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Enzyme/manganese phosphate trihydrate/graphene oxide nano composite material and preparation method thereof

A technology of nanocomposite materials and manganese phosphate trihydrate, applied in the field of chemical materials, to achieve the effect of mild and simple experimental process and simple equipment

Active Publication Date: 2019-09-24
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, as far as the field of enzyme-nanomaterials is concerned, it is still in the exploratory stage, and the research on combining enzyme-manganese phosphate trihydrate-graphene oxide to form composite nanomaterials has not been reported yet.

Method used

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  • Enzyme/manganese phosphate trihydrate/graphene oxide nano composite material and preparation method thereof
  • Enzyme/manganese phosphate trihydrate/graphene oxide nano composite material and preparation method thereof
  • Enzyme/manganese phosphate trihydrate/graphene oxide nano composite material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An enzyme / manganese salt / graphene oxide nanocomposite material, comprising the following components in mass percentage: 22.36% of laccase, 73.17% of manganese phosphate trihydrate crystals, and 4.47% of GO.

[0032] (1) Preparation method of nanocomposite materials

[0033] Dissolve 10 mg laccase in PBS (0.2 mol / L) with a pH of 5.7, add 0.667 mL of 120 mM manganese chloride solution and let stand for 18 h, then dissolve 2 mg GO in the above solution, at 25 °C , and reacted in a shaker at 140rpm for 24 h. Then the precipitate was separated, washed and freeze-dried in a vacuum freeze dryer at -20 °C for 6 h. The obtained enzyme / manganese salt / graphene oxide nanocomposite is scanned by electron microscope, and the results are as follows: figure 1 . Depend on figure 1 It can be seen that the composite material presents a unique wheel-shaped flower-like nanostructure.

[0034] (2) Determination method and results of laccase activity in nanocomposite materials

[0035] ...

Embodiment 2

[0039] An enzyme / manganese phosphate trihydrate / graphene oxide nanocomposite material, comprising the following components in mass percentage: 19.18% of carbonic anhydrase, 78.43% of manganese phosphate trihydrate crystals, and 2.40% of GO.

[0040] (1) Preparation method of nanocomposite materials

[0041] Dissolve 8 mg of carbonic anhydrase in PBS (0.2 mol / L) with a pH of 8.5, add 0.667 mL of 120 mM manganese chloride solution and let stand for 16 h, then dissolve 1 mg of GO in the above solution, at 25 °C , and reacted in a shaker at 140rpm for 20 h. Afterwards, the precipitate was separated, washed and freeze-dried in a vacuum freeze-dryer at -20°C for 5 h. Electron microscopy scanning of the obtained enzyme / manganese salt / graphene oxide nanocomposite showed a unique wheel-shaped flower-like nanostructure.

[0042] (2) Determination method and results of carbonic anhydrase activity in nanocomposite materials

[0043]Mix 0.3 mL of the enzyme solution to be tested, 2.4 mL...

Embodiment 3

[0046] An enzyme / manganese phosphate trihydrate / graphene oxide nanocomposite material, comprising the following components in mass percentage: 20.82% of carbonic anhydrase, 75.71% of manganese phosphate trihydrate crystals, and 3.47% of GO.

[0047] (1) Preparation method of nanocomposite materials

[0048] Dissolve 9 mg of carbonic anhydrase in PBS (0.2 mol / L) with a pH of 8.5, add 0.667 mL of 120 mM manganese chloride solution and let it stand for 20 h, then dissolve 1.5 mg of GO in the above solution, at 25 °C , reacted for 24 h in a shaker at 140 rpm, and then the precipitate was separated, washed and freeze-dried in a vacuum freeze-dryer at -20 °C for 7 h. Electron microscopy scanning of the obtained enzyme / manganese salt / graphene oxide nanocomposite showed a unique wheel-shaped flower-like nanostructure.

[0049] (2) Determination method and results of carbonic anhydrase activity in nanocomposite materials

[0050] Mix 0.3 mL of the enzyme solution to be tested, 2.4 mL...

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Abstract

The invention provides an enzyme / manganese phosphate trihydrate / graphene oxide nano composite material and a preparation method thereof, wherein the nano composite material is prepared from the following components in percentage by mass: 15-25% of biological enzyme, 70-80% of manganese phosphate trihydrate crystal and 1-5% of graphene oxide. A dynamic and static binding reaction method is adopted to obtain enzyme / manganese phosphate trihydrate / graphene oxide nano composite material, a microstructure of theenzyme / manganese phosphate trihydrate / graphene oxide nano composite material exhibits a unique wheel-shaped flower-like nanostructure; in terms of performance, the enzyme activity of the immobilized enzyme is improved and storage stability is improved, and technical support is provided for biological enzyme immobilization, industrial catalysis and other fields.

Description

technical field [0001] The invention relates to the technical field of chemical materials, in particular to an enzyme / manganese phosphate trihydrate / graphene oxide nanocomposite material and a preparation method thereof. Background technique [0002] Enzyme is a kind of biocatalyst, which has the advantages of strong selectivity, mild reaction conditions and simple process. Traditional enzyme immobilization methods can improve the performance of certain enzymes. With the rapid development of modern society, various ingenious preparation techniques apply different types of materials to enzyme immobilization, and the performance of enzyme immobilization is getting higher and higher. At present, more and more inorganic materials are used in the field of enzyme immobilization. Among them, graphene oxide (GO) in the graphite family can form a unique three-dimensional space structure with other substances due to its unique nanostructure and excellent performance. If the two are c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
CPCC12N11/14C12N9/0061C12N9/88C12Y110/03002C12Y402/01001
Inventor 段林林盛宏林张亚涛米立伟陈卫华
Owner ZHONGYUAN ENGINEERING COLLEGE
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