Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Composition, kit and method for detecting mycoplasma ovipneumoniae

A technology of Mycoplasma pneumoniae and a composition, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high technical requirements, complicated operation and high price, and achieve excellent technical effects and simple operations.

Pending Publication Date: 2019-09-20
NINGXIA UNIVERSITY
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the PCR method is fast and can replace the culture method in a certain sense, its high sensitivity also makes the PCR method relatively demanding on the experimental environment and instruments, and there are problems such as high technical requirements, complicated operation, and difficulty in popularization. Grass-roots laboratories are difficult to develop, and the price is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition, kit and method for detecting mycoplasma ovipneumoniae
  • Composition, kit and method for detecting mycoplasma ovipneumoniae
  • Composition, kit and method for detecting mycoplasma ovipneumoniae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment is a system based on the amplification reaction of specific gene transketolase. details as follows:

[0037] (1) detection system of the present invention comprises:

[0038]

[0039] Among them, Primer free Rehydration buffer is a known commercially available product.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composition, a kit and a method for rapidly detecting mycoplasma ovipneumoniae. The accurate detection of mycoplasma ovipneumoniae is completed by designing specific primers and probes of ketolase gene of mycoplasma ovipneumoniae. The best reaction time and the best reaction temperature in the detection process, the specificity, sensitivity, repeatability and stability of the detection are all explored, the best reaction condition is found, and a rapid detection method of mycoplasma ovipneumoniae with good specificity, high sensitivity and stable repetition is established; and the method is simple to operate, convenient and time-saving, does not need large-scale experimental instruments and equipment, is very suitable for personnel without any experimental basis to operate, and realizes on-site detection.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a composition, a kit and a method for rapid detection of ovine mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumonia of sheep and goats (MPSG) is a highly contagious infectious disease transmitted through air, droplets, drinking water, etc. The main clinical symptoms are panting, coughing, high fever, progressive weight loss, and chronic hyperplastic stroma pneumonia etc. The pathogenic bacteria that have been found to cause mycoplasma pneumonia include Mycoplasma ovipneumoniae (Mycoplasma ovipneumoniae, MO), Mycoplasma capricolum subsp.capricolum (Mcc), Mycoplasma mycoides subsp.capri (Mmc) Among them, MO is one of the common pathogenic bacteria. The disease is widely distributed and popular in my country, and the infection rate is high, which seriously affects the sheep breeding industry. Therefore, it is very important to establish an accurate det...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/689C12Q1/6816C12Q1/04C12N15/11C12R1/35
CPCC12Q1/689C12Q1/6816C12Q2565/625Y02A40/70
Inventor 李敏郝秀静金华马春骥王玉炯
Owner NINGXIA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com