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Method of preparing fermented soymilk via Paenibacillus bovis, fermented soymilk prepared via Paenibacillus bovis and application of fermented soymilk

A technology of bacillus and soybean milk, which is applied in the preparation of fermented soybean milk, fermented soybean milk and its application fields, can solve the problems of oral flora imbalance, lack of fermented products, strong side effects, etc., and achieve the effect of inhibiting activity

Active Publication Date: 2019-08-16
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the research reports on dental caries treatment focus on controlling or reducing the number of Streptococcus mutans in the oral cavity. However, a healthy oral environment is generally in a state where harmful bacteria and beneficial bacteria reach a balance. Excessive reduction of harmful bacteria will Bring the imbalance of oral flora, and then cause other adverse reactions of the human body
In addition, some clinically used bactericidal drugs against Streptococcus mutans, such as silver ammonium nitrate and other compounds, have strong side effects, and long-term use will stain teeth, so they are not suitable for anterior teeth treatment
The risk of dental caries can also be reduced by reducing the production of Streptococcus mutans biofilm without disrupting the inherent balance of oral flora or causing side effects, but there are relatively few reports in this regard, especially the research related to microbial fermentation products is extremely limited , fermented products that inhibit the biofilm formation of Streptococcus mutans are quite scarce

Method used

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  • Method of preparing fermented soymilk via Paenibacillus bovis, fermented soymilk prepared via Paenibacillus bovis and application of fermented soymilk
  • Method of preparing fermented soymilk via Paenibacillus bovis, fermented soymilk prepared via Paenibacillus bovis and application of fermented soymilk
  • Method of preparing fermented soymilk via Paenibacillus bovis, fermented soymilk prepared via Paenibacillus bovis and application of fermented soymilk

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preparation example Construction

[0021] In a specific embodiment, a method for preparing fermented soybean milk is provided, comprising the following steps: inoculating Paenibacillus bovis CGMCC No.8333 into soybean milk for fermentation to obtain fermented soybean milk.

[0022] In the preparation method of the above technical solution, Paenibacillus bovis (Paenibacillus bovis) CGMCC No.8333 is used as the fermentation agent for the first time, and soybean milk is used as the medium for fermentation to prepare fermented soybean milk with Streptococcus mutans biofilm inhibitory activity, bovine The metabolites synthesized by Paenibacillus bovis CGMCC No.8333 from soybean milk have inhibitory activity against Streptococcus mutans, and the new application of fermented soybean milk from Paenibacillus bovis CGMCC No.8333 to prevent dental caries is disclosed. Compared with other strains that can ferment soybean milk or the growth medium of the strain, the fermented soybean milk prepared by CGMCC No.8333 has a more...

Embodiment 1

[0037] 1. Materials and methods

[0038] (a) Preparation of Paenibacillus bovis seeds (fermentation strain): Dissolve the freeze-dried powder of Paenibacillus bovis CGMCC No.8333 with a small amount of sterile distilled water, and take a ring with an inoculation loop and streak it on the TYC solid medium ( Purchased from OXOID Co., UK), 30°C aerobic culture for 48h, took out, picked a single colony with an inoculation loop and put it into 10mL TYC liquid medium (purchased from OXOID Co., UK), and used a vortex shaker to evenly disperse the colony in In the liquid medium, shake culture at 30°C and 180rpm for 24h, take it out, inoculate it in TYC liquid medium (purchased from OXOID Co., UK) with a 2% (v / v) inoculum size, and cultivate it at 30°C and 180rpm for 24h, then culture Centrifuge at 15000rpm for 10 minutes, discard the supernatant, wash the thalline twice with sterile distilled water, suspend with the sterile distilled water of the original culture volume, and obtain the ...

Embodiment 2

[0050] 1. Materials and methods

[0051] (a) Preparation of Paenibacillus bovis seed (fermentation strain) and Streptococcus mutans bacterial suspension: Same as Example 1.

[0052] (b) Preparation of soymilk: After weighing the dried red bean, add five times the mass of water and soak at 37°C for 8 hours, discard the water, add a certain proportion of wet beans and water to the soymilk machine to obtain the desired Soybean milk with solid content was sterilized at 121°C for 20 minutes to obtain aseptic red bean soymilk with specified solid content.

[0053] (c) Determination of the inhibitory rate of fermented soybean milk to Streptococcus mutans biofilm formation: Same as Example 1.

[0054] 2. Preparation of fermented soybean milk

[0055] Paenibacillus bovis (Paenibacillus bovis) CGMCC No.8333 was aseptically inoculated in red bean soybean milk with a solid content of 9% (w / w) at an inoculum size of 1% (v / v), and cultured statically at 15°C for 48 hours to obtain fermen...

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Abstract

The invention discloses a method of preparing fermented soymilk via Paenibacillus bovis. The method comprises the steps of inoculating Paenibacillus bovis CGMCC No. 8333 as a fermenting bacterial agent to soymilk, and carrying out fermenting to obtain fermented soymilk. Fermented soymilk prepared via the method and application of the fermented soymilk in resistance to dental caries are also disclosed. The fermented soymilk prepared herein helps significantly reduce the formation quantity of Streptococcus mutans biofilm. A novel use of the fermented milk prepared via the Paenibacillus bovis CGMCC No. 8333 in resistance to dental caries is disclosed herein; the fermented milk has a good application prospect. Compared with other strains with a soymilk fermenting function or culture media which the strains may grow and breed, the Paenibacillus bovis CGMCC No. 8333 enables the fermented soymilk prepared therewith to gain the activity of significantly inhibiting the formation of Streptococcus mutans biofilm.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of fermented soybean milk. In addition, the invention also relates to a fermented soybean milk and its application. Background technique [0002] Dental caries, commonly known as caries, tooth decay, is a bacterial disease, can be secondary to pulpitis and periodontitis, and even cause alveolar bone and jaw inflammation. If it is not treated in time, the lesion will continue to develop, forming cavities, and eventually the crown will be completely destroyed and disappeared. The final result of its development is tooth loss. Dental caries is characterized by high incidence and wide distribution. The disease is the main common disease of the oral cavity and one of the most common human diseases. The World Health Organization has listed it as one of the three major diseases of human beings, along with tumors and cardiovascular diseases. [0003] There...

Claims

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Application Information

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IPC IPC(8): A23C11/10A23L11/50A23L11/60A23L11/65
CPCA23C11/106A23V2400/169
Inventor 韩瑨乔祯逸吴正钧沈怡帆
Owner BRIGHT DAIRY & FOOD
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