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A Strain of Stenotrophomonas Efficiently Degrading Polybutylene Terephthalate/Adipate and Its Application

A polybutylene terephthalate and adipate technology, applied in bacteria, hydrolase, microorganism-based methods, etc., can solve the problems of small types and numbers of microorganisms, slow degradation, and low degradation rate, and achieve excellent Degradation effect, strong adaptability, widely distributed effect

Active Publication Date: 2021-02-02
内蒙古颐祥杰成科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current studies have found that the degree of degradation of PBAT is closely related to the physical and chemical properties of the environment, such as pH, temperature, and humidity. This is reflected in the fact that the degradation rate of PBAT in the northwest region is far lower than that in the southwest region. Moreover, the types and quantities of microorganisms in the environment in the northwest region Less, which makes the application of PBAT severely limited
Therefore, there is an urgent need for an environment-tolerant PBAT-degrading bacteria to solve the problem of low degradation rate of PBAT in different regions.
[0003] At present, there are few studies on PBAT-degrading bacteria. The PBAT-degrading bacteria reported at home and abroad mainly include Sphingopyxis ginsengisoli, Bacillus pumilus, Pseudomonas genus Pseudomonas pseudoalcaligenes) and the fungus Trichoderma asperellum (Trichoderma asperellum), but there is generally a problem of low degradation rate, and the degradation of PBAT by strains is generally slow in an alkaline environment

Method used

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  • A Strain of Stenotrophomonas Efficiently Degrading Polybutylene Terephthalate/Adipate and Its Application
  • A Strain of Stenotrophomonas Efficiently Degrading Polybutylene Terephthalate/Adipate and Its Application
  • A Strain of Stenotrophomonas Efficiently Degrading Polybutylene Terephthalate/Adipate and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The isolation and identification of embodiment 1 bacterial strain

[0035] 1. Plate separation and screening of PBAT-degrading bacteria

[0036] Collect the soil of a farmland with PBAT mulch in Yanxia Town, Liquan County, Shaanxi Province, weigh 10g of soil in a 150mL triangular flask with 90mL of sterile water, and configure 10 -1 ~10 -6 The soil bacteria suspension with a series of concentration gradients was inoculated in the inorganic salt medium with PBAT as the only carbon source. After culturing in a constant temperature incubator at 37°C for 3 days, the growth of microorganisms on the medium was observed, and a single colony was picked. Continuously inoculated several times in PBAT as the only carbon source inorganic salt medium for isolation and purification until a strain of bacteria was isolated, and then the colony morphology was observed, as shown in figure 1 As shown, the colonies were orange-red, with flat edges and smooth surfaces.

[0037] 2. Identi...

Embodiment 2

[0040] Example 2 strain producing lipase ability identification

[0041] The Stenotrophomonas was inoculated on the oil medium, cultured at 37°C for 3 days, and there were obvious red hydrolysis circles under the colonies, which showed that the bacteria could produce lipase.

Embodiment 3

[0042]Example 3 Degradation experiment of strains on PBAT membrane and lipase production experiment.

[0043] 1. Preparation of PBAT membrane

[0044] Select 5 g of PBAT film with a molecular weight of 100,000, dissolve it in 95 mL of analytically pure chloroform, stir it thoroughly until it dissolves, pour it into a mold, wait for the chloroform to volatilize, prepare a PBAT film, and cut it into 1 *1cm 2 A small piece of the strain was used for the experiment of degrading the PBAT membrane.

[0045] 2. Preparation of Medium

[0046] On the basis of PBAT as the sole carbon source inorganic salt medium, 3% butanediol and other external carbon sources were added as the co-metabolism growth substrate of the bacteria.

[0047] 3. Preparation of Seed Solution

[0048] The purified strain was inoculated in 100 mL of LB medium, and cultured for 12 hours at 37°C with a shaker speed of 130 r / min to obtain OD 600 The bacterial solution with a value of 1.0 was used as the seed solu...

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Abstract

The invention provides a strain of Stenotrophomonas that efficiently degrades polybutylene terephthalate / adipate and the application thereof. The present invention isolates and screens a PBAT-degrading bacterium that can secrete lipase, Stenotrophomonas sp.strain YCJ1, from the soil of Yanxia Town, Liquan County, Shaanxi Province, and names it Stenotrophomonas sp. Species Collection and Management Center, the deposit number is CGMCC 18059. The advantage of the present invention is that the bacterium can secrete lipase and efficiently degrade PBAT, and the bacterium has a better degradation effect under alkaline conditions, and the Stenotrophomonas and its lipase can be used as enzyme preparations and biological agents It has a good application value when applied to the environmental remediation of PBAT and PBAT-based biodegradable materials in alkaline environment.

Description

technical field [0001] The invention belongs to the technical field of PBAT degradation, and in particular relates to a strain of Stenotrophomonas sp.strain YCJ1 that efficiently degrades PBAT. Background technique [0002] Polybutylene terephthalate / adipate (PBAT) is an aliphatic biodegradable material, because it has good mechanical properties, thermoplasticity and performance of traditional plastics, and at the same time, PBAT can be destroyed by microorganisms in the environment. It is degraded by lipase and has good biodegradability, which makes PBAT widely used in agriculture, food and packaging industries. However, current studies have found that the degree of degradation of PBAT is closely related to the physical and chemical properties of the environment, such as pH, temperature, and humidity. This is reflected in the fact that the degradation rate of PBAT in the northwest region is far lower than that in the southwest region. Moreover, the types and quantities of m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12N9/20A62D101/28C12R1/01
CPCA62D3/02A62D2101/28C12N9/20C12Y301/01003C12N1/205C12R2001/01
Inventor 张敏贾昊李成涛
Owner 内蒙古颐祥杰成科技有限责任公司
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