Composition, kit and method for detecting mycoplasma ovipneumoniae

Abstract

The invention discloses a composition, a kit and a method for rapidly detecting mycoplasma ovipneumoniae. The accurate detection of mycoplasma ovipneumoniae is completed by designing specific primers and probes of ketolase gene of mycoplasma ovipneumoniae. The best reaction time and the best reaction temperature in the detection process, the specificity, sensitivity, repeatability and stability of the detection are all explored, the best reaction condition is found, and a rapid detection method of mycoplasma ovipneumoniae with good specificity, high sensitivity and stable repetition is established; and the method is simple to operate, convenient and time-saving, does not need large-scale experimental instruments and equipment, is very suitable for personnel without any experimental basis to operate, and realizes on-site detection.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a composition, a kit and a method for rapid detection of ovine mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumonia of sheep and goats (MPSG) is a highly contagious infectious disease transmitted through air, droplets, drinking water, etc. The main clinical symptoms are panting, coughing, high fever, progressive weight loss, and chronic hyperplastic stroma pneumonia etc. The pathogenic bacteria that have been found to cause mycoplasma pneumonia include Mycoplasma ovipneumoniae (Mycoplasma ovipneumoniae, MO), Mycoplasma capricolum subsp.capricolum (Mcc), Mycoplasma mycoides subsp.capri (Mmc) Among them, MO is one of the common pathogenic bacteria. The disease is widely distributed and popular in my country, and the infection rate is high, which seriously affects the sheep breeding industry. Therefore, it is very important to establish an accurate det...

AI Technical Summary

Problems solved by technology

Although the PCR method is fast and can replace the culture method in a certain sense, its high sensitivity also makes the PCR method relatively demanding on the experimental environment and instruments, and there are problems such as high technical requirements, complicated operation, and difficulty in popularization. Grass-roots laboratories are difficult to develop, and the price is relatively high

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