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A kind of microbial corrosion inhibitor and using method thereof

A technology of microbial corrosion and inhibitors, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as environmental protection and biological health damage, and achieve low toxicity effects

Active Publication Date: 2022-02-11
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term and large-scale use not only makes the bacteria highly resistant to drugs, but also causes great damage to environmental protection and biological health.

Method used

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  • A kind of microbial corrosion inhibitor and using method thereof
  • A kind of microbial corrosion inhibitor and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The microorganisms of the present embodiment are copper green pseudomonoosa: mccc1a00099 (Xiamen, China);

[0036] The medium used in this embodiment is 2216E medium, and the ingredient is: NaH 2 PO 4 5.0g / l, NA 2 SO 4 1.8G / L, CACL 2 0.55g / L, KCL 0.16g / L, NA 2 CO 3 0.08g / L, KBR 0.034G / L, SRCL 2 0.08g / L, SRBR 2 0.022g / L, H 3 BO 3 0.004g / L, Nasio 3 0.0024G / L, NAF 0.0016g / L, NH 4 NO 3 0.008g / L, protein 胨 1.0 g / L, iron citrate 0.1 g / L, yeast extract 0.1 g / L.

[0037] 1. Antibacterial experiment:

[0038] Minimal Antibacterial Concentration (MIC) and minimum bactericidal concentration (MBC) are an important indicator for measuring antibacterial properties. The present invention assays the value of the MIC and MBC of the paternal tea prepared by a trace dilution method. The results showed that children with tea-filled water were non-toxic to MC3T3-E1 cells; children with tea finish for 10 4 ~ 10 7 CFU ml -1 The minimum antibacterial concentration of ...

Embodiment 2

[0050] The desulfurization arms (China Ordinary Microbial Square Savings Center, CGMCC) in this embodiment.

[0051] The medium components employed in this embodiment are: MgSO 4 5g / L, NA 3 C 6 Hide 5 O 7 12.5g / L, CASO 4 2.5g / L, NH 4 CL 2.5g / L, K 2 HPO 4 2.5g / L, NAC 3 Hide 5 O 3 8.75g / L, yeast extract 2.5g / l, 20ml 5% (wt%) (NH) 4 ) 2 Fe (SO 4 ) 2 .

[0052] 1. Antibacterial experiment:

[0053] Minimal Antibacterial Concentration (MIC) and minimum bactericidal concentration (MBC) are an important indicator for measuring antibacterial properties. The value of the MIC and MBC of desulfurous disulfurus was determined using a micro-dilution method. The results show: Child tea mixture for 10 4 ~ 10 7 CFU ml -1 The minimum antibacterial concentration of the desulfurization arci bacteria is 100-300 ppm, the minimum bactericidal concentration is 500-1000 ppm, where:

[0054] Child tea mixture for 10 4 CFU ml -1 The minimum antibacterial concentration of the desulfurization a...

Embodiment 3

[0063] Bacillus (China Ordinary Microbial Square Safety Management Center, CGMCC) in this embodiment.

[0064] The basic medium used in this embodiment is used: sucrose 10g / L, K 2 HPO 4 13.9g / L, K 2 HPO 4 2.7g / l, NaCl 1g / L, yeast extract 1g / L, nano 3 1G / L, MGSO 4 0.25g / l. 10ml of trace element solution; trace element solution ingredient: MnCl 2 · 4h 2 O 18mg / L, CoCl 2 · 6h 2 O 27mg / L, H 3 BO 3 5.0mg / L, Cucl 2 · 2h 2 O 2.4mg / L, Namoo 4 · 2h 2 O 2.3mg / L, ZnCl 2 1.9mg / L.

[0065] 1. Antibacterial experiment:

[0066] Minimal Antibacterial Concentration (MIC) and minimum bactericidal concentration (MBC) are an important indicator for measuring antibacterial properties. Determination of the value of the Micheliostea of ​​Juji Bacillus was determined by micro dilution method. The results show: Child tea mixture for 10 4 ~ 10 7 CFU ml -1 The minimum bacteriobacterium concentration of the bacillus subtilum is 50-200 ppm, the minimum bactericidal concentration is ...

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Abstract

The present invention relates to a microbial corrosion inhibitor and its application method, wherein the inhibitor is a catechin hydrate solution with a concentration of 50-1000ppm. The method of use is as follows: prepare a catechin hydrate solution with a catechin hydrate solute concentration of 50-1000ppm, add inhibitors to microbial corrosion materials, and cultivate for 14 days to complete corrosion inhibition. The microbial strains Concentration is 10 4 ~10 7 CFU mL ‑1 . The catechin hydrate of the present invention can exert obvious and effective antibacterial, anti-biofilm and anti-corrosion properties on various bacteria at a low concentration of 50-1000 ppm. In addition, catechin hydrate has extremely low toxicity as a microbial inhibitor, and can be used as a green and environmentally friendly fungicide and corrosion inhibitor.

Description

Technical field: [0001] The present invention belongs to the technical field, and more particularly to a microbial corrosion inhibitor and a method of use. Background technique: [0002] Microbiologically Influenced Corrosion, MIC is a process of accelerating material corrosion directly or indirectly from the life activities of microorganisms and their metabolites. According to statistics, China's annual corrosion costs have been as high as 20 billion yuan. In 2014, my country's corrosion costs account for 3.34% of GDP. In various corrosive environments, in particular marine environments, the formation of material surface biofilms is one of the main causes of microbial corrosion. The physicochemical environment of the material under the biofilm changes, such as dissolved oxygen, ion concentration, pH, etc., and accompanied by the catalytic process of the microorganism to the electrochemical reaction, it ultimately leads to severe corrosion of the material. Therefore, in the rever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P1/00C23F11/12
CPCA01N43/16C23F11/12
Inventor 雅瑟莱克巴赫徐大可索姆亚埃尔阿比德萨阿德本苏达库雷希董雨侨李中王福会
Owner NORTHEASTERN UNIV LIAONING