Lipoprotein electrophoresis staining solution and preparation method and application thereof

Abstract

The invention provides a lipoprotein electrophoresis staining solution and a preparation method and an application thereof. The lipoprotein electrophoresis staining solution comprises a lipid dye, anorganic solvent, a cosolvent and water. In the preparation process of the lipoprotein electrophoresis staining solution provided by the invention, the lipid dye can be completely dissolved, on the onehand, the loss of the lipid dye is avoided, and on the other hand, the concentration of the completely dissolved lipid dye can be calculated, so that accurate quantification can be realized during use. In addition, the lipoprotein electrophoresis staining solution provided by the invention is long in validity period and good in dyeing effect, the staining of the lipoprotein is more obvious, the resolution ratio is higher, and the qualitative and quantitative analysis of lipoprotein subtypes is more accurate.

Description

technical field [0001] The invention belongs to the field of biomedicine detection, and in particular relates to a lipoprotein electrophoresis staining solution and a preparation method and application thereof. Background technique [0002] In lipoprotein electrophoresis analysis, lipid dyes are often used for staining analysis. Due to the extremely poor water solubility of lipid dyes, the preparation process of lipoprotein electrophoresis staining solutions commonly used is cumbersome and time-consuming, and often takes several hours or even It takes dozens of hours of preparation time, and it is difficult to completely dissolve the dye during the preparation of the staining solution. It is often necessary to centrifuge and / or filter to remove excess dye residue, resulting in the uncertainty of the final concentration of the dye, and it is difficult to accurately quantify the amount of dye when used; and The traditional lipoprotein electrophoresis staining solution has unev...

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Problems solved by technology

[0002] In lipoprotein electrophoresis analysis, lipid dyes are often used for staining analysis. Due to the extremely poor water solubility of lipid dyes, the preparation process of lipoprotein electrophoresis staining solutions commonly used is cumbersome and time-consuming, and often takes several hours or even It takes dozens of hours of preparation time, and it is difficult to completely dissolve the dye during the preparation of the staining solution. It is often necessary to centrifuge and / or filter to remove excess dye residue, resulting in the uncertainty of the final concentration of the dye, and it is difficult to accurately quantify the amount of dye when used; and The traditional lipoprotein electrophoresis staining solution has uneven solute dispersion, poor stability, easy precipitation, short shelf life, and it is difficult to maintain the staining effect for a long time. It can only be stored for a few days to dozens of days, and the storage temperature is limited. Reduce the solubility of lipid dyes, so it is difficult to store at low temperature, and they are mostly stored at room temperature; in addition, the traditional lipoprotein electrophoresis staining solution takes a long time to stain lipoproteins, which often takes tens of minutes to several hours. , because the lipid dye is difficult to miscible with water, the lipid dye will be separated from the solution during pre-staining (such as the pre-staining of plasma), which will not only reduce the staining effect of lipoproteins, but also often need to be centrifuged before loading Remove the sediment, increase the workload

In short, the traditional lipoprotein electrophoresis staining solution and its preparation method not only cause a lot of waste of manpower and material resources, but also easily lead to serious errors in lipoprotein staining analysis

[0003] CN102181173A discloses a Sudan Black B staining solution and its preparation method and application. The Sudan Black B staining solution contains ethylene glycol or glycerol and isopropanol, and the total amount of ethylene glycol or glycerol and isopropanol is In terms of volume, the volume percentage of isopropanol contained therein is greater than or equal to 60%. The Sudan Black B staining solution provided by this patent has good stability and dispersibility in the solution, but it still cannot be stored stably for a long time, and the preparation time Longer, centrifugation is still required during the preparation process, so it is still impossible to quantitatively prepare the dye

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