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Paenibacillus kribbensis and preparation and application thereof

A technology of bacillus and microbial preparations, applied in the direction of application, fungicides, bacteria, etc., to achieve the effect of promoting the growth of tobacco plants, increasing yield and output value

Active Publication Date: 2019-07-30
漳州三炬生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on Paenibacillus mainly focuses on the breeding of excellent strains and improving the production of active substances by means of fermentation or genetic engineering, while for the purification of active substances of Paenibacillus kribbensis metabolites , identification and application in the prevention and treatment of plant fungal diseases still have great deficiencies and research space

Method used

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  • Paenibacillus kribbensis and preparation and application thereof
  • Paenibacillus kribbensis and preparation and application thereof
  • Paenibacillus kribbensis and preparation and application thereof

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preparation example Construction

[0027] The invention provides a microbial preparation containing Paenibacillus cribbus, and the preparation method of the microbial preparation comprises the following steps:

[0028] (1) activating the above-mentioned Paenibacillus cribbus with beef extract peptone medium to obtain a single colony or a lawn;

[0029] (2) Inoculate the single bacterial colony or bacterial lawn into the liquid Zaburi medium, shake and culture at 28-32°C, 150-250r / min for 36-48 hours, to obtain the propagation solution;

[0030] (3) Inoculate the multiplication solution into a liquid fermentation medium, and ferment and cultivate at 28-30° C. for 4-5 days to obtain a microbial preparation containing Paenibacillus cribbus.

[0031] In the present invention, the above-mentioned Paenibacillus cribbus is firstly activated with beef extract peptone medium to obtain a single colony or a lawn. The present invention does not specifically limit the source and specific composition of the beef extract pep...

Embodiment 1

[0042] (1) Isolation of Paenibacillus sp.

[0043] Paenibacillus crib was isolated from the rhizosphere soil of tobacco planting in Henan. Methods as below:

[0044] Collect rhizosphere soil samples from tobacco planting areas in Henan, weigh 10.0g of soil, put it into a conical flask with 100mL sterile water, place the conical flask on a shaker at 220r / min for 5min; -1 ~10 -6 Diluted rhizosphere soil suspension; draw 100μL of 10 -4 ~10 -6 Soil suspensions with different dilutions were evenly spread on the beef extract peptone medium plate with aseptic technique, and 3 to 5 parallels were made for each dilution, and then placed in a constant temperature incubator at 28-30°C for 2-2 5d. After the colonies grow out, pick a single colony and transfer it to the beef extract peptone medium for purification to obtain pure culture. Using Phytophthora nicotiana, Fusarium and other pathogenic fungi as indicator bacteria, the primary screening of antagonistic bacteria was carried ...

Embodiment 2

[0062] Determination of the antibacterial activity of Paenibacillus by Cribb: plate confrontation method was used, and the pathogenic fungi tested were Phytophthora nicotiana and Fusarium.

[0063] After activating the antagonistic strains, spread and inoculate them on a plate and cultivate them for 24 hours, use a sterilized hole puncher with a diameter of 6mm to make a bacterial dish under aseptic conditions, then inoculate them around the PDA solid medium, and use sterilized The pathogenic bacteria were also made into a plate with a hole punch with a diameter of 6mm and inoculated in the middle of the PDA medium plate, and the treatment of only receiving the plate of the pathogenic bacteria was used as a control. Cultivate at a constant temperature of 28°C. When the control plate is covered with the whole plate, observe the results of confrontation culture. When the zone of inhibition no longer changes, record the size of the zone of inhibition and calculate the rate of inhi...

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Abstract

The invention provides a strain of paenibacillus kribbensis and a preparation and application thereof and belongs to the technical field of agricultural production and biocontrol microbial technology.The preservation number of the paenibacillus kribbensis is CGMCC No.17248. The provided paenibacillus kribbensis has a remarkable bacteriostatic activity on phytophthora nicotianae and fusarium, andthe bacteriostatic rates on phytophthora nicotianae and fusarium both exceed 70%. By applying the provided paenibacillus kribbensis to planting of tobacco, the growth of tobacco plants can be significantly promoted, and the yield and output value of the tobacco are increased.

Description

technical field [0001] The invention belongs to the technical field of agricultural production and biological control microorganisms, and in particular relates to a strain of Paenibacillus cribsii and its preparation and application. Background technique [0002] Plant diseases are factors that affect plant yields in modern agricultural production. According to FAO data, the annual losses caused by plant diseases in the world account for 10% to 15% of the total crop production. With the improvement of the agricultural planting industrial structure Adjustment, the control and prevention of crop diseases have attracted more and more attention. For a long time, most of the pesticides used to prevent and control plant diseases are chemical fungicides, and there are only a single variety of fungicides, and plant pathogenic bacteria have developed serious resistance to many varieties. In addition, due to the long-term and large-scale use of a few fungicides, the accumulation of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/01
CPCA01G13/00A01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 刘玉珍郭小红黄娟朱春苗骆毛喜林克明李肖宇余劲聪
Owner 漳州三炬生物技术有限公司
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