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Culture medium for algae seed culture expansion at pond mouth and culture expansion method

A technology of culture medium and algae species, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of slow onset of action, achieve reduced entrainment, simple expansion methods, and avoid algae The effect of settlement

Inactive Publication Date: 2019-08-09
无锡中顺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Farmers pay more and more attention to the cultivation of algae. Generally, they will add beneficial algae species such as Cyclotella to the pond before releasing the seedlings, and use fertilizers to promote the growth of beneficial algae. However, often because the beneficial algae directly put into the pond The amount is too small, the effect is too slow, sometimes it will cultivate some harmful algae such as moss in the pond

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of expansion method of pond mouth expansion culture algal species of the present invention, adopts following process steps:

[0019] Step 1, medium preparation: take 45 parts of sodium nitrate, 15 parts of potassium dihydrogen phosphate, 10 parts of magnesium sulfate, 10 parts of sodium carbonate, 5 parts of citric acid, 5 parts of sodium silicate, 5 parts of boric acid, and 5 parts of manganese chloride part; the above-mentioned raw materials were stirred and mixed for 40 minutes through a twin-shaft paddle mixer at normal temperature, and the rotating speed was 80 revolutions per minute, so as to obtain the culture medium for expanding and cultivating algae species at the pond mouth, and set aside;

[0020] Step 2, remove residual chlorine: take a 50L clean empty bucket, fill it with tap water, and explode for 24 hours to remove residual chlorine;

[0021] Step 3, preparation of the mixed bacterial solution: add 0.1kg of the culture medium for exp...

Embodiment 2

[0033] Embodiment 2: a kind of expansion method of pond mouth expansion cultivation algal species of the present invention adopts following process steps:

[0034] Step 1, medium preparation: take 50 parts of sodium nitrate, 10 parts of potassium dihydrogen phosphate, 8 parts of magnesium sulfate, 20 parts of sodium carbonate, 2 parts of citric acid, 2 parts of sodium silicate, 4 parts of boric acid, 4 parts of manganese chloride part; above-mentioned raw material is stirred and mixed 60 minutes through twin-shaft paddle mixer at normal temperature, and rotating speed is 70 revs / min, obtains the culture medium of pond mouth expansion cultivation algae, for subsequent use;

[0035] Step 2, remove residual chlorine: take a 70L clean empty barrel, fill it with tap water, and blast for 28 hours to remove residual chlorine;

[0036] Step 3, preparation of mixed bacterial solution: Add 0.2kg of the above-mentioned culture medium to the degassed water, then add 8kg of algae species t...

Embodiment 3

[0038] Embodiment 3: a kind of method for expanding cultivation of pond mouth expanding cultivation algal species of the present invention, adopts following process step:

[0039] Step 1, medium preparation: take 60 parts of sodium nitrate, 8 parts of potassium dihydrogen phosphate, 5 parts of magnesium sulfate, 15 parts of sodium carbonate, 3 parts of citric acid, 4 parts of sodium silicate, 3 parts of boric acid, 2 parts of manganese chloride part; the above-mentioned raw materials were stirred and mixed by a twin-shaft paddle mixer at normal temperature for 50 minutes, and the rotating speed was 60 revolutions per minute to obtain the culture medium for expanding and cultivating algae species at the pond mouth, and set aside;

[0040] Step 2, remove residual chlorine: take a 90L clean empty barrel, fill it with tap water, and explode for 32 hours to remove residual chlorine;

[0041] Step 3, preparation of mixed bacterial solution: Add 0.3kg of the above-mentioned culture m...

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PUM

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Abstract

The invention relates to a culture medium for algae seed culture expansion at a pond mouth and a culture expansion method and belongs to the technical field of aquaculture. The culture medium comprises sodium nitrate, mono potassium phosphate, magnesium sulfate, sodium carbonate, citric acid, sodium silicate, boric acid and manganese chloride. The raw materials are stirred and mixed according to aproportion and packaged so as to obtain the culture medium for algae seed culture expansion at the pond mouth. The culture medium and algae seeds are added to a barrel filled with water for culture expansion, and culture expansion can succeed under the proper conditions. The culture medium meets the growth demand of the algae seeds, and accordingly quick growth of the algae seeds can be promoted.The culture expansion method is simple, tap water after aeration is used for reducing introduction of exogenous hybrid algae, through continuous aeration in the culture expansion process, the algae can continuously receive illumination and carries on the photosynthesis, algae sedimentation is avoided, and the culture expansion success rate is increased.

Description

technical field [0001] The invention relates to a culture medium and a method for expanding cultivation of algal species at pond mouths, and belongs to the technical field of aquaculture. Background technique [0002] Algae plays an important role in aquaculture, and it is indispensable for maintaining the normal function of the aquatic ecosystem and stabilizing the pond water environment. During the sustainable development of the algae population, on the one hand, it provides sufficient dissolved oxygen for the water body through photosynthesis, on the other hand, it reduces and eliminates organic pollution and other harmful substances in the aquaculture water body, maintains a virtuous cycle of the aquaculture ecosystem, and achieves the purpose of improving water quality . Therefore, rational use of algae in aquaculture can often achieve twice the result with half the effort. Farmers pay more and more attention to the cultivation of algae. Generally, they will add benef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王明星黄忠平
Owner 无锡中顺生物技术有限公司