Processing method and application of fish meal in glutamine transaminase fermentation culture medium

A technology of transglutaminase and fermentation medium, which is applied in the fields of microbial fermentation and enzyme engineering, can solve the problems of low enzyme production, low utilization rate of fish meal components, low utilization rate of fish meal and the like, and achieves the effect of easy operation.

Inactive Publication Date: 2019-10-22
TAIXING DONGSHENG FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, because the utilization rate of fishmeal components in the existing fermentation medium is not high, and the enzyme production is relatively low, it is necessary to optimize the components of the medium in a certai

Method used

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  • Processing method and application of fish meal in glutamine transaminase fermentation culture medium
  • Processing method and application of fish meal in glutamine transaminase fermentation culture medium
  • Processing method and application of fish meal in glutamine transaminase fermentation culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0085] The preparation method of the fermentation medium of transglutaminase is as follows (comprising the fish meal treatment step in the fermentation medium of hydrolyzed transglutaminase):

[0086] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0087] 2) Adjusting the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the pH to 3.0 with hydrochloric acid to obtain the mixed system 2;

[0088] 3) Hydrolysis: Put the mixed system 2 under the condition of 121° C. for 60 minutes, take it out, and obtain the fishmeal component in the transglutaminase fermentation medium after cooling, namely the mixed system 3.

[0089] 4) pH adjustment: Stir the mixed system 3 with a magnetic stirrer, and adjust the corresponding pH to the effective pH range as shown in Table 1 with hydrochloric acid or sodium hydroxide to obtain the mixed system 4;

[0090] 5) Enzyme addition: adding hydrolase in proportion by weight to obtain m...

Embodiment 2

[0106] The preparation method of the fermentation medium of transglutaminase is as follows (comprising the fish meal treatment step in the fermentation medium of hydrolyzed transglutaminase):

[0107] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0108] 2) Adjusting the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the pH to 3.0 with hydrochloric acid to obtain the mixed system 2;

[0109] 3) Hydrolysis: Put the mixed system 2 under the condition of 121° C. for 60 minutes, take it out, and obtain the fishmeal component in the transglutaminase fermentation medium after cooling, namely the mixed system 3.

[0110] 4) pH adjustment: Stir the mixed system 3 with a magnetic stirrer, and adjust the corresponding pH to the action pH range as shown in Table 2 with hydrochloric acid or sodium hydroxide to obtain the mixed system 4;

[0111] 5) Enzyme addition: adding hydrolase in proportion by weight to obtain mixe...

Embodiment 3

[0128] The preparation method of the fermentation medium of transglutaminase is as follows (comprising the fish meal treatment step in the fermentation medium of hydrolyzed transglutaminase):

[0129] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0130] 2) Adjusting the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the pH to 3.0 with hydrochloric acid to obtain the mixed system 2;

[0131] 3) Hydrolysis: Put the mixed system 2 under the condition of 121 degrees Celsius for 60 minutes, take it out, and obtain the fishmeal component in the transglutaminase fermentation medium after cooling, namely the mixed system 3.

[0132] 4) pH adjustment: Stir the mixed system 3 with a magnetic stirrer, and adjust the corresponding pH to the effective pH range as shown in Table 3 with hydrochloric acid or sodium hydroxide to obtain the mixed system 4;

[0133] 5) Enzyme addition: adding hydrolase in proportion by weight...

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Abstract

The invention provides a processing method and application of fish meal in a glutamine transaminase fermentation culture medium, discloses a fish meal hydrolysis method and application of the method in fermentative production of glutamine transaminase, and mainly solves the problems of insufficient fish meal hydrolysis and low fish meal utilization rate of an existing fish meal hydrolysis process.Improvement is performed on the basis of an existing fish meal hydrolysis process, wherein the fish meal is subjected to pulverization, impurity removal, acid hydrolysis, alkali hydrolysis, enzymatichydrolysis and the like so that nutrients in the fish meal can be better utilized, and the fish meal utilization rate is increased. The hydrolysis process is also applied to the fermentative production of the glutamine transaminase, which can significantly improve the utilization rate of the fish meal by glutamine transaminase bacteria, and the produced glutamine transaminase has the advantages of high enzyme activity, high stability and the like. The method is simple, convenient, efficient, low in cost and easy to use in industrial production.

Description

technical field [0001] The invention relates to the field of microbial fermentation and enzyme engineering, in particular to a transglutaminase fermentation medium and a preparation method and application thereof. Background technique [0002] Transglutaminase, a transferase that catalyzes the acyl transfer reaction, can promote protein intramolecular cross-linking, protein intermolecular cross-linking and cross-linking between proteins and amino acids, which can greatly improve protein The functional properties of protein can improve the nutritional value of protein, and it has a wide range of applications in the food processing industry. Therefore, the production of transglutaminase has huge market demand and application development value. Transglutaminase was first discovered in the liver of guinea pigs. It was first isolated from Streptomyces maoyuan in 1989. It was produced on a large scale in 1997. It was officially listed as a food additive in my country in 2001. Tr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/10C12R1/465
CPCC12N1/20C12N9/1044C12Y203/02013
Inventor 步国建甘李张玉如寇雪蕊和超楠刘艳贺晓洁
Owner TAIXING DONGSHENG FOOD TECH
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