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A kind of flavonoid monoterpene compound and its preparation method and application

A technology of compound and monoterpene, applied in the field of natural medicinal chemistry, can solve the problem that the antibacterial mechanism has not been studied, and achieve the effect of strong DPPH free radical scavenging ability

Active Publication Date: 2021-06-15
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is only recorded in the literature that the author isolated and identified a few simple compounds (such as cemetine, quercetin and carotene, etc.) from the leaves of the plant, and these compounds were only tested for the activity of two bacteria, Escherichia coli and Bacillus subtilis , and the antibacterial mechanism has not been studied

Method used

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  • A kind of flavonoid monoterpene compound and its preparation method and application
  • A kind of flavonoid monoterpene compound and its preparation method and application
  • A kind of flavonoid monoterpene compound and its preparation method and application

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Experimental program
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Embodiment 1

[0030] The preparation of embodiment 1 grammatin A

[0031] 1. Heat and reflux the dried whole grass powder of C. gramatus with 80% ethanol solution to extract 3 times, each time for 2 hours, filter and combine the extracts, recover ethanol under reduced pressure, then add water to disperse and then use etc. Petroleum ether, ethyl acetate and n-butanol were extracted to obtain petroleum ether phase, ethyl acetate phase and n-butanol phase, respectively.

[0032] 2. The petroleum ether phase extract (35.5g) obtained in 1. was subjected to silica gel column chromatography, and then eluted by petroleum ether-acetone gradient, the volume ratio was changed from 100:1 to 0:1, and thin-layer chromatography was inspected. According to the order of elution, they were combined into 5 components, and the second component was subjected to reverse-phase C8 silica gel column chromatography, and then eluted by methanol-water gradient system, and the volume ratio was changed from 30:70 to 100...

Embodiment 2

[0044] The mensuration of embodiment 2 DPPH free radical scavenging ability

[0045] Accurately weigh 4mg of DPPH and dissolve it in methanol to a 100mL volumetric flask, which is the DPPH solution.

[0046] Take compound (grammatin A, 2mg / mL) 0.1, 0.2, 0.3, 0.4, 0.5mL (make up the insufficient volume to 0.5mL with methanol) and 2mL DPPH solution in the same test tube, shake well. Place it in the dark for 30min, and measure the absorbance at 517nm with the corresponding blank solution as a control. The formula for calculating the DPPH free radical inhibition rate is as follows:

[0047] Inhibition rate% = [A 0 -(A b -A * )] / A 0 *100%

[0048] Among them: A 0 is the absorbance after reaction without sample inhibitor;

[0049] Ab is the absorbance after adding the sample inhibitor to the reaction;

[0050] A * is the absorbance of no inhibitor and DPPH.

[0051] After the above experiments, it was found that the compound has a strong ability to scavenge DPPH free radi...

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Abstract

The invention relates to a flavonoid and monoterpenoid compound and its preparation method and application. Its structural formula is as follows: the compound is isolated from Chimonanthus gramatus M.C.Liu. In vitro biological activity experiments show that the compound has strong 1,1-diphenyl-2-picrylhydrazine free radical (DPPH) scavenging ability, and is a candidate compound of natural antioxidant and free radical scavenger.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and relates to a flavonoid and monoterpenoid compound, in particular to a new flavonoid and monoterpene compound isolated from the plant Prunus chinensis and its preparation method and application. Background technique [0002] Free radicals refer to those molecules, ions, atoms or atomic groups that exist free and contain one or more unpaired electrons. They are the products of the normal metabolism of the body and have strong oxidation reaction capabilities in the body. , It is easy to cause damage to proteins, lipids and nucleic acids, thereby causing damage to the body. Free radicals are also indispensable active substances in the body, and they can act as second messengers to participate in cell signal transduction. Under normal circumstances, the oxidation and anti-oxidation of the body are in a dynamic balance, but in the state of illness or aging, pathological phenomena will ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40A61P39/06
CPCA61P39/06C07D311/30C07D311/40
Inventor 孙振亮黎瑞红蹇洪
Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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