Method for detecting single base mutation, and application thereof
A single-base mutation and purpose technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of poor DNA purification effect, poor genetic detection effect, complicated extraction process, etc., to avoid abuse Drugs and adverse drug reactions, predicting disease risk, and effective effects
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Embodiment 1
[0029] A method for detecting a single base mutation, comprising the steps of:
[0030] S1. DNA extraction: extract a small amount of animal or plant tissue, put it into a sterilized test tube, add a lysis solution to the test tube, and place the test tube in a high-speed centrifuge for centrifugation. The time is controlled at 5 minutes, and the lysis solution is specifically 0.1 mol / L sodium chloride solution, 10mol / L Tris-HCl solution and 1% SDS solution, the pH of the lysis solution is 8.0;
[0031] S2, DNA separation; specifically include adding phenol-chloroform solution, chloroform-isoamyl alcohol solution, ice absolute ethanol and 5mol / L sodium chloride solution to the test tube after completing operation S1, and place the test tube again Perform centrifugation on a high-speed centrifuge, and stop centrifugation when white flocculent precipitates appear in the solution in the test tube.
[0032] S3. Purification of DNA: After S2 is completed, add 60% ethanol solution ...
Embodiment 2
[0048] A method for detecting a single base mutation, comprising the steps of:
[0049] S1. DNA extraction: extract a small amount of animal or plant tissue, put it into a sterilized test tube, add a lysis solution to the test tube, and place the test tube in a high-speed centrifuge for centrifugation. The time is controlled at 8 minutes, and the lysis solution is specifically 0.1 mol / L sodium chloride solution, 10mol / L Tris-HCl solution and 1% SDS solution, the pH of the lysis solution is 8.0;
[0050] S2, DNA separation; specifically include adding phenol-chloroform solution, chloroform-isoamyl alcohol solution, ice absolute ethanol and 5mol / L sodium chloride solution to the test tube after completing operation S1, and place the test tube again Perform centrifugation on a high-speed centrifuge, and stop centrifugation when white flocculent precipitates appear in the solution in the test tube.
[0051] S3. Purification of DNA: After S2 is completed, add 65% ethanol solution ...
Embodiment 3
[0067] A method for detecting a single base mutation, comprising the steps of:
[0068] S1. DNA extraction: extract a small amount of animal or plant tissue, put it into a sterilized test tube, add a lysis solution to the test tube, and place the test tube in a high-speed centrifuge for centrifugation. The time is controlled at 10 minutes, and the lysis solution is specifically 0.1 mol / L sodium chloride solution, 10mol / L Tris-HCl solution and 1% SDS solution, the pH of the lysis solution is 8.0;
[0069] S2, DNA separation; specifically include adding phenol-chloroform solution, chloroform-isoamyl alcohol solution, ice absolute ethanol and 5mol / L sodium chloride solution to the test tube after completing operation S1, and place the test tube again Perform centrifugation on a high-speed centrifuge, and stop centrifugation when white flocculent precipitates appear in the solution in the test tube.
[0070] S3, DNA purification: After S2 is completed, add 70% ethanol solution an...
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