Coding gene for enhancing iron deficiency tolerance and accumulation of plant and application of coding gene

A technology that encodes genes and plants, applied in the field of bioengineering, can solve problems such as time-consuming and labor-intensive efficacy, inconspicuousness, and lack of iron nutrition

Inactive Publication Date: 2019-09-06
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the iron deficiency of plants, it is impractical to increase the iron content in the soil. This method is time-consuming and labor-intensive and the effect is not obvious. The improvement effect of relying on iron supplements can only maintain short-term effects, so molecular breeding is used Introduce ge

Method used

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  • Coding gene for enhancing iron deficiency tolerance and accumulation of plant and application of coding gene
  • Coding gene for enhancing iron deficiency tolerance and accumulation of plant and application of coding gene
  • Coding gene for enhancing iron deficiency tolerance and accumulation of plant and application of coding gene

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, ZIP44 Identification of Transgenic Plant Transcript Level and Its Response to Iron Deficiency

[0027] Compared with the use of MS medium without iron to screen the Arabidopsis seed mutant library, a mutant strain sensitive to iron deficiency was obtained, and the mutant was found to be ZIP44 loss-of-function mutants, named bzip44 . Because the seed is a T-DNA insertion mutation, it is amplified by PCR with specific primers and sequenced. The sequencing results are compared in the NCBI database Blast, and the T-DNA insertion site information is obtained through comprehensive analysis. See figure 1 . Using qRT-PCR, the bzip44 The mutants were identified at the transcript level, and it was found that in the two mutants ZIP44 The expression levels of the genes were significantly lower than those of the wild type, see figure 2 . see image 3 In order to further determine ZIP44 Whether the gene responds to iron deficiency stress in plants, we e...

Embodiment 2

[0029] Embodiment 2, cultivating iron-deficiency-tolerant Arabidopsis

[0030] 1, ZIP44 Gene Overexpression Transgenic Lines bZIP44-OE1, OE2 the acquisition

[0031] To further verify the function of this gene in the regulation of iron deficiency stress in plants, we constructed ZIP44 Gene overexpression vector ( 35S: bZIP44 ). Firstly, the target fragment is amplified. The wild-type Arabidopsis was normally cultured on MS medium for two weeks, the total RNA of the plant was extracted, reverse-transcribed to synthesize cDNA, and the synthesized cDNA was used as a template for PCR to amplify a sufficient amount of the target product. Using the PCR product as a template, the second amplification was carried out for the purpose of introducing enzyme cutting sites. The PCR product and vector pCAMBIA1301 were digested and recovered. Then, the recovered and purified target DNA fragment and the vector were ligated overnight with T4 DNA ligase. The above ligation solution ...

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Abstract

The invention relates to a coding gene for enhancing iron deficiency tolerance and accumulation of a plant and application of the coding gene. The sequence of the coding gene for enhancing iron deficiency tolerance and accumulation of the plant is as shown in the sequence table SEQ ID No: 1. The coding gene for enhancing iron deficiency tolerance and accumulation of the plant is transferred into the plant, the coding gene for enhancing iron deficiency tolerance and accumulation of the plant is over-expressed in the plant, so that the tolerance of the plant to iron deficiency is displayed in vivo. The coding gene for enhancing iron deficiency tolerance and accumulation of the plant can provide gene resources and technical support for breeding crops under iron deficiency stress tolerance.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a gene encoding a protein related to iron-deficiency stress resistance in plants, and at the same time, relates to the use of the gene to enhance the tolerance of plants to an iron-deficiency environment, thereby possibly positively regulating the response of plants to iron-deficiency stress . Background technique [0002] Iron is a metal element with an atomic number of 26, located in the fourth period of the periodic table, and is one of the most commonly used metals in life. Iron is an essential trace element for the survival of humans, animals and plants. In plants, iron is the trace element most needed by plants and plays an important role in the life activities of plants, such as participating in the synthesis of plant chlorophyll, photosynthesis and respiration. Although the content of iron in the soil is high, due to the very active chemical properties of iron an...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H6/20
CPCC07K14/415C12N15/8271
Inventor 曹树青吴席朱香豫王媛媛陶曼芝江力樊婷婷盛义保
Owner HEFEI UNIV OF TECH
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