Method of preparing fermented soymilk via Paenibacillus bovis, fermented soymilk prepared via Paenibacillus bovis and application of fermented soymilk
A technology of bacillus and soybean milk, which is applied in the preparation of fermented soybean milk, fermented soybean milk and its application fields, can solve the problems of oral flora imbalance, lack of fermented products, strong side effects, etc., and achieve the effect of inhibiting activity
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[0021] In a specific embodiment, a method for preparing fermented soybean milk is provided, comprising the following steps: inoculating Paenibacillus bovis CGMCC No.8333 into soybean milk for fermentation to obtain fermented soybean milk.
[0022] In the preparation method of the above technical solution, Paenibacillus bovis (Paenibacillus bovis) CGMCC No.8333 is used as the fermentation agent for the first time, and soybean milk is used as the medium for fermentation to prepare fermented soybean milk with Streptococcus mutans biofilm inhibitory activity, bovine The metabolites synthesized by Paenibacillus bovis CGMCC No.8333 from soybean milk have inhibitory activity against Streptococcus mutans, and the new application of fermented soybean milk from Paenibacillus bovis CGMCC No.8333 to prevent dental caries is disclosed. Compared with other strains that can ferment soybean milk or the growth medium of the strain, the fermented soybean milk prepared by CGMCC No.8333 has a more...
Embodiment 1
[0037] 1. Materials and methods
[0038] (a) Preparation of Paenibacillus bovis seeds (fermentation strain): Dissolve the freeze-dried powder of Paenibacillus bovis CGMCC No.8333 with a small amount of sterile distilled water, and take a ring with an inoculation loop and streak it on the TYC solid medium ( Purchased from OXOID Co., UK), 30°C aerobic culture for 48h, took out, picked a single colony with an inoculation loop and put it into 10mL TYC liquid medium (purchased from OXOID Co., UK), and used a vortex shaker to evenly disperse the colony in In the liquid medium, shake culture at 30°C and 180rpm for 24h, take it out, inoculate it in TYC liquid medium (purchased from OXOID Co., UK) with a 2% (v / v) inoculum size, and cultivate it at 30°C and 180rpm for 24h, then culture Centrifuge at 15000rpm for 10 minutes, discard the supernatant, wash the thalline twice with sterile distilled water, suspend with the sterile distilled water of the original culture volume, and obtain the ...
Embodiment 2
[0050] 1. Materials and methods
[0051] (a) Preparation of Paenibacillus bovis seed (fermentation strain) and Streptococcus mutans bacterial suspension: Same as Example 1.
[0052] (b) Preparation of soymilk: After weighing the dried red bean, add five times the mass of water and soak at 37°C for 8 hours, discard the water, add a certain proportion of wet beans and water to the soymilk machine to obtain the desired Soybean milk with solid content was sterilized at 121°C for 20 minutes to obtain aseptic red bean soymilk with specified solid content.
[0053] (c) Determination of the inhibitory rate of fermented soybean milk to Streptococcus mutans biofilm formation: Same as Example 1.
[0054] 2. Preparation of fermented soybean milk
[0055] Paenibacillus bovis (Paenibacillus bovis) CGMCC No.8333 was aseptically inoculated in red bean soybean milk with a solid content of 9% (w / w) at an inoculum size of 1% (v / v), and cultured statically at 15°C for 48 hours to obtain fermen...
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