A preparation method of a ratiometric electrochemical biosensor for detecting aflatoxin b1
An aflatoxin and sensor technology, applied in the field of biosensors, to achieve the effects of reducing toxin interference, high sensitivity and improving accuracy
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Embodiment 1
[0032] according to figure 1 The preparation process described above, the preparation method of the ratiometric electrochemical biosensor comprises the following steps:
[0033] (1) Preparation of THI-rGO composites:
[0034] First, weigh 1mg THI and dissolve it in ultrapure water to obtain a concentration of 20μg·mL in 50mL -1 THI solution, and serially diluted to 5 μg·mL -1 , stored away from light at room temperature; weigh 3mg rGO and disperse it in 30mL ultrapure water to obtain a concentration of 0.1mg·mL -1 The rGO dispersion solution was treated with a cell pulverizer for 30 min and stored at room temperature until use.
[0035] Then, take 3mL of 5μg·mL -1 THI with 15mL 0.1mg·mL -1 Mix rGO in a 100mL beaker and stir for 12h. After the stirring is over, put the solution in a 10mL centrifuge tube. Under the conditions of rotating speed of 8000rpm and centrifugation time of 15min, centrifuge and wash three times to obtain a precipitate
[0036] Finally, the obtained...
Embodiment 2
[0044] Optimized experimental conditions for the prepared ratiometric electrochemical biosensor for detection of aflatoxin B1:
[0045] The biosensor prepared in Example 1 was placed at 4° C. for 6, 8, 10, 12, and 14 hours to detect Fc electrochemical signals.
[0046] figure 2 (A) AFB1 aptamer incubation time and I Fc Relationship diagram, the incubation time of the aptamer increased from 6h to 10h, I Fc Gradually increased and reached a plateau at 10h. Therefore, 12h was chosen as the optimal incubation time for aptamers.
Embodiment 3
[0048] Optimized experimental conditions for the prepared ratiometric electrochemical biosensor for detection of aflatoxin B1:
[0049] On the basis of the ratio biosensor with the best conditions obtained in Example 2, modify the target AFB1 on the surface of the sensor, such as figure 2 (B) is the target incubation time and I Fc Relationship diagram, the incubation time of the target on the electrode surface is from 0.3h to 1.0h, I Fc The signal gradually decreased, and after 1h I Fc There was no significant change in the signal, so the incubation time of the selected target AFB1 was 1.3h.
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