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Holothurian glycosaminoglycan separating and refining device and method

The technology of a sea cucumber polysaccharide and a centrifuge is applied in the field of deep processing of sea cucumber, and can solve the problems of low polysaccharide extraction, waste of water resources, complicated procedures, etc., and achieve the effects of high purity, energy saving, and simple procedure flow.

Pending Publication Date: 2019-09-06
RIZHAO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the method for separating and refining sea cucumber polysaccharides provided in the above-mentioned technical solution still has many disadvantages in actual use, such as complex procedures, and the preparation of sea cucumber polysaccharides also needs to be dissolved and decolorized again, which wastes water resources. In addition, the sea cucumber slurry Low polysaccharide extraction

Method used

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  • Holothurian glycosaminoglycan separating and refining device and method
  • Holothurian glycosaminoglycan separating and refining device and method
  • Holothurian glycosaminoglycan separating and refining device and method

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Effect test

Embodiment 1

[0035] according to Figure 1-5 The device for separating and refining sea cucumber polysaccharides includes a pulverizer 1, the output end of the pulverizer 1 is provided with a pulping mechanism 2, and one side of the pulpmaking mechanism 2 is provided with a purification box 3. The output end of the purification box 3 is provided with a centrifuge 4, the output end of the centrifuge 4 is provided with a water pump 5, one side of the water pump 5 is provided with an ultrafiltration assembly 6, and the output end of the ultrafiltration assembly 6 is provided with an analysis crystal module 7;

[0036] The pulp making mechanism 2 includes a box body 21, a first electric push rod 22 is provided on the inner top of the box body 21, a motor 23 is provided at the output end of the first electric push rod 22, and a motor 23 is provided at the output shaft of the motor 23. Stirring frame 24, described box body 21 inside is provided with heater 25, described box body 21 inside botto...

Embodiment 2

[0051] The invention provides a method for separating and refining sea cucumber polysaccharides, the specific steps are as follows:

[0052] Step 1: Put the dried sea cucumber into the pulverizer 1 and pulverize it into nano-particles, set the particle diameter of the sea cucumber powder to 250 mesh, take 15 parts of sea cucumber powder, 120 parts of water, and a calcium hydroxide solution with a concentration of 10% in proportion 11 parts and 0.03 parts of composite enzymes are put into casing 21, and described sea cucumber composite enzymes are made by trypsin, pepsin, flavor protease and collagenase combination, start the first electric push rod 22, motor 23 and heater 25, heat Stir the sea cucumber compound solution, set the pH value of the compound solution to 7.5, set the heating temperature to 60°C, set the enzymolysis treatment time to 4 hours, and let the sea cucumber powder stand after gelatinization and enzymolysis;

[0053] Step 2: After standing still in step 1, t...

Embodiment 3

[0060] The invention provides a method for separating and refining sea cucumber polysaccharides, the specific steps are as follows:

[0061] Step 1: Put the dried sea cucumber into the pulverizer 1 and pulverize it into nanoparticles, set the particle diameter of the sea cucumber powder to 300 mesh, take 20 parts of sea cucumber powder, 150 parts of water, and a calcium hydroxide solution with a concentration of 10% in proportion 20 parts and 0.04 parts of composite enzymes are put into casing 21, and described sea cucumber composite enzymes are made by the combination of trypsin, pepsin, flavor protease and collagenase, start the first electric push rod 22, motor 23 and heater 25, heat Stir the sea cucumber compound solution, set the pH value of the compound solution to 8, set the heating temperature to 70°C, set the enzymolysis treatment time to 5 hours, and let the sea cucumber powder stand after gelatinization and enzymolysis;

[0062] Step 2: After standing still in step ...

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Abstract

The invention discloses a holothurian glycosaminoglycan separating and refining device and method, and particularly relates to the technical field of trepang deep processing. The device comprises a smasher. A pulp manufacturing mechanism is arranged at the output end of the smasher. A purifying box is arranged at one side of the pulp manufacturing mechanism, a centrifugal machine is arranged at the output end of the purifying box, a water pump is arranged at the output end of the centrifugal machine, an ultra-filtration assembly is arranged at one side of the water pump, a crystallization assembly is arranged at the output end of the ultra-filtration assembly, the pulp manufacturing mechanism comprises a box, and a first electric push rod is arranged at the top of the inner side of the box. Trepang powder is sufficiently gelled and enzymatically hydrolyzed, polysaccharide is dissolved into water, a calcium hydroxide solution and nitrogen gas are added, polysaccharide molecules are stable, after activated carbon is used for color removal and peculiar smell removal, active carbon granules and large-granule impurities are centrifuged, the purity of the holothurian glycosaminoglycan water solution is improved due to multi-stage ultrafiltration, water is distilled to concentrate holothurian glycosaminoglycan, high-purity holothurian glycosaminoglycan powder is separated out throughsecondary alcoholizaition, the extracting rate and purity are high, working procedures are simplified, and consumed time is short.

Description

technical field [0001] The invention relates to the technical field of sea cucumber deep processing, more specifically, the invention relates to a device and method for separating and refining sea cucumber polysaccharides. Background technique [0002] Sea cucumber (Sea Cucumber) is a traditional valuable tonic in my country, which is rich in various bioactive substances and has high edible and medicinal value. In my country, people's demand for sea cucumbers and sea cucumber products is increasing with the improvement of living standards. Sea cucumber polysaccharides are one of the most important biologically active substances in sea cucumbers, which have various activities such as anticoagulant, antithrombotic, hypolipidemic, and tumor suppressor. [0003] The Chinese patent of patent application publication number CN 106117382 A discloses a method for separating and refining sea cucumber polysaccharides. The sea cucumber raw material is taken for pulping to obtain sea cu...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈涛
Owner RIZHAO POLYTECHNIC