Composition, kit and method for detecting mycoplasma ovipneumoniae

A technology of Mycoplasma pneumoniae and a composition, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high technical requirements, complicated operation and high price, and achieve excellent technical effects and simple operations.
CN110257539APending Publication Date: 2019-09-20NINGXIA UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
NINGXIA UNIVERSITY
Publication Date
2019-09-20

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Abstract

The invention discloses a composition, a kit and a method for rapidly detecting mycoplasma ovipneumoniae. The accurate detection of mycoplasma ovipneumoniae is completed by designing specific primers and probes of ketolase gene of mycoplasma ovipneumoniae. The best reaction time and the best reaction temperature in the detection process, the specificity, sensitivity, repeatability and stability of the detection are all explored, the best reaction condition is found, and a rapid detection method of mycoplasma ovipneumoniae with good specificity, high sensitivity and stable repetition is established; and the method is simple to operate, convenient and time-saving, does not need large-scale experimental instruments and equipment, is very suitable for personnel without any experimental basis to operate, and realizes on-site detection.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology, in particular to a composition, a kit and a method for rapid detection of ovine mycoplasma pneumoniae. Background technique

[0002] Mycoplasma pneumonia of sheep and goats (MPSG) is a highly contagious infectious disease transmitted through air, droplets, drinking water, etc. The main clinical symptoms are panting, coughing, high fever, progressive weight loss, and chronic hyperplastic stroma pneumonia etc. The pathogenic bacteria that have been found to cause mycoplasma pneumonia include Mycoplasma ovipneumoniae (Mycoplasma ovipneumoniae, MO), Mycoplasma capricolum subsp.capricolum (Mcc), Mycoplasma mycoides subsp.capri (Mmc) Among them, MO is one of the common pathogenic bacteria. The disease is widely distributed and popular in my country, and the infection rate is high, which seriously affects the sheep breeding industry. Therefore, it is very important to establish an accurate det...

Claims

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