A Rapid Isolation Method of Intact Gut of Plutella xylostella larvae

Abstract

The invention provides a method for quickly separating a complete intestinal tract of plutella xylostella larva and belongs to the field of arthropod anatomy. According to the invention, without damage to plutella xylostella epidermis and other tissues, an insect scalpel is used for cutting a small opening on tail, a point of strength is found, the complete intestinal tissue of plutella xylostellalarva is quickly taken out, and the completeness of the intestinal tract of plutella xylostella larva can be extremely guaranteed. Compared with a traditional dissection method, the method provided by the invention has the advantages that the dissection time is greatly reduced, the whole dissection process only requires 30-60s, the pollution and waste of materials are reduced, degradation of tissues is avoided and an excellent basic experimental material is provided for subsequent research on intestinal tract tissue specificity, enteric microorganism, and the like.

Description

technical field [0001] The invention relates to the technical field of arthropod anatomy, in particular to a method for rapidly separating the complete intestinal tract of diamondback moth larvae. Background technique [0002] Plutella xylostella belongs to the silk insects of Lepidoptera, Insecta, Arthropoda, and the first hatched larvae are dark brown, and then turn green. The last instar larvae are spindle-shaped, with obvious body segments and black bristles on the epidermis. There are fat body, silk gland, midriff, Malpighian duct and other tissues in the abdomen. [0003] Plutella xylostella xylostella larvae are pests of cruciferous plants. They are widely distributed and cause great harm to agricultural production. Using their intestines as research materials is of great significance to the pest control of cruciferous crops and the development of new biopesticides. [0004] Dissection of Plutella xylostella larvae is an important technique to study its internal str...

AI Technical Summary

Problems solved by technology

However, the larvae of Plutella xylostella xylostella are small in size, and the body length of the last instar larvae is about 10-12 mm. Due to the slender and fragile intestines, conventional body surface dissection methods can easily cause mechanical damage to the intestines, resulting in leakage of intestinal contents. Exhaust, contaminate other tissues in the body, and waste a lot of time, and the long-term dissection operation will cause the degradation of the sample tissue material

[0005] After searching the existing literature at home and abroad, there is no report on the method of rapidly separating the intestinal tract of diamondback moth larvae

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