Application of Populus trichocarpa PtrMYB119 gene in improvement of drought resistance of tobacco

A technology of Populus trichocarpa and drought tolerance, applied in the field of plant genetic engineering, can solve the problems of crop growth and development hindrance, plant yield reduction, economic loss, etc., and achieve the effect of improving drought resistance and increasing anthocyanin content

Active Publication Date: 2019-09-20
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Drought will cause the water content of plant cells to drop, the outbreak of active oxygen free radicals, damage the plant cell membrane system, cause plant growth retardation, and even death, resulting in plant yield reduction
At present, more than one-third of the land in the world belongs to arid and semi-arid regions. Drought has always been one of the main reasons affecting agricultural development. Nowadays, the scope, degree and frequency of drought disasters are increasing, which affects the growth and development of crops. Serious hindrance, reduced crop yields causing huge economic losses

Method used

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  • Application of Populus trichocarpa PtrMYB119 gene in improvement of drought resistance of tobacco
  • Application of Populus trichocarpa PtrMYB119 gene in improvement of drought resistance of tobacco
  • Application of Populus trichocarpa PtrMYB119 gene in improvement of drought resistance of tobacco

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Embodiment 1

[0043] (1) Extract the total RNA of Populus trichocarpa, obtain cDNA after reverse transcription, and amplify the PtrMYB119 gene coding sequence from the poplar cDNA by PCR technology. The specific process is: use the following nucleotide sequences as primers: PtrMYB119-F: 5'-CTAAGGAAGTGCGTTGAGAA-3', PtrMYB119-R: 5'-GCCAAGCAACTTGTGTAGTC-3', for PCR amplification. Amplification conditions are 94°C, 3min; 30 cycles, each cycle 94°C, 30s, 60°C, 30s, 72°C, 1min; 72°C final extension 10min; reaction system is 20μL: cDNA template 1μL, upstream and downstream primers each 0.4 μL (10 μM), 10×Buffer 2 μL, dNTPs 1.6 μL, rTaq enzyme 0.1 μL, ddH 2 O 14.5 μL. The PCR products were subjected to agarose gel electrophoresis, and the gel extraction kit was used to recover the gel by tapping. The recovered products were stored at -20°C after the concentration was measured, or directly subjected to enzyme digestion, ligation and other reactions.

[0044] (2) Inserting the obtained PtrMYB119 ge...

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Abstract

The invention provides application of a Populus tomentosa PtrMYB119 gene in improvement of drought resistance of tobacco, and belongs to the technical field of plant genetic engineering. According to the research, the Populus tomentosa PtrMYB119 gene is transferred into tobacco leaves, the anthocyanin content in the obtained transgenic tobacco is remarkably improved, and the drought resistance of the tobacco can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of Populus trichocarpa PtrMYB119 gene in improving drought tolerance of tobacco. Background technique [0002] Drought will lead to a decrease in the water content of plant cells, the outbreak of active oxygen free radicals, damage to the plant cell membrane system, resulting in stunted growth and even death of plants, resulting in reduced plant production. At present, more than one-third of the land in the world belongs to arid and semi-arid areas. Drought has always been one of the main reasons affecting agricultural development. Nowadays, the scope, degree and frequency of drought disasters are increasing, which affects the growth and development of crops. Serious hindrance, crop yield reduction causes huge economic loss. Improving the drought resistance of plants has become one of the key issues in modern plant research. The study of drought...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/825C12N15/8273
Inventor 庄维兵王鸿雪刘天宇王忠王涛张凤娇束晓春
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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