Preparation process of holboellia latifolia and chaenomeles speciosa compound enzyme

Abstract

The invention discloses a preparation process of holboellia latifolia and chaenomeles speciosa compound enzyme. The preparation process comprises the following steps: (1), washing holboellia latifoliacleanly; taking fruit flesh; grinding the fruit flesh to be pulp; filtrating the pulp to obtain holboellia latifolia juice; washing chaenomeles speciosa cleanly; peeling and deseeding the chaenomelesspeciosa; grinding the chaenomeles speciosa without seeds into pulp; filtering the pulp to obtain chaenomeles speciosa juice; mixing the holboellia latifolia juice with the chaenomeles speciosa juiceto obtain a product A; (2), putting the product A into a fermentation tank, firstly adding white sugars into the fermentation tank, then adding saccharomycetes into the fermentation tank, and sealinga mouth of the fermentation tank and carrying out primary fermentation at a constant temperature to obtain a product B; and (3), adding lactobacillus into the product B, sealing the mouth of the fermentation tank and carrying out secondary fermentation at the constant temperature, and carrying out filtration to take a clear solution after fermentation is carried out completely so as to obtain holboellia latifolia enzyme. The holboellia latifolia enzyme prepared by adopting the process disclosed by the invention has the characteristics of higher content of ursolic acid, total phenols, total flavonoids and SOD (Super Oxide Dismutase), and higher DPPH (1,1-dipheny-2-picryhydrazyl) free radical scavenging ability, hydroxyl free radical scavenging ability and ABTS (2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging ability.

Description

technical field [0001] The present invention relates to a kind of preparation technology of enzyme, especially a kind of preparation technology of August melon and Xuan papaya composite enzyme, Background technique [0002] August melon is the fruit of Akebiatrifoliata (Akebiatrifoliata (Thunb.) Koidz.), also known as August Fried and Akebia. It is a natural green fruit with both medicine and food. People have been eating the wild fruit for a long time. Its pulp is sweet and delicious, delicious and juicy, and it is a rare delicacy. August melon is rich in a variety of soluble fructose, vitamins, macroelements and trace elements needed by the human body and other nutrients. It also has flavonoids, ursolic acid, and other medicinal and health-care ingredients. According to the life experience of the Tujia people in Zhangjiajie, the whole body of August melon can be used as medicine, and it is also a health fruit for beauty and health. [0003] Enzymes are mainly produced by...

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Problems solved by technology

[0005] Based on this, the researchers of the present application have carried out in-depth research on the production of August melon enzymes, and found that it is really difficult for the beneficial bacteria in the traditional fermentation process to ferment smoothly during the fermentation process. Therefore, the inventor added white sugar externally. method to increase the sugar content of the matrix, which solved the problem of the smooth fermentation of beneficial bacteria. However, after the addition of white sugar, a new problem occurred. Due to the high activity of beneficial bacteria, the degradation ratio of ursolic acid was greatly increased, resulting in the loss of bearberry in the enzyme. The acid content is reduced, which reduces the quality of August melon enzymes

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