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Simple and mass-produced green fluorescent carbon point and preparation method and application thereof

A mass production, green fluorescence technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-optics, etc., can solve the problems of strict temperature requirements, difficult to prepare in large quantities, and long reaction time, and achieve low toxicity and excellent biological phase. The effect of capacitive, good fluorescence performance

Inactive Publication Date: 2019-10-29
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the usual carbon dot preparation methods are limited by the capacity of the container, it is difficult to prepare in large quantities, and the reaction time is long and the temperature is strict. Therefore, there is an urgent need for a preparation method that is simple and fast, and the yield is not limited by the container. Mass production Preparation method of carbon dots

Method used

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  • Simple and mass-produced green fluorescent carbon point and preparation method and application thereof
  • Simple and mass-produced green fluorescent carbon point and preparation method and application thereof
  • Simple and mass-produced green fluorescent carbon point and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0024] Utilize citric acid and basic fuchsin to prepare the method for green fluorescent carbon point, comprise the following steps:

[0025] (1) Weigh 5 g of citric acid and 0.3 g of basic fuchsin, mix citric acid and basic fuchsin directly on the heating platform, and keep stirring under the condition that the temperature of the heating platform is controlled to be 300 ° C. The reactant is obtained in minutes, and the reactant contains carbon dots;

[0026] (2) After naturally cooling the reactant obtained in step (1), dissolve the reacted solid in ethanol, centrifuge 10min at a speed of 8000rpm in a high-speed centrifuge to obtain a supernatant; then filter the supernatant into Filter through a 0.22 μm microporous membrane, evaporate the filtrate in an oven at 60°C, and obtain carbon dots after the ethanol volatilizes. Finally, the carbon dot powder was dispersed with ultrapure water to obtain a carbon dot dispersion liquid, which was stored at 4°C.

[0027] The carbon do...

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Abstract

The invention discloses a simple and mass-produced green fluorescent carbon point and a preparation method and application thereof. The preparation method comprises the steps that citric acid and basic fuchsin are directly mixed in a certain proportion, and a carbon point is prepared through a direct heating method. The preparation method is easy, convenient and fast, the yield is high and up to 80%or above, the carbon point can be obtained by direct heating and melting, the preparation method is not limited by a container, and mass production can be achieved. The synthesized carbon point hasbright green fluorescence and good optical stability and biocompatibility, and can be used in the fields of cell imaging or other biological research, and can be used as an optical image probe to be applied to the field of biomedicine.

Description

technical field [0001] The invention relates to the field of carbon dot synthesis, in particular to a simple mass-producible green fluorescent carbon dot, its preparation method and application. Background technique [0002] Carbon dots were discovered by accident in 2004 as a by-product of the synthesis of single-walled carbon nanotubes. It is a fluorescent carbon material composed of dispersed spherical nanoparticles, with a size below 10nm. It is usually composed of four basic elements: C, H, O, and N. From the first discovery to the artificial synthesis, carbon dots have been extensively studied by researchers around the world, and their application fields have also been greatly expanded. Carbon dots are easy to prepare, have excellent optical stability and good biocompatibility, low toxicity and easy surface modification. Researchers have done a lot of work in exploring the luminescent mechanism, exploring the physical and chemical properties, improving the quantum y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65G01N21/6428G01N21/6486
Inventor 王斌刘沛陈久存
Owner SOUTHWEST UNIV
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