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Lactobacillus plantarum DP189 and application thereof

A technology of Lactobacillus plantarum and DP189, applied in the direction of Lactobacillus, application, bacteria, etc., can solve problems such as unclear mechanism, achieve broad application value, increase abundance, and reduce content

Active Publication Date: 2019-07-30
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that probiotics can reduce or prevent the development of intestinal diseases, such as diarrhea, inflammatory bowel disease, irritable bowel syndrome, etc., and 40-50% of patients with irritable bowel syndrome are accompanied by mental disorders such as anxiety and depression. Symptoms, some studies have shown that after the intervention of probiotics in patients with irritable bowel syndrome, the depression behavior is reduced while the intestinal discomfort is relieved, and the depression mood is improved, but the mechanism is still unclear

Method used

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  • Lactobacillus plantarum DP189 and application thereof
  • Lactobacillus plantarum DP189 and application thereof
  • Lactobacillus plantarum DP189 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0078] The preparation method of fluoxetine hydrochloride solution: get the granule in the fluoxetine hydrochloride capsule, dissolve with 0.5g / 100ml sodium carboxymethylcellulose aqueous solution. The dose of fluoxetine hydrochloride is calculated as particulate matter.

[0079] The preparation method of the corticosterone solution: take the corticosterone powder, add 0.1% DMSO and 0.1% Tween, and dissolve it to a dose of 40 mg / kg with physiological saline.

Embodiment 1

[0080] Embodiment 1, isolation, identification and preservation of plantarum lactobacillus strain

[0081] 1. Isolation of Lactobacillus plantarum strains

[0082] In December 2017, fresh pickled sauerkraut samples were collected from Tonghua City, Jilin Province, put into transport medium, and quickly placed in an ice box and sent to the laboratory for strain isolation. After the sauerkraut sample was ground and serially diluted, the Lactobacillus in the sample was isolated using a Lactobacillus selective medium, cultured at 37°C for 48 hours, and typical colonies on the plate were picked (milky white, round, with neat edges, raised, Opaque, wet and smooth surface), streak culture MRS solid medium, and isolate pure colonies. The purely cultured strains were inoculated into liquid MRS medium for culture, then added with 60% glycerol, and stored in a -80°C refrigerator. One of the Lactobacillus strains was named DP189.

[0083] 2. Identification of strains

[0084] Physiolo...

Embodiment 2

[0090] Example 2. Protection of Lactobacillus plantarum DP189 on hippocampal neurotrophic pathway and inhibition of hippocampal neuron apoptosis

[0091] 1. Preparation of bacterial suspension

[0092] 1. Lactobacillus plantarum DP189 (hereinafter referred to as DP189) was inoculated in liquid MRS medium, cultured at 37°C for 16 hours, centrifuged at 4°C at 6000rpm for 8 minutes, and the bacterial precipitate was collected.

[0093] 2. Take the bacterial precipitate obtained in step 1 and suspend it with sterile physiological saline to obtain a bacterial concentration of 2×10 10 CFU / mL of DP189 bacterial suspension.

[0094] Two, group processing

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Abstract

The invention discloses lactobacillus plantarum DP189 and application thereof, and relates to the field of functional food microorganisms. The strain is preserved in China Center for Type Culture Collection on March 25 , 2019 with the preservation number of CCTCC NO:M2019199. By means of the strain, the learning and memory capability of a depressed rat can be improved; the level of brain-derived neurotrophic factors of the depressed rat can be improved; the injury to hippocampal neurons and apoptosis of neuron cells of the depressed rat are reduced; the content of apoptosis proteins JUK2 and Bcl-2 of brain tissue of the depressed rat is lowered; the abundance of verrucomicrobiaceae of the intestinal tract is lowered; the abundance of bacterium lacticum of the intestinal tract is improved, the alpha-diversity of the intestinal floras is improved, and the intestinal flora disturbance of the depressed rat is alleviated. In this way, the lactobacillus plantarum DP189 has good effects of preventing and treating depression / anxiety, infantile autism and inflammatory enteritis. The strain has the anti-depression function, and can serve as a functional probiotic bacterium for preventingand treating the depression, infantile autism and inflammatory enteritis, and a new idea is provided for clinical intervention with and control over depression and other relevant mental diseases.

Description

technical field [0001] The invention relates to the technical field of functional food microorganisms, in particular to a plant lactobacillus DP189 and its application. Background technique [0002] According to data released by the World Health Organization in 2017, the number of people affected by depression in the world has increased by 18% in the past 10 years. Depression has become an important disease that plagues human living standards and causes social and economic burdens around the world. With the rapid development of economy in our country, the rhythm of people's life and work has been significantly accelerated, and the accompanying psychological and mental pressure has generally increased, leading to an upward trend in the prevalence of mental disorders. According to statistics from the Ministry of Health, the prevalence of depressive disorder in my country is 3.59%, and more than 80% of depressive patients have not received standardized treatment, resulting in r...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P25/24A61P1/00A23L33/135C12R1/25
CPCA61K35/747A61P25/24A23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2200/322
Inventor 李盛钰赵玉娟段翠翠赵子健王超高磊杨舸
Owner JILIN ACAD OF AGRI SCI
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