PCR primer, primer group, probe, kit and detection method for detecting Proteus and Proteus mirabilis

A technology of Proteus mirabilis and detection kits, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of low detection sensitivity, hidden safety hazards, cumbersome operation, etc., and achieve high sensitivity, The effect of reducing workload and simplifying the process
CN110257540APending Publication Date: 2019-09-20ICDC CHINA CDC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ICDC CHINA CDC
Publication Date
2019-09-20

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Abstract

The invention applies to the technical field of bacterial detection, provides a PCR primer, primer group, probe, kit and detection method for detecting Proteus and Proteus mirabilis, and discloses four PCR primer nucleotide sequences, in two pairs, for detecting Proteus and Proteus mirabilis. The kit comprising the primer group features high specificity and high sensitivity, and has a lower detection limit of 102 CFU / mL; detection results are accurate and reliable; electrophoretic detection on a target gene amplified product is not required; operating is simple; detection cycle is short; workload of operating personnel can be decreased; precise detection information can be acquired in time; the process of detecting Proteus species is simplified greatly; the detection cycle is shortened; the detection method may act as a primary screening means of sensitivity; positive rate of Proteus species cultivation and detection is increased; forcible technical support can be provided for China to formulate hygiene indexes for Proteus species in food in future.
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Description

technical field

[0001] The invention belongs to the technical field of bacteria detection, and in particular relates to a PCR primer, a primer set, a probe, a kit thereof and a detection method for detecting Proteus and Proteus mirabilis. Background technique

[0002] Proteus is a non-encapsulated, non-spore-forming, gram-negative bacillus with flagella and pili. It is widely distributed in nature, animal feces, clinical specimens, and the intestinal tract of humans and animals. It is an important cause of human and animal infections. Conditional pathogens. In recent years, the prevalence of Proteus infection has continued to expand. According to the statistical results of food poisoning caused by Proteus reported in relevant literature, it is shown that food poisoning caused by Proteus in my country is on the rise year by year. In addition to causing food poisoning, Proteus can also cause a variety of infections, such as respiratory tract infection, urinary tract infectio...

Claims

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