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Ensiling method of sugarcane waste leaves

A technology for sugarcane tail leaves and sugarcane tails is applied in the field of silage of sugarcane tail leaves, which can solve the problems of insufficient utilization of the nutritional value of silage quality and the like.

Active Publication Date: 2019-08-09
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many disclosures on the treatment methods of sugarcane tail leaves, but the nutritional value of sugarcane tail leaves silage quality has not been efficiently utilized

Method used

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  • Ensiling method of sugarcane waste leaves
  • Ensiling method of sugarcane waste leaves
  • Ensiling method of sugarcane waste leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 Aspergillus oryzae (Aspergillus oryzae) XMS01 strain acquisition and naming:

[0029] The present invention is to obtain a mutant strain of Aspergillus oryzae after carrying out mutagenesis to a starting strain of Aspergillus oryzae preserved in the laboratory, which has been identified and preserved through microbial taxonomy: named Aspergillus oryzae (Aspergillusoryzae) XMS01, CCTCC NO: M 2018425; the preservation place is: Wuhan University, Wuhan, China; the China Collection Center for Type Cultures, and the preservation time is July 02, 2018.

[0030] The source of Aspergillus oryzae in this laboratory: the starting strain is a strain of Aspergillus oryzae preserved in our laboratory, the xylanase activity in the fermentation supernatant of the starting strain Aspergillus oryzae is 40.2u / mL, and the β-glucanase activity 12.4u / mL, cellulase activity 16.3u / mL.

[0031] The morphological characteristics of Aspergillus oryzae (Aspergillus oryzae) XMS01 with high-yiel...

Embodiment 2

[0062] A kind of silage method of sugarcane tail leaf, concrete steps are as follows:

[0063] The first step: Aspergillus oryzae (Aspergillus oryzae) XMS01 needs to prepare the inoculum before the aerobic silage fermentation treatment. The specific method is: use the high-salt Caspian medium to activate the plane medium, and then use the MRS medium to configure it. After high-temperature sterilization for 30 minutes, cool to room temperature, inoculate Aspergillus oryzae (Aspergillusoryzae) XMS01 by 3% inoculum amount, place in an incubator after mixing and cultivate for 2 days, and the temperature is controlled at 37° C.;

[0064] Lactobacillus plantarum (Lactobacillus plantarum) XMS02 and said plant Lactobacillus (Lactobacillus plantarum) XMS03MRS need to carry out inoculum preparation before carrying out anaerobic silage fermentation treatment. The specific method is: use high-salt Caspian culture medium to carry out plane medium activation treatment , then use the MRS med...

Embodiment 3

[0072] A kind of silage method of sugarcane tail leaf, concrete steps are as follows:

[0073] The first step: Aspergillus oryzae (Aspergillus oryzae) XMS01 needs to prepare the inoculum before the aerobic silage fermentation treatment. The specific method is: use the high-salt Caspian medium to activate the plane medium, and then use the MRS medium to configure it. After high-temperature sterilization for 30 minutes, cool to room temperature, inoculate Aspergillus oryzae (Aspergillusoryzae) XMS01 by 3% inoculation amount, place in an incubator after mixing and cultivate for 2.5 days, and the temperature is controlled at 37° C.;

[0074] Lactobacillus plantarum (Lactobacillus plantarum) XMS02 and said plant Lactobacillus (Lactobacillus plantarum) XMS03MRS need to carry out inoculum preparation before carrying out anaerobic silage fermentation treatment. The specific method is: use high-salt Caspian culture medium to carry out plane medium activation treatment , then use the MR...

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Abstract

The invention belongs to the field of microorganisms, and particularly relates to an ensiling method of sugarcane waste leaves. The ensiling method comprises the steps of 1, aerobic ensiling fermentation treatment and 2, anaerobic ensiling fermentation treatment, wherein for aerobic ensiling fermentation treatment in S1, the fresh sugarcane waste leaves are subjected to aerobic fermentation for 1-5 days through aspergillus oryzae XMS01, and for anaerobic ensiling fermentation treatment in S2, the sugarcane waste leaves subjected to the aerobic ensiling fermentation treatment are subjected to anaerobic ensiling fermentation treatment for 38-48 days through lactobacillus plantarum XMS02 and lactobacillus plantarum XMS03 to obtain the sugarcane waste leaves. The ensiling method has the advantages that in the first stage, the aspergillus oryzae (XMS01) is sprayed for aerobic fermentation, various non-starch polysaccharide enzymes accumulate, and the moisture content of wilted ensiling rawmaterials is reduced; in the second stage, the lactobacillus plantarum (XMS02) and the lactobacillus plantarum (XMS03) are sprayed for anaerobic ensiling, the fermentation speed is increased, and thepH of the ensiling feed is lowered; meanwhile, the enzymes accumulating in the aerobic fermentation stage are utilized, and thus the purpose of improving the ensiling quality of the sugarcane waste leaves is achieved.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a silage method for sugarcane tail leaves. Background technique [0002] Sugarcane is a grass plant and a common temperate and tropical economic crop. Sugarcane has been planted on a large scale in Guangxi, and the planting area has reached 14 million mu. Sugarcane tail leaves are the waste after sugarcane harvesting, generally accounting for 12-21% of sugarcane plant weight, but the utilization rate is less than 20% of the output. In the tropical areas of our country, there is a shortage of feed in winter. Reasonable storage and processing of sugarcane tail leaves can effectively solve the problem of insufficient forage and feed in the tropical areas of our country, and promote the development of animal husbandry in the tropical areas. [0003] Silage is an economical and convenient way to preserve roughage. Generally, the nutritional value of roughage after silage is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K30/18C12N1/14C12N1/20C12P1/02C12P1/04C12R1/69C12R1/25
CPCA23K30/18C12N1/14C12N1/20C12P1/02C12P1/04C12R2001/25C12N1/205C12R2001/69C12N1/145Y02P60/87
Inventor 何仁春周俊华王启芝邹彩霞周志扬罗鲜青黄丽霞梁琪妹黄香唐承明
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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