Colloidal gold kit for detecting diabetic nephropathy biomarker and method

Abstract

The invention provides a colloidal gold kit for detecting a diabetic nephropathy biomarker and a method. The kit comprises a sample pad, a gold standard pad, a nitrocellulose membrane, an absorbent pad and a support pad. The nitrocellulose membrane is provided with a first detection reagent strip, a second detection reagent strip, a third detection reagent strip, a fourth detection reagent strip,and a capture reagent strip successively. The first detection reagent strip coats an anti-IV-type collagen monoclonal antibody; the second detection reagent strip coats an anti-podocalyxin monoclonalantibody; the third detection reagent strip coats an anti-neutropil gelatinase-associated lipocalin monoclonal antibody; the fourth detection reagent strip coats an anti-liver fatty acid binding protein monoclonal antibody; and the capture reagent strip coats a second antibody binding the monoclonal antibody coated on the gold standard pad. According to the invention, with the colloidal gold kit,simultaneous and quantitative detection of various biomarkers of early diabetic nephropathy can be realized; the cost for detecting early diabetic nephropathy is saved; and the detection efficiency isimproved.

Description

technical field [0001] The invention belongs to the field of biomarker detection, in particular to a colloidal gold kit and method for detecting diabetic nephropathy biomarkers. Background technique [0002] Diabetic nephropathy is one of the most serious chronic microvascular complications of diabetes, which eventually leads to end-stage renal failure and is the main cause of death in diabetic patients. However, it is difficult to detect early diabetic nephropathy, and creatinine and proteinuria, the gold standard for clinical diagnosis of diabetic nephropathy, can only indirectly reflect renal parenchymal lesions, and early diabetic nephropathy cannot be diagnosed during normal proteinuria. Usually when clinically diagnosed, diabetic nephropathy patients miss the best opportunity for treatment, resulting in a sharp deterioration of the disease, which is irreversible. Therefore, early detection and early intervention of early injury biomarkers of diabetic nephropathy have ...

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a colloidal gold kit for detecting biomarkers of diabetic nephropathy, which aims to solve the problem that current detection equipment cannot quantitatively detect multiple biomarkers of early diabetic nephropathy at the same time

Images