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Preparation method for compound microorganism preparation for achieving shortcut nitrification

A composite microbial inoculum and short-range nitrification technology, applied in the field of the preparation of composite microbial inoculants, can solve the problems of nitrogen loss, increase in anaerobic fermentation, slow ammonia oxidation rate, etc., and achieve the effect of shortening time and improving degradation rate.

Inactive Publication Date: 2019-09-24
YANGZHOU ZHENWEI BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

[0005] Microbial fermentation and embedding to prepare bacterial agents is a common low-input, high-efficiency bacterial agent treatment method. The effect of biochemical killing and deodorization is not ideal, and long-term composting leads to increased anaerobic fermentation, a large number of reverse digestion bacteria, and a large number of Nitrogen loss
Heterotrophic nitrifying bacteria are the most important components of heterotrophic nitrifying bacteria agents. At present, there are many heterotrophic nitrifying bacteria screened by domestic and foreign scholars, but there is still the problem that the ammonia oxidation rate per unit biomass is slower than that of autotrophic nitrifying bacteria.
In addition, most of the heterotrophic nitrifying bacteria currently screened out often have a relatively high concentration of ammonia nitrogen residues when the concentration of high-concentration ammonia nitrogen wastewater is large.

Method used

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  • Preparation method for compound microorganism preparation for achieving shortcut nitrification

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Embodiment Construction

[0022] The invention discloses a compound microbial bacterial agent prepared by fermenting and immobilizing three strains of bacterial strains screened from landfill leachate sewage, which is used in a sewage treatment biochemical system.

[0023] Prepared by:

[0024] (1) Take Chenjiachong landfill leachate sewage, according to the screening formula (ammonium sulfate 0.5g, sodium nitrite 1.0g, sodium chloride 0.3g, ferrous sulfate 0.03g, dipotassium hydrogen phosphate 0.03g, agar 20g, water 1L) , Prepare the medium required for screening, sterilize at 121°C for 20 minutes, spread the plate, and select the fastest-growing single colony as the strain of the compound microbial agent (that is, pick the microbial strain that grows rapidly within 24 hours of a single colony, Continue to use broth medium to induce culture at low temperature, select the number of microbial strains that grow obvious single colonies within 24 hours, and save them for future use);

[0025] (2) Accordin...

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Abstract

The invention relates to the biochemical treatment section of sewage treatment, in particular to a preparation method for a compound microorganism preparation for achieving shortcut nitrification. The preparation method for the compound microorganism preparation for achieving shortcut nitrification can quickly increase ammonia nitrogen degradation rate and shorten the time for biological removal of nitrogen. The compound microorganism preparation contains three kinds of bacteria with nitrification functions, the compound microorganism preparation is prepared through microbial fermentation and encapsulation immobilization, and the living bacteria count of the compound microorganism preparation is equal to or higher than 1.0*10<9> cfu / g, wherein according to the treatment conditions, the temperature is 20-30 DEG C, pH is 6.0-9.0, and the first inoculation quantity of a system is 0.1-0.3%. When the compound microorganism preparation is used in the biochemical system for sewage treatment, the highest rate of removing ammonia nitrogen in sewage reaches 90%, the ammonia nitrogen degradation rate of the system can be quickly increased, and the time for biological removal of nitrogen is shortened.

Description

technical field [0001] The invention relates to a sewage treatment biochemical treatment section, in particular to a preparation method of a compound microbial bacterial agent for realizing short-range nitrification. Background technique [0002] Ammonia nitrogen is a common pollutant in polluted water bodies, and high concentrations of ammonia nitrogen are often contained in landfill leachate and agricultural wastewater. If the ammonia nitrogen cannot be treated in a timely and effective manner, it will cause great harm to the environment. Therefore, the treatment of ammonia nitrogen in wastewater is a key issue in the treatment of landfill leachate and other sewage. There are generally physical, chemical and biological methods for denitrification of sewage. Biological treatment process is widely used in sewage treatment because of its low treatment cost and no secondary pollution. [0003] The principle of traditional biological denitrification is that ammonia nitrogen is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/02C02F3/34C02F101/16
CPCC02F3/34C02F2101/16C12N1/20C12N11/02
Inventor 张磊
Owner YANGZHOU ZHENWEI BIOTECH CO LTD
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