the pH is controlled between 7-8, and 80% by volume of the algae liquid (the content of microalgae in terms of dry weight is 1.2g / L) is taken out after 7 days of cultivation for membrane filtration. The inlet pressure of the membrane filtration unit is 0.1MPa, the outlet pressure is 0.05MPa, and the molecular weight cut-off of the membrane is 30kDa. The concentrated solution after membrane filtration (the content of microalgae in terms of dry weight is 87g / L) is spr...

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Abstract

The invention relates to the field of microalgae, and discloses a method for harvesting microalgae and obtaining microalgae biomass. The method for harvesting the microalgae comprises the step that membrane filtration equipment is used for concentrating a harvested microalgae culture solution; in the step, the harvested microalgae culture solution is circulated in a loop provided with the membranefiltration equipment, when the microalgae culture solution flows through a filter membrane in the membrane filtration equipment, part of liquid in the microalgae culture solution permeates through the filter membrane, and a permeating solution and a concentrated algae solution are obtained respectively at the two sides of the filter membrane. The invention further discloses a method for obtainingthe microalgae biomass. The method for obtaining the microalgae biomass comprises the steps of microalgae culture, microalgae harvesting and microalgae biomass extraction, and is characterized in that the microalgae is harvested by adopting the method. According to the technical scheme, the method for harvesting the microalgae and obtaining the microalgae biomass has the advantages that the microalgae can be effectively harvested, the stability is strong, the energy consumption is reduced to a great extent, the generation amount of wastewater is reduced, and green harvesting is achieved.

Description

technical field [0001] The invention relates to the field of microalgae harvesting, in particular to a method for harvesting microalgae and obtaining microalgae biomass. Background technique [0002] "Energy" and "environment" are important issues facing human society in the sustainable development of the new century. On the one hand, the fossil energy supporting modern human civilization is non-renewable, so countries around the world are stepping up the development of alternative energy technologies; on the other hand On the one hand, when humans process and use fossil energy, they will inevitably produce serious waste gas and sewage discharge problems, which have already caused serious impacts on the climate and human living environment. These problems require a coordinated solution. [0003] Microalgae are highly efficient "living chemical factories" driven by sunlight. Through the efficient photosynthesis of microalgae cells, light energy is converted into chemical ene...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02C12P1/00C12R1/89
CPCC12N1/02C12N1/12C12P1/00C12N1/125C12R2001/89
Inventor 朱俊英荣峻峰纪洪波宗保宁李煦程琳黄绪耕周旭华
Owner CHINA PETROLEUM & CHEM CORP
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