Ganoderma lucidum polysaccharide-based conjugate drug-loaded nano particle with pH/redox dual response and preparation method thereof

A Ganoderma lucidum polysaccharide, drug-loaded nanotechnology, applied in the field of biomedicine and nanomedicine, to achieve good biocompatibility, reduce damage, and improve cycle time

Active Publication Date: 2019-08-30
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the study of stimuli-responsive n...

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  • Ganoderma lucidum polysaccharide-based conjugate drug-loaded nano particle with pH/redox dual response and preparation method thereof
  • Ganoderma lucidum polysaccharide-based conjugate drug-loaded nano particle with pH/redox dual response and preparation method thereof
  • Ganoderma lucidum polysaccharide-based conjugate drug-loaded nano particle with pH/redox dual response and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Weigh 350mg of rutin into a 10mL dry three-necked flask, add 5mL of N,N-dimethylformamide DMF, and continuously inject N into the three-necked flask 2 , add 100mg APBA, and react at room temperature for 24 hours; after the reaction is completed, drop the reaction solution into chloroform to precipitate a precipitate, wash the precipitate three times with chloroform, filter to obtain a solid, and dry the solid in vacuum for 12 hours to obtain a rutin-phenylboronic acid combination thing;

[0031] 2. Weigh 100mg dithiodipropionic acid and dissolve it in 5mL DMSO, add 140mg carbodiimide (EDC) and 90mg N-hydroxysuccinimide (NHS) respectively, activate the carboxyl group at room temperature for 3 hours; add 140mg bis Hydrogen artemisinin and 45mg 4-dimethylaminopyridine (DMAP), at room temperature N 2 React under protection for 24 hours. After the reaction, drop the reaction solution into deionized water, precipitate out, and wash repeatedly with deionized water, redisso...

Embodiment 2

[0035] 1. Weigh 300mg of rutin into a 10mL dry three-necked flask, add 5mL N,N-dimethylformamide DMF, and continuously inject N into the three-necked flask 2 , add 150mg APBA, and react at room temperature for 24 hours; after the reaction is completed, drop the reaction liquid into chloroform to precipitate a precipitate, wash the precipitate three times with chloroform, filter to obtain a solid, and dry the solid in vacuum for 12 hours to obtain rutin-phenylboronic acid combined thing;

[0036] 2. Weigh 150mg dithiodipropionic acid and dissolve it in 5mL DMSO, add 210mg carbodiimide (EDC) and 150mg N-hydroxysuccinimide (NHS) respectively, activate the carboxyl group at room temperature for 3 hours; add 300mg bis Hydrogen artemisinin and 80mg 4-dimethylaminopyridine (DMAP), at room temperature N 2 React under protection for 24 hours. After the reaction, drop the reaction solution into deionized water, precipitate out, and wash repeatedly with deionized water, redissolve the s...

Embodiment 3

[0040] 1. Weigh 450mg of rutin into a 10mL dry three-necked flask, add 3mL of N,N-dimethylformamide DMF, and continuously inject N into the three-necked flask 2 , add 150mg APBA, and react at room temperature for 24 hours; after the reaction is completed, drop the reaction liquid into chloroform to precipitate a precipitate, wash the precipitate three times with chloroform, filter to obtain a solid, and dry the solid in vacuum for 12 hours to obtain rutin-phenylboronic acid combined thing;

[0041] 2. Weigh 120mg dithiodipropionic acid and dissolve it in 5mL DMSO, add 170mg carbodiimide (EDC) and 150mg N-hydroxysuccinimide (NHS) respectively, activate the carboxyl group at room temperature for 3 hours; add 120mg bis Hydrogen artemisinin and 90mg 4-dimethylaminopyridine (DMAP), at room temperature N 2 React under protection for 24 hours. After the reaction, drop the reaction solution into deionized water, precipitate out, and wash repeatedly with deionized water, redissolve th...

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Abstract

The invention discloses a ganoderma lucidum polysaccharide-based conjugate drug-loaded nano particle with pH/redox dual response. The ganoderma lucidum polysaccharide-based conjugate drug-loaded nanoparticle is characterized in that the ganoderma lucidum polysaccharide-based conjugate is prepared by chemically linking rutin, phenylboronic acid, ganoderma lucidum polysaccharide, dithiodipropionicacid and dihydroartemisinin to form rutin-phenylboronic acid-ganoderma lucidum polysaccharide-dithiodipropionic acid-dihydroartemisinin. The ganoderma lucidum polysaccharide is a hydrophilic segment,the rutin and dihydroartemisinin are hydrophobic segments, and the rutin-phenylboronic acid-ganoderma lucidum polysaccharide-dithiodipropionic acid-dihydroartemisinin conjugate self-assembles and entraps 10-hydroxycamptothecin in an aqueous solution to form the drug-loaded nanoparticle.

Description

technical field [0001] The invention relates to a ganoderma lucidum polysaccharide-based conjugate drug-loaded nanoparticle with pH / oxidation-reduction dual response and a preparation method thereof, belonging to the technical field of biomedicine and nanomedicine. Background technique [0002] As an important way to deliver cancer chemotherapy drugs, nano drug delivery system has attracted extensive attention of researchers, but the non-specific release of drugs will bring serious toxic side effects to normal tissues. Stimulus-responsive drug release is a common drug delivery strategy for nanosystems, which can be released in diseased tissues or cells, and can be released in specific lesions and specific physiological environments, thereby improving drug distribution in the body. , enhance the therapeutic effect, and reduce the effect of toxic and side effects, and has broad application prospects. [0003] Ganoderma lucidum polysaccharide GLP is a natural polymer polysacch...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K9/51A61K31/366A61K31/4745A61K31/7048A61K31/715A61P35/00C08B37/02
CPCA61K31/4745A61K31/715A61K31/366A61K31/7048A61K9/5161A61K47/61A61P35/00C08B37/0024C08B37/0009A61K2300/00
Inventor 雷建都郑丹赵静养王璐莹刘静何静
Owner BEIJING FORESTRY UNIVERSITY
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