Bacillus aryabhattai and application thereof in hay modulation

A technology of Bacillus arborii and oat hay, applied in the field of animal husbandry, can solve the problems of slow release of cellulose and hemicellulose, high lignin content, and low utilization rate

Inactive Publication Date: 2019-08-09
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of high lignin content, slow release of cellulose and hemicellulose, low utilization rate and easy mold in oat hay, and provide a kind of Bacillus arborii and its preparation in hay application

Method used

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  • Bacillus aryabhattai and application thereof in hay modulation
  • Bacillus aryabhattai and application thereof in hay modulation
  • Bacillus aryabhattai and application thereof in hay modulation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Inoculate Bacillus arzii from the strain preservation tube into LB broth medium, culture in a shaker flask at 120 rpm min-1, activate at 35°C for 24 h, take 50 μL of the activated seed solution and inoculate it into the alkaline lignin single carbon Cultivate in the culture medium at 35 °C for 24 h, centrifuge at 3000 rpm to discard the supernatant, take a little sludge into the LB broth medium, and culture in a shaking flask at 120 rpm min-1 for 8 h, so that the final number of viable bacteria reaches 5× 109 CFU·ml-1, centrifuge at 3000 rpm to discard the supernatant, add an equal amount of 0.9% normal saline to obtain a suspension of Bacillus arzii.

[0027] How to use the fungicide:

[0028] Harvest the oats at the milky stage, leave stubble of 10 cm to mow, spread it into grass strips, and then evenly spray the suspension of Bacillus arborii. · ml-1, while applying 0.10 mol·L-1, or 0.12 mol·L-1, or 0.13 mol·L-1 magnesium oxide to promote lignin degradation, Bacillu...

Embodiment 2

[0030] Inoculate Bacillus arborii from the strain preservation tube into LB broth medium, culture in shake flask at 120 rpm·min-1, activate at 35°C for 24 h, take 50 μL of activated seed solution and inoculate it into sodium lignosulfonate culture Centrifuge at 3000 rpm for 24 h, discard the supernatant, take a little sludge into LB broth, and culture in a shaker flask at 120 rpm min-1 for 8 h, so that the final number of viable bacteria reaches 5×109 CFU·ml-1, centrifuge at 3000 rpm to discard the supernatant, add an equal amount of 0.9% normal saline to obtain a suspension of Bacillus arzii.

[0031] How to use the fungicide:

[0032] Harvest at the stage of oat milk maturity, leave 10 cm of stubble to mow, spread it into grass strips, and then evenly spray the suspension of Bacillus arborii. -1, apply sodium propionate at a concentration of 1%, or 0.8%, or 1.2% at the same time, Bacillus arborii exerts its degradative effect during the natural drying period, degrades the l...

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Abstract

The invention discloses bacillus aryabhattai for decomposing lignocellulose and application thereof in hay preparation and belongs to the technical field of animal husbandry. The bacillus aryabhattaiis preserved at the General Microbiology Center of China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC No.14522, and the preservation date is Aug.15, 2017. The invention further discloses application of the bacillus aryabhattai in hay preparation. Hay is preferably oat hay. The microbial agent can remarkably reduce the lignin content in the hay preparation process, and cellulose and hemicellulose are released, so that the digestibility of animals is indirectly improved, and molds are inhibited.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and relates to a bacillus arborii for decomposing lignocellulose and its application in hay preparation. Background technique [0002] Lignocellulose is the most abundant and cheapest renewable resource on earth, and it is abundant in various forage grasses. Hemicellulose can be completely fermented in the rumen, and part of the cellulose and a large amount of lignin cannot be rapidly degraded by rumen microorganisms, losing feeding value. Cellulose is a natural polymer compound, and its hydrolysis can produce monosaccharides or disaccharides such as glucose that are easily absorbed by animals. [0003] As a green degradation technology, biodegradation is very popular in the field of animal husbandry. In recent years, the screening, development and utilization of probiotics have become increasingly frequent. Whether it is the bacterial agent added to the feed or the bacterial agent dire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/18C12R1/07
CPCA23K10/12A23K10/18C12N1/205C12R2001/07
Inventor 许庆方熊乙玉柱史悦欧翔马菱艺梁琪李金俐
Owner SHANXI AGRI UNIV
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