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A degradable miRNAs-loaded nanocomposite coating and its preparation method and application

A nano-composite coating and coating technology, applied in coatings, pharmaceutical formulations, surgery, etc., can solve the problems of high off-target effect, low drug delivery and effectiveness, and achieve a wide range of applications and achieve biological functionalization. The effect of modifying and improving cell membrane penetration

Active Publication Date: 2021-11-02
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects and deficiencies of high off-target effects, low drug delivery and effectiveness in the application of non-coding small nucleic acid sequences in surface coatings in the prior art, and to provide a degradable nanometer loaded with miRNAs. Construction method of composite coating

Method used

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  • A degradable miRNAs-loaded nanocomposite coating and its preparation method and application
  • A degradable miRNAs-loaded nanocomposite coating and its preparation method and application
  • A degradable miRNAs-loaded nanocomposite coating and its preparation method and application

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Embodiment 1

[0062] see figure 1 , the present embodiment provides a method for constructing a degradable nanocomposite coating loaded with miRNAs, the steps of which are:

[0063] A. Construction of miRNAs-loaded nanoparticles: mix equal volumes of polylysine solution (PLL, molecular weight 150-300KDa) with a concentration of 1mg / mL and miRNAs mimics solution with a concentration of 1nmol / mL, and let it stand for 1 hour at room temperature Finally, under the condition of magnetic stirring, the mixed solution was added dropwise to an equal volume of heparin sodium solution with a concentration of 5 mg / mL to obtain nanoparticles loaded with miRNAs.

[0064] B. Modification of miRNAs-loaded nanoparticles: First, centrifuge the nanoparticle suspension obtained in step A at a speed of 12000 rpm, discard the supernatant, and resuspend in an equal volume of MES buffer with a concentration of 0.05mol / L Second, add NHS and EDC powders to the suspension in turn, so that the molar ratio of EDC, NHS...

Embodiment 2

[0068] This embodiment provides a method for constructing a degradable nanocomposite coating loaded with miRNAs, the steps of which are:

[0069] A. Construction of miRNAs-loaded nanoparticles: mix equal volumes of polylysine solution (PLL, molecular weight 150-300KDa) with a concentration of 10 mg / mL and miRNAs mimics solution with a concentration of 10 nmol / mL, and statically treat at room temperature for 6 hours Finally, under the condition of magnetic stirring, the mixed solution was added dropwise to an equal volume of heparin sodium solution with a concentration of 50 mg / ml, so as to obtain nanoparticles loaded with miRNAs.

[0070] B. Modification of miRNAs-loaded nanoparticles: first, centrifuge the nanoparticle suspension obtained in step A at a speed of 12000 rpm, discard the supernatant, and resuspend in an equal volume of MES buffer with a concentration of 0.05M; Next, add NHS and EDC powders to the above suspension in turn, so that the molar ratio of EDC, NHS and ...

Embodiment 3

[0074] This embodiment provides a method for constructing a degradable nanocomposite coating loaded with miRNAs, the steps of which are:

[0075] A. Construction of miRNAs-loaded nanoparticles: Mix the polylysine solution (PLL, molecular weight 150-300KDa) with a concentration of 5 mg / mL and the miRNAs mimics solution with a concentration of 5 nmol / mL in equal volumes, and statically treat at room temperature for 3 hours Finally, under the condition of magnetic stirring, the mixed solution was added dropwise into an equal volume of heparin sodium solution with a concentration of 25 mg / mL to obtain nanoparticles loaded with miRNAs.

[0076] B. Modification of miRNAs-loaded nanoparticles: first, centrifuge the nanoparticle suspension obtained in step A at a speed of 12000 rpm, discard the supernatant, and resuspend in an equal volume of MES buffer with a concentration of 0.05M; Next, add NHS and EDC powders to the above suspension in turn, so that the molar ratio of EDC, NHS and...

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Abstract

The invention relates to a degradable nanocomposite coating loaded with miRNAs, a preparation method and application thereof. The construction method includes the following steps: S1: construction of miRNAs-loaded nanoparticles; S2: modification of miRNAs-loaded nanoparticles; S3: deposition and thiolation of polydopamine coating; S4: miRNAs-loaded nanoparticles / hyaluronic acid hydrogel Preparation of adhesive composite coatings. Through the construction of the coating, the invention can realize the controlled release and intracellular delivery of miRNAs-loaded nanoparticles, selectively inhibit blood coagulation reaction and excessive proliferation of smooth muscle, promote the growth of endothelial cells, and effectively improve the biocompatibility of materials.

Description

technical field [0001] The invention belongs to the field of preparation technology of degradable polymer materials and nanometer materials and surface modification technology of biomedical materials, and specifically relates to a degradable nanocomposite coating loaded with miRNAs and its preparation method and application. Background technique [0002] Biomedical metal materials (such as 316L stainless steel, cobalt alloys, titanium and titanium alloys, etc.) are widely used in cardiovascular implant materials and devices (such as vascular stents, thrombus filters, etc.). However, complications such as surface coagulation reaction and endothelial dysfunction caused by material or device implantation often lead to implant failure. For example, the implantation of coronary drug-eluting stents will cause delayed healing of endothelial injury, which will lead to late thrombosis and intimal hyperplasia, endangering the life of patients. Therefore, it is of great research value...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/10A61L31/14A61L31/16
CPCA61L31/10A61L31/14A61L31/145A61L31/148A61L31/16A61L2300/252A61L2300/258A61L2300/412A61L2300/42A61L2400/18A61L2420/04C08L77/04C08L79/02C08L5/08
Inventor 刘涛许能贵唐纯志刘诗卉崔韶阳霍青伟陶蓉蓉
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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